Summary
A comparison of citric acid fermentations in manganese-deficient and manganese-containing media showed that manganese strongly influences idiophase metabolism. In the presence of manganese, cell growth increases, sugar consumption is diminished and acidogenesis decreases drastically. An investigation of the key enzymes of glycolysis, the pentosephosphate pathway, TCA-cycle, nitrogen metabolism, and gluconeogenesis indicated that manganese deficiency was accompanied by a repression of anabolic and TCA-cycle-enzymes with the exception of citrate synthase. The activity of this enzyme and the enzymes of glycolysis paralleled the sugar consumption rate. In the presence of manganese, no repression of enzyme synthesis was observed. Activities of 2-oxoglutarate dehydrogenase and isocitrate lyase could not be detected in either case. The results support the hypothesis that manganese deficiency mainly affects the operation of biosynthetic reactions inAspergillus niger, thus leading to an overflow of citric acid as an end product of glycolysis.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Anfinsen, C.B. (1955) Methods in Enzymology, Vol. 1, 695–99, (S.P. Colowick and N.O. Kaplan, eds.) New York: Academic Press
Bergmeyer, H.U., Bernt, E. (1974) Meth. Enzym. Analysis 1. Weinheim: Verlag Chemie
Bücher, T., Pfleiderer, G. (1955) Methods in Enzymology, Vol. 1, 435, (S.P. Colowick and N.O. Kaplan, eds.) New York: Academic Press
Caltrider, P. G., Gottlieb, D. (1963) Phytopathology53, 1021
Clark, D.S., Ito, K., Horitsu, H. (1966) Biotechnol. Bioeng.8., 465
Dixon, G.H., Kornberg, H.L. (1959) Biochem. J.72, 3p
Doelle, H.W. (1975) Bacterial Metabolism. New York, London: Academic Press
Ellis, H.A. (1959) Arch. Biochem. Biophys.85, 561
Galbraight, J.C., Smith, J.E. (1969) J. Gen. Microbiol.59, 31
Hanson, R.S., Cox, D.P. (1967) J. Bact.93, 1777
Horecker, B.L., Smyrniotis, P.Z. (1955) Methods in Enzymology Vol. 1, 371, (S.P. Colowick and N.O. Kaplan, eds.) New York: Academic Press
Horitsu, H. (1971) Hakko Kyokaishi29, 460–464
Jagannathan, V., Singh, K., Damodaran, M. (1956) Biochem. J.64, 477
Khouw, B.T., McCurdy, H.D. (1969) J. Bact.99, 197
Kitto, G.B., Kottge, M.E., Bertland, L.H., Murphey, W.H., Kaplan, N.O. (1967) Arch. Biochem. Biophys.121, 224
Kornberg, A. (1955) Methods in Enzymology, Vol. 1, 735 (S.P. Colowick and N.O. Kaplan, eds.) New York: Academic Press
Lakshminayarana, K., Modi, V.V., Shah, V.K. (1969) Arch. Mikrobiol.66, 406
Müller, H.M., Frosch, S. (1975) Arch. Mikrobiol.104, 159
Ng, A.M.L., Smith, J.E., McIntosh, A.F. (1973) Trans. Br. Mycol. Soc.61, 13
Ng, A.M.L., Smith, J.E., McIntosh, A.F. (1974) J. Gen. Microbiol.81, 425
Ng, W.S., Smith, J.E., Anderson, J.G. (1972) J. Gen. Microbiol.71, 495
Ochoa, S. (1955) Methods in Enzymology Vol. 1, 735 (S.P. Colowick and N.O. Kaplan, eds.) New York: Academic Press
Perlman, D., Sih, C.J. (1960) Progress Ind. Microbiol.2, 168
Rosen, O.M. (1966) Arch. Bioch. Biophys.114, 31
Shu, P., Johnson, M.J. (1948) J. Bact.56, 577
Slater, E.C. (1953) Biochem. J.52, 157
Smith, J.E., Nowakowska-Waszuk, A., Anderson, J.G. (1974) Ind. Aspects Biochem.1, 297
Srere, P.A., Kosicki, G.W. (1961) J. Biol. Chem.236, 2557
Tomlinson, N., Campbell, J.J.R., Trussell, P.C. (1950) J. Bact.59, 217
Verhoff, F.H., Spradlin, J.E. (1976) Biotechnol. Bioeng.18, 425
Waygood, E.R., Rohringer, R. (1964) Mod. Meth. Plant. Analysis 7, 546 (H.F. Linskens and M.V. Tracey, eds.) Berlin-Heidelberg-New York: Springer
Weinberg, E.D. (1970) Adv. Microbiol Phys., Vol. 4, 1, (A.H. Rose and J.F. Wilkinson, eds.) New York: Academic Press
Zonneveld, B.J.M. (1975) Arch. Mikrobiol.105, 101
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Kubicek, C.P., Röhr, M. Influence of manganese on enzyme synthesis and citric acid accumulation inAspergillus niger . European J. Appl Microbiol. 4, 167–175 (1977). https://doi.org/10.1007/BF01390476
Received:
Issue Date:
DOI: https://doi.org/10.1007/BF01390476