Archives of Virology

, Volume 131, Issue 1–2, pp 89–99 | Cite as

Polymerase chain reaction for detection of herpesvirus simiae (B virus) in clinical specimens

  • M. J. Slomka
  • D. W. G. Brown
  • J. P. Clewley
  • A. M. Bennett
  • L. Harrington
  • D. C. Kelly
Original Papers
Received:
Accepted:

Summary

A polymerase chain reaction (PCR) was designed which is specific toMacaca fascicularis (cynomolgus monkey) isolates of B virus. The PCR primers produced the expected 188 basepair product from the Cyno 2 strain and seven other cynomolgus monkey isolates of B virus. Oligomer hybridization with a 31-mer oligonucleotide was used to confirm the origin of this product. The PCR failed to amplify DNA of Epstein-Barr virus, cytomegalovirus, varicella-zoster virus, and other alphaherpesviruses (herpes simplex virus types 1 and 2, four SA 8 isolates and three rhesus isolates of B virus). PCR testing of swabs obtained from four orally-infected cynomolgus monkeys confirmed the presence of B virus DNA in samples previously shown to be positive by culture. In addition, PCR detected B virus in several swabs from infected monkeys that were culture negative. Total DNA extracts from the trigeminal and sacral ganglia of these animals were tested by nested PCR and B virus DNA was detected in the trigeminal ganglia of 3 of the 4 orally-infected cynomolgus monkeys. Nested PCR did not detect B virus DNA in total DNA extracts obtained from the brains of the four monkeys.

Keywords

Polymerase Chain Reaction Oligomer Herpes Simplex Herpes Simplex Virus Type Clinical Specimen 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • M. J. Slomka
    • 1
  • D. W. G. Brown
    • 1
  • J. P. Clewley
    • 1
  • A. M. Bennett
    • 2
  • L. Harrington
    • 2
  • D. C. Kelly
    • 2
  1. 1.Virus Reference DivisionCentral Public Health LaboratoryLondon
  2. 2.Chemical and Biological Defence EstablishmentSalisburyU.K.

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