Archives of Virology

, Volume 84, Issue 3–4, pp 181–195 | Cite as

Characterization of a new calicivirus isolated from feces of a dog

  • F. L. Schaffer
  • M. E. Soergel
  • J. W. Black
  • D. E. Skilling
  • A. W. Smith
  • W. D. Cubitt
Original Papers


Canine calicivirus (CaCV), isolated from feces of a dog with diarrhea, was readily propagated in cultures of canine cells and in a dolphin cell line. Serologic evidence indicated many dogs in at least one geographic area had been infected with CaCV, but its role as an etiologic agent of disease was not established. In cell culture most CaCV virions were strongly cell-associated making purification difficult. CaCV was established as a member of the Caliciviridae by morphology and physicochemical properties of virions (density, sedimentation rate, single major polypeptide, RNA genome size), although some of the properties differed slightly from those of previously described caliciviruses; evidence was also obtained for caliciviral RNA species in infected cells. Based on tests with antisera to numerous caliciviruses and presumed caliciviruses, CaCV appeared to be not closely related to any previously described virus except the stunting syndrome agent of chickens.


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Copyright information

© Springer-Verlag 1985

Authors and Affiliations

  • F. L. Schaffer
    • 1
  • M. E. Soergel
    • 1
  • J. W. Black
    • 2
  • D. E. Skilling
    • 3
  • A. W. Smith
    • 3
  • W. D. Cubitt
    • 4
  1. 1.Naval Biosciences LaboratoryUniversity of California, School of Public HealthBerkeleyUSA
  2. 2.Specialized Assays, Inc.NashvilleUSA
  3. 3.College of Veterinary MedicineOregon State UniversityCorvallisUSA
  4. 4.Public Health Laboratory, Department of MicrobiologyCentral Middlesex HospitalLondonU.K.

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