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Apoptosis

, Volume 1, Issue 3, pp 209–212 | Cite as

Upregulation of Fas-induced apoptosis by interferon-γ accompanied by increased ICE expression in renal cell cancer cells

  • Y. Tomita
  • V. Bilim
  • M. Takeda
  • K. Takahashi
Reports

Abstract

The integration of Fas/Apo-1 (CD95) by Fas ligand or anti-Fas antibody induces apoptosis, and this system plays a pivotal role for the lysis of target cells by cytotoxic T lymphocytes. Fas-mediated apoptosis is also increased by a prior incubation of Fas-bearing cells with interferon(IFN)-γ. Interleukin-1-β converting enzyme (ICE) and/or CPP32, or other members of ICE family act as direct cell death executors downstream of this mechanism, and a tetrapeptide inhibitor of these cysteine proteases blocks Fas-mediated apoptosis. In this study, we examined the effect of IFN-γ on Fas-mediated apoptosis in ACHN cells. IFN-γ augmented apoptosis in a dose dependent manner and reached a plateau at 400 U/ml when exposed for 48 h before the end of culture. The kinetics revealed a significant increase in apoptosis after 24 h. Exposing ACHN cells to IFN-γ increased pro-ICE expression accompanied with a decrease of pro-CPP32. These results suggest that direct enhancement of ICE expression and/or upregulation of conversion of pro-CPP32 to active form increases Fas-mediated apoptosis by IFN-γ in ACHN cells.

Key words

Apoptosis Fas interferon-γ renal cell cancer 

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Copyright information

© Rapid Science Publishers 1996

Authors and Affiliations

  • Y. Tomita
    • 1
  • V. Bilim
    • 1
  • M. Takeda
    • 1
  • K. Takahashi
    • 1
  1. 1.Department of UrologyNiigata University School of MedicineNiigataJapan

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