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Effect of extra- and intra-mitochondrial calcium on citrulline synthesis

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Summary

A quantitative relationship between rat liver mitochondrial matrix free Ca2+ ([Ca2+]m) and citrullinogenesis has been observed. Maximum citrulline synthesis occurred at 100 to 200nM [Ca2+]m; higher [Ca2+]m caused inhibition. When [Ca2+]m was decreased to below 50nM, by addition of A23187 and EGTA, inhibition also occurred. By incubating mitochondria with ruthenium red ([Ca2+]m = 200nM) prior to addition of extramitochondrial free Ca2+ ([Ca2+]o) it was found that high external Ca2+ (800nM) did not inhibit citrulline synthesis thus demonstrating that [Ca2+]m, not [Ca2+]o was controlling citrullinogenesis.

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Authors and Affiliations

  1. Instituto de Investigaciones Químico-Biológicas, Universidad Michoacana de San Nicolás de Hidalgo, edificio B-1 C.U. Morelia, 58030, Mich., México

    A. Saavedra-Molina

  2. Department of Biological Chemistry, Hahnemann University School of Medicine, Philadelphia, Pennsylvania, USA

    T. M. Devlin

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  1. A. Saavedra-Molina
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  2. T. M. Devlin
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Saavedra-Molina, A., Devlin, T.M. Effect of extra- and intra-mitochondrial calcium on citrulline synthesis. Amino Acids 12, 293–298 (1997). https://doi.org/10.1007/BF01373009

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  • DOI: https://doi.org/10.1007/BF01373009

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