Biotechnology Letters

, Volume 1, Issue 11, pp 465–470 | Cite as

A deactivation model involving pH for immobilized and soluble enzymes

  • Ajit Sadana
Article

Abstract

A pH-dependent deactivation model is developed for immobilized and soluble enzymes in which the rate of enzyme decay is assumed proportional to the concentration of theactive enzyme. The model developed applies reasonably well to pH-stability data of Johnson and coworkers (1977 a,b,c; 1978 a,b,c).

Keywords

Enzyme Soluble Enzyme Deactivation Model Enzyme Decay 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. Engasser, J. M., and Horvath, C., (1976) Dynamic Role of Buffers in Heterogeneous Enzyme Systems In:Analysis and Control of Immobilized Enzyme Systems, D. Thomas and J. P. Kernevez, eds. pp 187–197, Amsterdam: North HollandGoogle Scholar
  2. Flynn, A., and Johnson, D. B., (1977a),Int. J. Biochem. 8,501–503Google Scholar
  3. Flynn, A., and Johnson, D. B., (1977b),Int. J. Biochem. 8,243–247CrossRefGoogle Scholar
  4. Flynn, A., and Johnson, D. B., (1978a),Biotech. Bioeng. 20. 1445–1454Google Scholar
  5. Higgins, A. C., and Johnson, D. B., (1977c),Int. J. Biochem. 8, 807–809Google Scholar
  6. Johnson, D. B. (1978b),Biotech. Bioeng., 20, 1117–1123Google Scholar
  7. Johnson, D. B., and Coughlan, M. P., (1978c),Biotech. Bioeng. 20, 1085–1095Google Scholar
  8. Laidler, K. J., and Bunting, P.S., (1973),The Chemical Kinetics of Enzyme Action, Oxford: Clarendon PressGoogle Scholar
  9. Levy, M., and Benaglia, A. E., (1950),J. Biol. Chem. 186, 829–847PubMedGoogle Scholar
  10. Lewis, P.S., (1926a),J. Biochem., 20, 965–977Google Scholar
  11. Lewis, P.S., (1926b),J. Biochem., 20, 984–992Google Scholar
  12. Segel, I. H., (1975),Enzyme Kinetics: Behaviour and Analysis of Rapid Equilibrium and Steady-State Enzyme Systems. Ch.11 Effects of pH and temperature, New York : Wiley and Sons.Google Scholar
  13. Steinhardt, J., (1937),Kgl. Danske Videnskab. Selskab Math-fys. Medd.,14, No.11Google Scholar
  14. Whitaker, J. R. (1972),Principles of Enzymology for the Food Sciences, Ch.10.1 Effect of pH on Stability of Enzymes, New York: Marcel DekkerGoogle Scholar

Copyright information

© Kluwer Academic Publishers 1979

Authors and Affiliations

  • Ajit Sadana
    • 1
  1. 1.National Chemical LaboratoryPoonaIndia

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