, Volume 146, Issue 1, pp 28–34 | Cite as

Microtubule organization in the sperm ofTradescantia virginiana

  • B. A. Palevitz
  • M. Cresti


Microtubules were visualized in the sperm ofTradescantia virginiana pollen tubes grownin vitro and processed for antitubulin immunocytochemistry. The sperm contain thick microtubule bundles from which emerge numerous branches of various dimensions disposed longitudinally and helically along the cell axis. Sperm are usually spindle or cigar-shaped, but cells of various sizes and shapes can be found. All contain microtubule arrays. No F-actin was detected in sperm using rhodamine-phalloidin staining. Sperm microtubules are discussed in terms of their potential roles in cell shaping and motility and their origin during generative cell division.


Generative cell Microtubule Sperm Pollen Tradescantia 





immunoglobulin G


Mascarenhas and Walker medium






phosphate-buffered saline


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  1. Burgess J (1970) Cell shape and mitotic spindle formation in the generative cell ofEndymion non-scriptus. Planta 95: 72–85Google Scholar
  2. Cass DD (1973) An ultrastructural and Nomarski-interference study of the sperms of barley. Can J Bot 51: 601–605.Google Scholar
  3. —,Karas I (1975) Development of sperm cells in barley. Can J Bot 53: 1051–1062Google Scholar
  4. Coleman AW, Goff L (1985) Applications of fluorochromes to pollen biology. I. Mithramycin and 4′,6-diamidino-2-phenylindole (DAPI) as vital stains and for quantitation of nuclear DNA. Stain Tech 60: 145–154Google Scholar
  5. Cresti M, Ciampolini F, Kapil RN (1984) Generative cells of some angiosperms with particular emphasis on their microtubules. J Submicrosc Cytol 16: 317–326Google Scholar
  6. —,Ciampolini F, Tiezzi A (1986) Ultrastructural studies onNicotiana tabacum pollen tubes grown in different culture medium (preliminary results). Acta Bot Neerl 35: 285–292Google Scholar
  7. Derksen J, Pierson ES, Traas JA (1985) Microtubules in vegetative and generative cells of pollen tubes. Eur J Cell Biol 38: 142–148Google Scholar
  8. Dumas C, Knox RB, Gaude T (1985) The spatial association of the sperm cells and vegetative nucleus in the pollen grain ofBrassica. Protoplasma 124: 168–174Google Scholar
  9. Dupuis I, Roeckel P, Matthys-Rochon E, Dumas C (1987) Procedure to isolate viable sperm cells from corn (Zea mays L) pollen grains. Plant Physiol 85: 876–878Google Scholar
  10. Heslop-Harrison J, Heslop-Harrison Y (1984) The disposition of gamete and vegetative-cell nuclei in the extending pollen tubes of a grass species,Alopecurus pratensis L. Acta Bot Neerl 33, 131–134Google Scholar
  11. —,Heslop-Harrison Y (1987) An analysis of gamete and organelle movement in the pollen tube ofSecale cereale L. Plant Sci 51: 203–213Google Scholar
  12. Hoefert LL (1969) Fine structure of sperm cells in pollen grains ofBeta. Protoplasma 68: 237–240Google Scholar
  13. Karas I, Cass DD (1976) Ultrastructural aspects of sperm cell formation in rye: evidence for cell plate involvement in generative cell division. Phytomorphology 26: 36–45Google Scholar
  14. Lafleur GJ, Gross AE, Mascarenhas JP (1981) Optimization of culture conditions for the formation of sperm cells in pollen tubes ofTradescantia. Gamete Res 4: 35–40Google Scholar
  15. Lancelle SA, Cresti M, Hepler PK (1987) Ultrastructure of the cytoskeleton in freeze-substituted pollen tubes ofNicotiana alata. Protoplasma 140: 141–150Google Scholar
  16. Matthys-Rochon E, Vergne P, Detchepare S, Dumas C (1987) Male germ unit isolation from three tricellular pollen species:Brassica oleracea, Zea mays andTriticum aestivum. Plant Physiol 83: 464–466Google Scholar
  17. McConchie CA, Hough T, Knox RB (1987) Ultrastructural analyses of the sperm cells of mature pollen of maize,Zea mays. Protoplasma 139: 9–19Google Scholar
  18. Mogensen HL (1986) On the male germ unit in an angiosperm with bicellular pollen,Hippeastrum vitatum. In:Mulcahy DL, Mulcahy GB, Ottaviano E (eds) Biotechnology and ecology of pollen. Springer, Berlin Heidelberg New York Tokyo, pp 297–305Google Scholar
  19. —,Wagner VT (1987) Associations among components of the male germ unit followingin vivo pollination in barley. Protoplasma 138: 161–172Google Scholar
  20. Palevitz BA (1987 a) Actin in the preprophase band ofAllium cepa. J Cell Biol 104: 1515–1519Google Scholar
  21. — (1987 b) The accumulation of F-actin during cytokinesis inAllium. Correlation with microtubule distribution and the effects of drugs. Protoplasma 141: 24–32Google Scholar
  22. — (1988) Cytochalasin-induced reorganization of actin inAllium root cells. Cell Motil Cytoskel 9: 283–298Google Scholar
  23. Perdue TD, Parthasarathy MV (1985) In situ localization of Factin in pollen tubes. Eur J Cell Biol 39: 13–20Google Scholar
  24. Pierson ES, Derksen J, Traas JA (1986) Organization of microfilaments and microtubules in pollen tubes grownin vitro orin vivo in various angiosperms. Eur J Cell Biol 41: 14–18Google Scholar
  25. Russell SD (1984) Ultrastructure of the sperm ofPlumbago zeylanica. II. Quantitative cytology and three-dimensional organization. Planta 162: 385–391Google Scholar
  26. — (1985) Preferential fertilization inPlumbago: Ultrastructural evidence for gamete-level recognition in an angiosperm. Proc Natl Acad Sci USA 82: 6129–6132Google Scholar
  27. — (1986 a) Isolation of sperm cells from the pollen ofPlumbago zeylanica. Plant Physiol 81: 317–319Google Scholar
  28. — (1986 b) Dimorphic sperm, cytoplasmic transmission and preferential fertilization inPlumbago zeylanica. In:Mantell SH, Chapman GP, Street P (eds) The chondriome. Longman Group, London, pp 69–116Google Scholar
  29. —,Cass DD (1981) Ultrastructure of the sperms ofPlumbago zeylanica. I. Cytology and association with the vegetative nucleus. Protopolasma 107: 85–107Google Scholar
  30. Sanger JM, Jackson WT (1971) Fine structure study of pollen development inHaemanthus katherinae Baker. II. Microtubules and elongation of the generative cells. J Cell Sci 8: 303–315Google Scholar
  31. Tiezzi A, Cresti M, Ciampolini F (1986) Microtubules inNicotiana pollen tubes: ultrastructural, immunofluorescence and biochemical data. In:Cresti M, Dallai R (eds) Biology of reproduction and cell motility in plants and animals. University of Siena, Siena, pp 87–94Google Scholar
  32. Valnes K, Brandtzaeg P (1985) Retardation of immunofluorescence fading during microscopy. J Histochem Cytochem 33: 755–761Google Scholar
  33. Wylie RB (1923) Sperm ofVallisneria spiralis. Bot Gaz 75: 191–201Google Scholar
  34. Zhou C, Orndorff K, Allen RD, DeMaggio AE (1986) Direct observations on generative cells isolated from pollen grains ofHaemanthus katherinae Baker. Plant Cell Rep 5: 306–309Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • B. A. Palevitz
    • 1
  • M. Cresti
    • 2
  1. 1.Department of BotanyUniversity of GeorgiaAthens
  2. 2.Dipartimento di Biologia AmbientaleUniversitá di SienaSiena

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