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Overexpression of Gsα subunit in thyroid tumors bearing a mutated Gsα gene

  • Victor N. Gorelov
  • Kristoffel Dumon
  • Natalie S. Barteneva
  • Dieter Palm
  • Hans-Dieter Röher
  • Peter E. Goretzki
Original Paper Experimental Oncology

Abstract

Point mutations occurring at codon 201 of the gene coding for the α subunit of the stimulatory G protein impair intrinsic GTPase activity and lead to a constitutive activation of adenylate cyclase. We have examined thyroid follicular and papillary carcinomas and follicular adenomas and found samples that bear this mutation at codon 201 of the Gsα gene. Both mutation-positive and mutation-negative tissue samples were investigated for the level of Gsα expression relative to a pool of normal thyroid tissue, using immunoblotting against two (mid-region-specific and C-end-specific) antipeptide antibodies. Using 8000g and 100 000g membrane fractions of homogenized tissues we have demonstrated that the Gsα proteins in normal ad neoplastic thyroid tissues are represented by three isoforms: 43 kDa, 45 kDa and 52 kDa. We have quantified and compared the amount of Gsα protein and find it is overexpressed in mutation-bearing tissue samples.

Key words

Gsα gene Expression Point mutation Immunoblotting Thyroid tumors 

Abbreviations

Gs protein

Stimulatory heterotrimeric guanine-nucleotide-binding protein

BCIP

5-bromo-4-chloro-3-indoxyl phosphate

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Victor N. Gorelov
    • 1
  • Kristoffel Dumon
    • 1
  • Natalie S. Barteneva
    • 1
  • Dieter Palm
    • 2
  • Hans-Dieter Röher
    • 1
  • Peter E. Goretzki
    • 1
  1. 1.Department of Surgery AHeinrich-Heine-University, Gebäude 23.12.01/Raum 81, Düsseldorf UniversitätDüsseldorfGermany
  2. 2.Department of BiochemistryUniversity of WürzburgGermany

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