, Volume 158, Issue 1–2, pp 45–52 | Cite as

Nuclear size, DNA content, and chromatin condensation are different in individual tissues of the maize root apex

  • F. Baluška
Original Papers


Nuclei of various tissues exhibit different structure in the maize root apex. Moreover, the nuclear structure is in close correlation with the DNA and RNA synthesis. These observations are in contradiction with the hypothesis according to which the chromatin organization in plant nuclei is species-specific and does not correspond to the metabolic activity of nuclei. The possible reasons for this disagreement are discussed.

The extended state of chromatin is not the passive result of synthetic processes in the nucleus, but, on the contrary, it is one of the primary factors which are indispensable for the DNA transcription. Results presented here together with data from literature suggest that the organization of chromatin complex appears as a general control mechanism which determines the efficiency of other more specific mechanisms.


Chromatin condensation DNA content Nuclear size Root apex Root tissues Zea mays


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  1. Baluška F (1990) Influence of developing root primordia on nuclei of neighbouring tissues inZea mays L. primary root. Biológia (Bratislava) (in press)Google Scholar
  2. — (1989) Chromatin structure in various tissues of the primary root ofZea mays L. In: Loughman BC, Gašparíková O, Kolek J (eds) Structural and functional aspects of transport in roots. Kluwer, Dordrecht, pp 49–52Google Scholar
  3. —, Kubica Š (1984) Changes in DNA content and chromatin condensation in relation to growth processes during germination of wheat. Biológia (Bratislava) 39: 1059–1066Google Scholar
  4. — — (1987 a) Changes in chromatin condensation during growth and differentiation of maize primary root cells. Biológia (Bratislava) 42: 9–16Google Scholar
  5. — — (1987 b) DNA content, nucleus size and chromatin structure in cells of the maize primary root. Biológia (Bratislava) 42: 409–417Google Scholar
  6. — —, Hauskrecht M (1990) Postmitotic “isodiametric” cell growth in the maize root apex. Planta 181: 269–274Google Scholar
  7. Bansal J, Davidson D (1978) Heterogeneity of meristematic cells ofVicia faba: evidence from nuclear and chromosome volumes and from nuclear protein content. Caryologia 31: 161–177Google Scholar
  8. Barlow PW (1971) Properties of the cells in the root apex. Rev Fac Agronom La Plata 47: 275–301Google Scholar
  9. — (1977) Determinants of nuclear chromatin structure in angiosperms. Ann Sci Nat Bot [Ser 12] 18: 193–205Google Scholar
  10. — (1978) RNA metabolism in the quiescent centre and neighbouring cells in the root meristem ofZea mays. Z Pflanzenphysiol 86: 147–157Google Scholar
  11. — (1985) Nuclear chromatin structure in relation to cell differentiation and cell activation in the cap and quiescent centre ofZea mays L. J Exp Biol 36: 1492–1503Google Scholar
  12. — (1989) Meristems, metamers and modules and the development of shoot and root system. Bot J Linn Soc 100: 255–279Google Scholar
  13. Bodnar JW (1988) A domain model for eukaryotic DNA organization: a molecular basis for cell differentiation and chromosome evolution. J Theor Biol 132: 479–507Google Scholar
  14. Bouvier-Durand M, Real M, Come D (1989) Changes in nuclear activity upon secondary dormancy induction by abscisic acid in apple embryo. Plant Physiol Biochem 27: 511–518Google Scholar
  15. Cionini PG, Cremonini R, Bassi P (1984) Highly repeated DNA sequences and the structural organization of plant cell nuclei. Ann Bot (Roma) 42: 29–44Google Scholar
  16. Davidson D, Golding BG, Armstrong SW (1978) Increases in nuclear volume and cell size in meristematic cells arrested by 5-aminouracil. Protoplasma 96: 47–57Google Scholar
  17. Deltour R (1985) Nuclear activation during early germination of the higher plant embryo. J Cell Sci 75: 43–83Google Scholar
  18. Greilhuber J (1988) “Self-tanning”-a new and important source of stoichiometric error in cytophotometric determination of nuclear DNA content in plants. Pl Syst Evol 158: 87–96Google Scholar
  19. Guerri J, Culianez F, Primo-Millo E, Primo-Yufera E (1982) Chromatin changes related to dedifferentiation and differentiation in tobacco tissue culture (Nicotiana tabacum L.). Planta 155: 273–280Google Scholar
  20. Havelange A, Jeanny JC (1984) Changes in density of chromatin in the meristematic cells ofSinapis alba during transition to flowering. Protoplasma 122: 222–232Google Scholar
  21. Koleva ST, Marinova ET, Dimitrov BD, Tourishcheva MS (1989) Investigation of histone proteins in plant nuclei possessing different ultrastructural organization. Protoplasma: 150: 96–102Google Scholar
  22. Kubica Š (1984) Chromatin condensation during cell cycles of differentiating barley root central metaxylem. Biológia (Bratislava) 39: 1067–1073Google Scholar
  23. —, Baluška F, Gašparíková O (1989) Pattern of nucleic acids synthesis in the root apex ofZea mays L. Biológia (Bratislava) 44: 201–207Google Scholar
  24. Luxová M (1980) Kinetics of maize root growth. Ukr J Bot 37: 68–72 (in Ukr.)Google Scholar
  25. Lyndon RF (1967) The growth of the nucleolus in dividing and nondividing cells of the pea root. Ann Bot 31: 133–146Google Scholar
  26. Medina F-J, Solanilla EL, Sanchez-Pina MA, Fernandez-Gomez ME, Risueno MC (1986) Cytological approach to the nucleolar functions detected by silver staining. Chromosoma 94: 259–266Google Scholar
  27. Nagl W (1970) The mitotic and endomitotic nuclear cycle inAllium carinatum. II. Relations between DNA replication and chromatin structure. Carylogia 23: 71–78Google Scholar
  28. — (1979) Nuclear ultrastructure: condensed chromatin in plants is species-specific (karyotypical), but not tissue-specific (functional). Protoplasma 100; 53–71Google Scholar
  29. —, Cabriol H, Lahr C, Greulach H, Ohlinger H-M (1983) Nuclear ultrastructure: morphometry of nuclei from various tissues ofCucurbita, Melandrium, Phaseolus, Tradescantia andVicia. Protoplasma 115: 59–64Google Scholar
  30. Rembur J, Nougarede A (1987) Microspectrophotometric measurements of DNA by automated computerized scanning in cycling cells of theChrysanthemum segetum shoot apex. Protoplasma 136: 183–190Google Scholar
  31. Rosenberg O (1904) Über die Individualität der Chromosomen im Pflanzenreich. Flora 93: 251Google Scholar
  32. Saugy M, Pilet PE (1984) Endogenous indol-3yl-acetic acid in the stele and cortex of gravistimulated maize roots. Plant Sci Lett 37: 93–99Google Scholar
  33. Sauter JJ, Ulrich H (1977) Cytophotometric investigation of DNA and RNA content in nuclei of active Strasburger cells inPinus nigra var.austriaca (Hoess.) Badoux. Planta 137: 5–11Google Scholar
  34. Sgorbati S, Sparvoli E, Levi M, Chiatante D, Giordano P (1988) Bivariate cytofluorometric analysis of DNA and nuclear protein content in plant tissues. Protoplasma 144: 180–184Google Scholar
  35. Sparrow AH, Price HJ, Underbrink AG (1972) A survey of DNA content per cell and per chromosome of procaryotic and eucaryotic organisms: some evolutionary considerations. Brookhaven Symp Biol 23: 451–494Google Scholar
  36. Spiker S (1985) Plant chromatin structure. Annu Rev Plant Physiol 36: 235–253Google Scholar
  37. Testillano PS, Risueno MC (1988) Evolution of nuclear interchromatin structures during microspore interphase period. In: Cresti M, Gori P, Pacini E (eds) Sexual reproduction in higher plants. Siena, Italy, pp 151–156Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • F. Baluška
    • 1
  1. 1.Institute of BotanySlovak Academy of SciencesBratislavaCSFR

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