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International Journal of Legal Medicine

, Volume 104, Issue 5, pp 247–250 | Cite as

Sex determination and species exclusion in forensic samples with probe cY97

  • Paolo Fattorinil
  • Simone Cacció
  • Stefano Gustincich
  • Jonathan Wolfe
  • Bruno Maria Altamura
  • Giorgio Graziosil
Original Articles

Summary

A total of 120 human samples of blood, saliva and semen stains, hair roots, bone and skin fragments, obtained from 30 males and 16 females were analyzed in Southern blots with probe cY97. Only the male samples gave a specific band of 5.7 kb. In dot blot, under high stringency conditions, male DNA gave signals equivalent to a quantity of female DNA eight times higher. Probe cY97 did not react with 9 different vertebrate species but gave a signal for monkey DNA when used at low stringency. The advantage of using a probe specific for the centromeric region for sex determination and species exclusion is discussed.

Key words

Sex identification Species exclusion Southern blot Dot blot cY97 

Zusammenfassung

Insgesamt wurden 120 menschliche Blutproben, Speichelproben und Spermaspuren, Haarwurzeln, Knochen und Hautpartikel, welche von 30 männlichen und 16 weiblichen Personen erhalten wurden, in der Southern-Blot-Technik analysiert mit Hilfe der Probe cY97. Nur die männlichen Proben gaben eine spezifische Bande von 5,7 Kb. Im Dot-Blot, unter hochstringenten Bedingungen, ergab die männliche DNA Signale, welche einer 8-fach höheren Menge von weiblicher DNA entsprachen. Die Proben cY97 reagierte nicht mit 9 verschiedenen Wirbeltierspezies, aber sie ergab ein Signal für Affen-DNA, wenn unter geringen Stringenzbedingungen hybridisiert wurde. Der Vorteil des Gebrauchs einer Probe zur Geschlechtsbestimmung und zum Speziesausschluß, welche spezifisch für die Centromer-Region ist, wird diskutiert.

Schüsselwörter

Geschlechtsbestimmung Speziesausschluß Southern-Blot Dot-Blot cY97 

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Copyright information

© ger-Verlag 1991

Authors and Affiliations

  • Paolo Fattorinil
    • 1
  • Simone Cacció
    • 1
  • Stefano Gustincich
    • 1
  • Jonathan Wolfe
    • 2
  • Bruno Maria Altamura
    • 3
  • Giorgio Graziosil
    • 1
  1. 1.Laboratorio Genetica, Dipartimento di BiologiaUniversitäTriesteItaly
  2. 2.Galton LaboratoriesUniversity CollegeUK
  3. 3.Istituto di Medicina Legale, UniversitäTriesteItaly

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