Archives of Virology

, Volume 47, Issue 2, pp 147–155 | Cite as

Strain-specific degradation of a viral glycoprotein in Newcastle disease virus-infected cells

  • Elena I. Sklyanskaya
  • N. V. Kaverin
  • Natalia V. Gribkova
  • Inna V. Tsvetkova
  • M. A. Lipkind
Article
Received:

Summary

In cells infected with mesogenic or lentogenic strain of Newcastle disease virus the level of neuraminidase and hemagglutinin activities sharply decreased after the addition of cycloheximide. With two velogenic strains such decreases did not occur.

The infected cells were labelled with14C-amino acids (leucine or valine) and further incubated with an excess of unlabelled precursor. Polyacrylamide gel analysis revealed a decrease of the peak corresponding to the “large” glycoprotein after the chase in cells infected with meso- or lentogenic strain (Beaudette, B1). In the cells infected with velogenic strains (Italia, Herts) no such decrease was observed. The degradation of the “large” glycoprotein as the cause of the decrease of hemagglutinin and neuraminidase activities in cycloheximide-treated cells and its possible relation to virulence is discussed.

Keywords

Polyacrylamide Leucine Infected Cell Meso Valine 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1975

Authors and Affiliations

  • Elena I. Sklyanskaya
    • 1
    • 2
  • N. V. Kaverin
    • 1
    • 2
  • Natalia V. Gribkova
    • 1
    • 2
  • Inna V. Tsvetkova
    • 1
    • 2
  • M. A. Lipkind
    • 1
    • 2
  1. 1.Ivanovsky Institute of Virology, Institute of Biological and Medical ChemistryMoscow
  2. 2.Veterinary AcademyMoscowU.S.S.R.

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