Protoplasma

, Volume 146, Issue 1, pp 18–27

Structure and function of the microtubular cytoskeleton during endosperm development in wheat: an immunofluorescence study

  • A. A. M. Van Lammeren
Article

DOI: 10.1007/BF01354291

Cite this article as:
Van Lammeren, A.A.M. Protoplasma (1988) 146: 18. doi:10.1007/BF01354291

Summary

The three-dimensional structure of the microtubular cytoskeleton of developing wheat endosperm was investigated immunocytochemically. Semi-thin sections were prepared from polyethylene glycol embedded ovaries. At the free-nuclear stage the endosperm cytoplasm with regularly distributed nuclei surrounded a large central vacuole and exhibited an extensive network of fluorescent labelled microtubular assemblies radiating from each nucleus. As was found in other coenocytes, this particular and nuclear-dependent cytoskeletal configuration functions in the arrangement of nuclei and in the stabilization of the nuclear positions. At the beginning of cellularization of the endosperm the formation of vacuoles altered the radiating networks. It is likely that the radiating microtubular arrays function in the formation of phragmoplasts, independent of nuclear divisions. The formation of anticlinal cell walls, giving rise to openended cell cylinders, coincides with the occurrence of phragmoplast microtubular arrays which were demonstrated during the period of cell wall elongation. The microtubular system radiating from the nuclei in these cell cylinders anchored the nuclei in stage- and locus-specific positions. During the development of aleurone and inner endosperm cells, cell morphogenesis was related to earlier demonstrated types of microtubular configurations in the cortical cytoplasm. This suggests that a general mechanism is involved.

Keywords

Cytoskeleton Endosperm Immunofluorescence Microtubular assemblies Polyethylene glycol Triticum Wheat 

Abbreviations

A

alveolus

AL

aleurone layer

CE

central endosperm

CV

central vacuole

DAP

days after pollination

END

endosperm

FITC

fluorescein isothiocyanate

GAR-FITC

goat anti-rabbit antibodies conjugated with FITC

I

integument

IE PC

inner epidermis pericarp

II

inner integument

N

nucleus

NC

nucellus cells

NE

nucellar epidermis

NUC

nucellus

OI

outer integument

PBS

phosphate buffered saline

PC

pericarp

PEG

polyethylene glycol

V

vacuole

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • A. A. M. Van Lammeren
    • 1
  1. 1.Department of Plant Cytology and MorphologyAgricultural University WageningenWageningen

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