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Archives of Virology

, Volume 64, Issue 4, pp 359–374 | Cite as

The association of calf serum with the contamination of BHK21 clone 13 suspension cells by a parvovirus serologically related to the minute virus of mice (MVM)

  • P. F. Nettleton
  • M. M. Rweyemamu
Original Papers

Summary

An investigation of persistent cell deaths of BHK 21 suspension cells during subculturing resulted in the isolation of a viral agent. The agent was isolated from samples of dead cells, cell growth media and 2 batches of calf serum. It was established that the agent was associated with the use of certain batches of calf serum. The isolated virus was found to replicate effectively only in rapidly growing BHK cell cultures. In monolayers it caused the formation of large intranuclear inclusion bodies. The isolate was a strong haemagglutinin; it was stable to ether, chloroform, pH 3 and heating at 56° C. It was shown to be a DNA-virus and by electron-microscopy it was evident as unenveloped spherical, small particles (21 nm in diameter). In sucrose density gradients the virus sedimented as 2 peaks at approximately 114S and 85-92S, and by caesium chloride equilibrium centrifugation, two main peaks at densities of 1.39 and 1.31 g/ml were evident with a minor peak at 1.35. The capsid of the complete virion consisted of 3 polypeptides. The agent has therefore been provisionally designated a member of the Parvoviridae family, genus parvovirus. Serologically it was found to be related to MVM. The isolated parvovirus was inactivated by 0.05 per cent peracetic acid and 0.05 per cent acetylethyleneimine.

Keywords

Caesium Sucrose Density Gradient Peracetic Acid Intranuclear Inclusion Viral Agent 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bachmann, P. E., Hoggan, M. D., Melnick, J. L., Pereira, H. G., Vago, C.: Parvoviridae. Intervirology5, 83–92 (1975).Google Scholar
  2. 2.
    Bachrach, H. L., Moore, D. M., McKercher, P. D., Polatnick, J.: Immune and antibody responses to an isolated capsid protein of foot-and-mouth disease virus. J. Immunol.115, 1636–1641 (1975).Google Scholar
  3. 3.
    Booth, J. C., Pay, T. W. F., Hedger, R. S., Barnett, I. T.: The use of the haemagglutination inhibition test for detecting antibodies to type SAT 2 foot-and-mouth disease viruses in cattle sera. J. Hyg. Camb.74, 115–122 (1975).Google Scholar
  4. 4.
    Briefman, L., Kline, M. A., Hull, R. N.: The virucidal properties of peracetic acid. Amer. J. clin. Pathol.33, 30–33 (1960).Google Scholar
  5. 5.
    Brown, F., Cartwright, B., Stewart, D. L.: The effect of various inactivating agents on the viral and ribonucleic acid infectivities of foot-and-mouth disease virus and on its attachment to susceptible cells. J. gen. Microbiol.31, 179–186 (1963).Google Scholar
  6. 6.
    Capstick, P. B., Garland, A. J., Masters, R. C., Chapman, W. G.: Some functional and morphological alterations occurring during and after the adaptation of BHK21 clone 13 cells to suspension culture. Exp. Cell Res.44, 119–128 (1966).Google Scholar
  7. 7.
    Clinton, G. M., Hayashi, M.: The parvovirus MVM: Particles with altered structural proteins. Virology66, 261–267 (1975).Google Scholar
  8. 8.
    Clinton, G. M., Hayashi, M.: The parvovirus MVM: A comparison of heavy and light particle infectivity and their density conversionin vitro. Virology74, 57–63 (1976).Google Scholar
  9. 9.
    Crawford, L. V.: A minute virus of mice. Virology29, 605–612 (1966).Google Scholar
  10. 10.
    Crawford, L. V., Follett, E. A. C., Burdon, M. G., McGeogh, D. J.: The DNA of a minute virus of mice. J. gen. Virol.4, 37–46 (1969).Google Scholar
  11. 11.
    Croghan, D. L., Matchett, A., Koski, T. A.: Isolation of porcine parvovirus from commercial trypsin. Appl. Microbiol.26, 431–433 (1973).Google Scholar
  12. 12.
    Hallauer, C., Kronauer, G.: Nachweis eines nicht identifizierten Hämagglutinins in menschlichen Tumor-Zellstämmen. Arch. ges. Virusforsch.11, 754–756 (1962).Google Scholar
  13. 13.
    Hallauer, C., Kronauer, G., Siegl, G.: Parvoviruses as contaminants of permanent human cell lines. I. Virus isolation from 1960–1970. Arch. ges. Virusforsch.35, 80–90 (1971).Google Scholar
  14. 14.
    Hallauer, C., Siegl, G., Kronauer, G.: Parvoviruses as contaminants of permanent human cell lines. III. Biological properties of the isolated viruses. Arch. ges. Virusforsch.38, 366–382 (1972).Google Scholar
  15. 15.
    Harris, R. E., Coleman, P. H., Morahan, P. S.: Stability of minute virus of mice to chemical and physical agents. Appl. Microbiol.28, 351–354 (1974).Google Scholar
  16. 16.
    Hoggan, D. M.: In:Maramorosch, K., Kurstak, E. (eds.), Comparative Virology, Ch. 2: Small DNA viruses. New York: Academic Press 1971.Google Scholar
  17. 17.
    Johnson, R. H.: Feline panleucopaenia. I. Identification of a virus associated with the syndrome. Res. vet. Sci.6, 466–471 (1965).Google Scholar
  18. 18.
    Lennette, E. H., Schmidt, N. J.: Diagnostic procedures for viral and rickettsial infections. 4th Edition. Published by American Public Health Association. Inc. 1969.Google Scholar
  19. 19.
    MacPherson, I. A., Stoker, M. G. P.: Polyoma transformation of hamster cell clones—an investigation of genetic factors affecting cell competence. Virology16, 147–151 (1962).Google Scholar
  20. 20.
    Maizel, J. V., jr.: In: Methods in Virology, Vol. 5, 179. New York: Academic Press 1971.Google Scholar
  21. 21.
    Matsunaga, Y., Matsuno, S., Mukoyama, J.: Isolation and characterisation of a parvovirus of rabbits. Infect. Immun.18, 495–500 (1977).Google Scholar
  22. 22.
    Mayr, A., Bachmann, P. A., Siegl, G., Mahnel, H., Shefly, B. E.: Characterisation of a small porcine DNA virus. Arch. ges. Virusforsch.25, 38–51 (1968).Google Scholar
  23. 23.
    Morimoto, T., Fujisaki, Y., Ito, Y., Tanaka, Y.: Biological and physicochemical properties of porcine parvovirus recovered from stillborn piglets. Nat. Inst. Anim. Hlth. Quart.12, 137–144 (1972).Google Scholar
  24. 24.
    Parker, J. C., Collins, M. J., Cross, S. S., Rowe, W. P.: Minute virus of mice. II. Prevalence, epidemiology and occurrence as a contaminant of transplanted tumours. J. Nat. Canc. Inst.45, 305–310 (1970).Google Scholar
  25. 25.
    Rweyemamu, M. M., Loretu, K.: Isolation of “non-syncitia forming” herpes viruses from cattle in Tanzania. J. comp. Pathol.83, 377–386 (1973).Google Scholar
  26. 26.
    Schweizer, H., Sprossiy, M., Mucke, H., Wutzler, P.: Peracetic acid as sterilising agent for culture serum. Nature New Biology240, 61–62 (1972).Google Scholar
  27. 27.
    Shapiro, A. L., Vinuela, E., Maizel, J. V., jr.: Molecular weight estimation of polypeptide chains by electrophoresis in SDS-polyacrylamide gels. Biochem. biophys. Res. Commun.28, 815–820 (1967).Google Scholar
  28. 28.
    Siegl, G.: The parvoviruses. Virology Monographs, Vol. 15. Wien-New York: Springer 1976.Google Scholar
  29. 29.
    Siegl, G., Hallauer, C., Novak, A., Kronauer, G.: Parvoviruses as contaminants of permanent human cell lines. II. Physicochemical properties of the isolated virus. Arch. ges. Virusforsch.35, 91–103 (1971).Google Scholar
  30. 30.
    Studdert, M. J., Peterson, J. E.: Some properties of feline panleukopaenia virus. Arch. ges. Virusforsch.42, 346–354 (1973).Google Scholar
  31. 31.
    Tattersall, P. J., Ward, D. C., Shatkin, A. J.: Sequence overlap between the structural polypeptides of the minute virus of mice. Abstr. Third. Int. Congr. Virology, Madrid, 183, 1975.Google Scholar
  32. 32.
    Tattersall, P. J., Cawte, P. J., Shatkin, A., Ward, D. C.: Three structural polypeptides coded for by minute virus of mice, a parvovirus. J. Virol.20, 273–289 (1976).Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • P. F. Nettleton
    • 1
  • M. M. Rweyemamu
    • 1
  1. 1.The Wellcome Foundation LimitedWellcome Foot-and-Mouth Disease Vaccine LaboratoryPirbright, WorkingEngland

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