Protoplasma

, Volume 171, Issue 1–2, pp 55–63

Immunolocalization of H+-ATPases in the plasma membrane of pollen grains and pollen tubes ofLilium longiflorum

  • G. Obermeyer
  • M. Lützelschwab
  • H. -G. Heumann
  • M. H. Weisenseel
Article

Summary

A heterogeneous distribution of H+-ATPase was visualized in germinated pollen ofLilium longiflorum using monoclonal antibodies raised against plasma membrane H+-ATPase. Immunolocalization studies of protoplasts and subprotoplasts derived from pollen tubes and sectioned pollen grains and pollen tubes show that H+-ATPases are abundant in the plasma membrane of pollen grains but are absent or sparsely distributed in the plasma membrane of pollen tubes. This polar distribution of H+-ATPases is probably the basis of the endogenous current pattern measured in growing lily pollen and involved in pollen tube tip growth.

Keywords

Immunolocalization H+-ATPase Tip growth Lilium longiflorum 

Abbreviations

BSA

bovine serum albumine

Hepes

N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid

Mes

2-(N-morpholino)-ethane sulphonic acid

PBS

phosphate buffered saline

Pipes

piperazine-N,N′-bis(2-ethanesulfonic acid)

Tris

2-amino-2-hydroxymethyl-1,3-propandiol

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • G. Obermeyer
    • 1
  • M. Lützelschwab
    • 2
  • H. -G. Heumann
    • 1
  • M. H. Weisenseel
    • 1
  1. 1.Botanisches Institut IUniversität KarlsruheKarlsruhe
  2. 2.Institut für Biologie IIIUniversität FreiburgFreiburg
  3. 3.Institut für PflanzenphysiologieUniversität SalzburgSalzburgAustria

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