Archives of Virology

, Volume 96, Issue 3–4, pp 123–134 | Cite as

Glycosylation, an important modifier of rotavirus antigenicity

  • J. Caust
  • M. L. Dyall-Smith
  • I. Lazdins
  • I. H. Holmes
Original Papers

Summary

Mutants of a non-glycosylated strain of SA 11 rotavirus (clone 28), were selected using a monoclonal antibody directed against the VP 7 protein. These mutants possessed an amino acid substitution at residue 238 of VP 7, whereas mutants of wild type SA 11 selected with the same antibody have previously been shown to contain a substitution at residue 211 (i.e., in the antigenic C region). In both cases the mutations produce new potential glycosylation sites, and these were found to be utilized. The mutations also lead to gross antigenic changes, and these were found to be reversible upon removal of the attached carbohydrate. The results suggest an important role for carbohydrate in influencing the exposure of antigenic determinants of the rotavirus serotype-specific protein, VP 7.

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References

  1. 1.
    Arias CF, Lopez S, Espejo RT (1982) Gene protein products of SA 11 simian rotavirus genome. J Virol 41: 42–50Google Scholar
  2. 2.
    Bastardo JW, McKimm-Breschkin JL, Sonza S, Mercer L, Holmes IH (1981) Preparation and characterization of antisera to electrophoretically purified SA 11 virus polypeptides. Infect Immun 34: 641–647Google Scholar
  3. 3.
    Beards GM, Pilford JN, Thouless ME, Flewett TH (1980) Rotavirus serotypes by serum neutralization. J Med Virol 5: 231–237Google Scholar
  4. 4.
    Both GW, Mattick JS, Bellamy AR (1983) Serotype-specific glycoprotein of simian 11 rotavirus: coding assignment and gene sequence. Proc Natl Acad Sci USA 80: 3091–3095Google Scholar
  5. 5.
    Chan W-K, Penaranda ME, Crawford SE, Estes MK (1986) Two glycoproteins are produced from the rotavirus neutralization gene. Virology 151: 243–252Google Scholar
  6. 6.
    Cohen J, Maget-Dana R, Roche AC, Monsigny M (1978) Calf rotavirus: detection of outer capsid glycoproteins by lectins. FEBS Lett 87: 26–29Google Scholar
  7. 7.
    Dyall-Smith ML, Azad AA, Holmes IH (1983) Gene mapping of rotavirus double-stranded RNA segments by Northern blot hybridization: application to segments 7, 8 and 9. J Virol 46: 317–320Google Scholar
  8. 8.
    Dyall-Smith ML, Holmes IH (1984) Sequence homology between human and animal rotavirus serotype-specific glycoproteins. Nucleic Acids Res 12: 3973–3981Google Scholar
  9. 9.
    Dyall-Smith ML, Lazdins I, Tregear GW, Holmes IH (1986) Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proc Natl Acad Sci USA 88: 3465–3468Google Scholar
  10. 10.
    Dyall-Smith ML, Holmes IH (1981) Gene-coding assignments of rotavirus double-stranded RNA segments 10 and 11. J Virol 38: 1099–1103Google Scholar
  11. 11.
    Elbein AD (1981) The tunicamycins-useful tools for studies on glycoproteins. Trends Biochem Sci 6: 219–221Google Scholar
  12. 12.
    Ericson BL, Graham DY, Mason BB, Estes MK (1982) Identification, synthesis, and modifications of simian rotavirus SA 11 polypeptides in infected cells. J Virol 42: 825–839Google Scholar
  13. 13.
    Ericson BL, Graham DY, Mason BB, Hanssen HH, Estes MK (1983) Two types of glycoprotein precursors are produced by the simian rotavirus SA 11. Virology 127: 320–332Google Scholar
  14. 14.
    Estes MK, Graham DY, Ramig RF, Ericson BL (1982) Heterogeneity in the structural glycoprotein (VP 7) of simian rotavirus SA 11. Virology 122: 8–14Google Scholar
  15. 15.
    Graham DY, Estes MK, Hanssen HH, Mason BB, Ericson BL (1983) Studies on VP 7: the structural glycoprotein of the simian rotavirus SA 11. In: Compans RW, Bishop DHL (eds) Double-stranded RNA viruses. Elsevier Press, New York, pp 137–144Google Scholar
  16. 16.
    Kalica AR, Greenberg HB, Wyatt RG, Flores J, Sereno MM, Kapikian AZ, Chanock RM (1981) Genes of human (strain Wa) and bovine (strain UK) rotaviruses that code for neutralization and subgroup antigens. Virology 112: 385–390Google Scholar
  17. 17.
    Kantharidis P, Dyall-Smith ML, Holmes IH (1987) Marked sequence variation between segment 4 genes of human RV-5 and simian SA 11 rotaviruses. Arch Virol 93: 111–121Google Scholar
  18. 18.
    Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685Google Scholar
  19. 19.
    Lazdins I, Sonza S, Dyall-Smith ML, Coulson BS, Holmes IH (1985) Demonstration of an immunodominant neutralization site by analysis of antigenic variants of SA 11 rotavirus. J Virol 56: 317–324Google Scholar
  20. 20.
    Nakagomi O, Kutsuzawa-Nakagomi T, Suto T, Konno T, Ishida N (1983) Glycoprotein of human rotavirus Wa strain grown in MA 104 cells. Tohoku J Exp Med 139: 423–424Google Scholar
  21. 21.
    Petrie BL (1983) Biologic activity of rotavirus particles lacking glycosylated proteins. In: Compans RW, Bishop DHL (eds) Double-stranded RNA viruses. Elsevier Press, New York, pp 145–156Google Scholar
  22. 22.
    Rautenberg P, Reinwald E, Risse HJ (1980) Evidence for concanavalin A binding sites on the surface coat ofTrypanosoma congolense. Parasitology 80: 113–122Google Scholar
  23. 23.
    Skehel JJ, Stevens DJ, Daniels RS, Douglas AR, Knossow M, Wilson IA (1984) A carbohydrate side-chain on hemagglutinin of Hong Kong influenza viruses inhibits recognition by a monoclonal antibody. Proc Natl Acad Sci USA 81: 1779–1783Google Scholar
  24. 24.
    Smith ML, Lazdins I, Holmes IH (1980) Coding assignments of double-stranded RNA segments of SA 11 rotavirus established by in vitro translation. J Virol 33: 976–982Google Scholar
  25. 25.
    Sonza S, Breschkin AM, Holmes IH (1983) Derivation of neutralizing monoclonal antibodies against rotavirus. J Virol 45: 1143–1146Google Scholar
  26. 26.
    Svensson L (1984) Identification of an outer capsid glycoprotein of human rotavirus by concanavalin A. J Gen Virol 65: 2183–2190Google Scholar
  27. 27.
    Thouless ME, Beards GM, Flewett TH (1982) Serotyping and subtyping of rotavirus strains by the ELISA test. Arch Virol 73: 219–230Google Scholar
  28. 28.
    Wilson ID, Skehel JJ, Wiley DC (1981) Structure of the haemagglutinin membrane glycoprotein of influenza virus at 3 Å resolution. Nature 289: 366–373Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • J. Caust
    • 1
  • M. L. Dyall-Smith
    • 1
  • I. Lazdins
    • 1
  • I. H. Holmes
    • 1
  1. 1.School of MicrobiologyUniversity of MelbourneParkvilleAustralia

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