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Archives of Virology

, Volume 74, Issue 1, pp 1–9 | Cite as

Monoclonal antibodies against foot-and-mouth disease virus 146S and 12S particles

  • K. C. McCullough
  • R. Butcher
Original Papers

Summary

This paper is the first description of monoclonal antibodies specific for foot-and-mouth disease virus (FMDV). Using these antibodies, it was possible to distinguish similar and unique antigenic sites on complete (146S) or subunit (12S) virus particles. Some of these monoclonal antibodies could also distinguish between 12S subunits made by acid degradation of 146S virus (12SA) and those viral components found in infected cell lysates which sediment in sucrose density gradients as “12S subunits” (12 SN).

Keywords

Sucrose Monoclonal Antibody Infectious Disease Infected Cell Density Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Adam, K.-H., Kaaden, O. R., Strohmaier, K.: Isolation of immunizing cyanogen bromide peptides of foot-and-mouth disease virus. Biochem. Biophys. Res. Comm.84, 677–683 (1978).Google Scholar
  2. 2.
    Bachrach, H. L.: The determination of the sedimentation constant of a homogeneous component having the characteristics of the foot-and-mouth disease virus. Amer. J. Vet. Res.13, 13–16 (1952).Google Scholar
  3. 3.
    Brown, F., Cartwright, B.: Purification of radioactive foot-and-mouth disease virus. Nature London199, 1168–1170 (1963).Google Scholar
  4. 4.
    Brown, F., Smale, C. J.: Demonstration of three specific sites on the surface of foot-and-mouth disease virus by antibody complexing. J. gen. Virol.7, 115–127 (1970).Google Scholar
  5. 5.
    Burroughs, J. N., Rowlands, D. J., Sangar, D. V., Talbot, P., Brown, F.: Further evidence for multiple proteins in the foot-and-mouth disease virus particle. J. gen. Virol.13, 73–84 (1971).Google Scholar
  6. 6.
    Ceglowski, W. S.: Antibody response to the noninfectious 7 µm component of the virus of foot-and-mouth disease. Virology25, 328–330 (1965).Google Scholar
  7. 7.
    Crowther, J. R., Abu el Zein, E. M. E.: Detection and quantification of foot-and-mouth disease virus by enzyme labelled immunosorbent assay techniques. J. gen. Virol.42, 597–602 (1979).Google Scholar
  8. 8.
    Ferguson, M., Schild, G. C., Minor, P. D., Yates, P. J., Splitz, M.: A hybridoma cell line secreting antibody to poliovirus type 3 D-antigen: detection in virus harvest of two D-antigen populations. J. Virol.54, 437–442 (1981).Google Scholar
  9. 9.
    France, R., Adam, K-H., Strohmaier, K.: Localization and characterization of two immunogenic regions on the coat protein VpThr of foot-and-mouth disease virus (FMDV), subtype 01K, inducing neutralising antibodies. Med. Micro. Immun.169, 154–164 (1981).Google Scholar
  10. 10.
    Giraudon, P., Wild, T. F.: Monoclonal antibodies against measles virus. J. gen. Virol.54, 325–332 (1981).Google Scholar
  11. 11.
    Hardy, M. M., Moore, D. M.: Neutralisation of foot-and-mouth disease virus. 1. Sensitization of the 140S virion by antibody also reactive with the 12S protein subunit. J. gen. Virol.55, 415–427 (1981).Google Scholar
  12. 12.
    Humphrey, D. D., Feorino, P. M.: Mouse hybridoma cell cultures produce antibody to poliovirus type 1. Abst. Ann. Amer. Soc. Microbiol.81, T62 (1981).Google Scholar
  13. 13.
    Kaaden, O. R., Adam, K. H., Strohmaier, K.: Induction of neutralising antibodies and immunity in vaccinated guinea pigs by cyanogen bromide-peptides of VP3. J. gen. Virol.34, 397–400 (1977).Google Scholar
  14. 14.
    Kleid, D. G., Yansura, D., Small, B., Dowbenko, D., Moore, D. M., Grubman, M. J., McKercher, P. D., Morgan, D. O., Robertson, B. H., Bachrach, H. L.: Cloned viral protein vaccines for foot-and-mouth disease: responses in cattle and swine. Science214, 1125–1129 (1981).Google Scholar
  15. 15.
    Knauert, F. K., Milstein, J. B.: Monoclonal antibody specific for the D-antigenic form of poliovirus. Abs. Ann. Mtg. Amer. Soc. Microbiol.81, T 126 (1981).Google Scholar
  16. 16.
    Köhler, G., Milstein, C.: Continuous culture of fused cells secreting antibody of predefined specificity. Nature256, 495–497 (1975).Google Scholar
  17. 17.
    Koprowski, H., Gerhard, W., Croce, C. M.: Production of antibodies against influenza virus by somatic cell hybrids between mouse myeloma and primed spleen cells. Proc. Natl. Acad. Sci. U.S.A.74, 2985–2988 (1977).Google Scholar
  18. 18.
    McFarlin, D. E., Bellin, W. J., Mingioli, E. S., Behar, T. N., Trudgett, A.: Monospecific antibody to the haemagglutinin of measles virus. J. gen. Virol.48, 425–429 (1980).Google Scholar
  19. 19.
    McMaster, W. R., Williams, A. F.: Identification of Ia glycoproteins in rat thymus and purification from rat spleen. Euro. J. Immun.9, 426–433 (1979).Google Scholar
  20. 20.
    Meloen, R. H., Briaire, J.: A study of cross-reacting antigens in the intact foot-and-mouth disease virus and its 12S subunits with antisera against structural proteins. J. gen. Virol.51, 107–116 (1980).Google Scholar
  21. 21.
    Meloen, R. H., Rowlands, D. J., Brown, F.: Comparison of the antibodies elicited by the individual structural polypeptides of foot-and-mouth disease and polio viruses. J. gen. Virol.45. 761–763 (1979).Google Scholar
  22. 22.
    Nabholz, M.: Production and maintenance of antibody secreting myeloma hybrids. In: ‘Hybridoma technology with special reference to parasitic diseases.’ Chapter 3, 23–32. UNDP/World Bank/WHO 1979.Google Scholar
  23. 23.
    Nowinski, R. C., Lostrom, M. E., Tam, M. R., Stone, M. R., Burnett, W. N.: The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukaemia viruses. Virology93, 111–126 (1979).Google Scholar
  24. 24.
    Rowlands, D. J., Sangar, D. V., Brown, F.: Relationship of antigenic structure of foot-and-mouth disease virus to the process of infection. J. gen. Virol.13, 85–93 (1971).Google Scholar
  25. 25.
    Rowlands, D. J., Sangar, D. V., Brown, F.: A comparative and serological study of the full and empty particles of foot-and-mouth disease virus. J. gen. Virol.26, 227–238 (1975).Google Scholar
  26. 26.
    Voller, A., Bidwell, D. E., Bartlett, A.: Enzyme immunoassays in diagnostic medicine. Bull. WHO53, 55–65 (1976).Google Scholar
  27. 27.
    Wiktor, T. J., Koprowski, H.: Monoclonal antibodies against rabies virus produced by somatic cell hybridization: detection of antigenic variants. Proc. Natl. Acad. Sci. U.S.A.75, 3938–3942 (1978).Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • K. C. McCullough
    • 1
  • R. Butcher
    • 1
  1. 1.Animal Virus Research InstitutePirbright, WorkingUnited Kingdom

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