Herpes simplex virus type 1 infection in mice with severe combined immunodeficiency (SCID)
Herpes simplex virus type 1 (HSV-1) infection in mutant mice with severe combined immunodeficiency (SCID mice), i.e., mice in which the differentiation of both T and B lymphocytes is severely impaired, was studied.
All control (infected and not treated with antibodies or with immune spleen cells) SCID mice were dead by 17 days after intracutaneous injection in the right midflank with 1 × 105 PFU of a virulent HSV-1 strain, Hayashida. Immunization with an avirulent strain of HSV-1 (SKa) did not protect them from death or prolong the survival time. Tissue virus titration of infected mice killed at various times after inoculation detected infectious virus in various organs, dorsal root ganglia, spinal cord, brain, kidney and adrenal gland in addition to the inoculation site of the skin in SCID mice, whereas virus could be detected only in the inoculation site and the nervous tissues in euthymic BALB/c mice, and in the adrenal gland from only one out of 17 nude mice.
Human gamma globulin containing neutralizing antibody against HSV-1 prolonged the survival time but did not protect SCID mice from death. Transfer of spleen cells from immunized BALB/c mice protected the infected SCID mice from death. Treatment of spleen cells with anti-Thy 1.2 monoclonal antibody and complement abolished the protection.
KeywordsDorsal Root Ganglion Adrenal Gland Spleen Cell Infected Mouse SCID Mouse
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- 1.Bosma GC, Custer RP, Bosma MJ (1983) A severe combined immunodeficiency mutation in the mouse. Nature 301: 527–530Google Scholar
- 2.Custer RP, Bosma GC, Bosma MJ (1985) Severe combined immunodeficiency (SCID) in the mouse. Pathology, reconstitution, neoplasms. Am J Pathol 120: 464–477Google Scholar
- 3.Hayashi Y, Nagata M, Yoshizumi H, Mori R, Navarro CV, Yang HD (1984) Difference in age distribution of antibody to herpes simplex virus in Fukuoka, Manila and Busan. Japan J Med Sci Biol 37: 35–39Google Scholar
- 4.Hill TJ, Yirrell DL, Blyth WA (1986) Infection of the adrenal gland as a route to the central nervous system after viraemia with herpes simplex virus in the mouse. J Gen Virol 67: 309–320Google Scholar
- 5.Hong R (1983) Combined immunodeficiency disease (CID). In: Behrman RE, Vaughan VC III (ed) Textbook of pediatrics, 12th edn. WB Saunders, Philadelphia, pp 508–509Google Scholar
- 6.Julis MH, Simpson E, Herzenberg LA (1973) A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol 3: 645–662Google Scholar
- 7.Kapoor AK, Nash AA, Wildy P, Phelan J, McLean CS, Field HJ (1982) Pathogenesis of herpes simplex virus in congenitally athymic mice: the relative roles of cell-mediated and humoral immunity. J Gen Virol 60: 225–233Google Scholar
- 8.Kilbourne ED, Horsfall FL Jr (1951) Studies of herpes simplex virus in newborn mice. J Immunol 67: 321–329Google Scholar
- 9.Lam MT, Pazin GJ, Armstrong JA, Ho M (1981) Herpes simplex virus infection in acute myelogenous leukemia and other hematologic malignancies: a prospective study. Cancer 48: 2168–2171Google Scholar
- 10.Levy RM, Bredesen DE, Rosenblum ML (1985) Neurological manifestations of the acquired immunodeficiency syndrome (AIDS): experience at UCSF and review of the literature. J Neurosurg 62: 475–495Google Scholar
- 11.Lopez C (1975) Genetics of natural resistance to herpesvirus infecitons in mice. Nature 258: 152–153Google Scholar
- 12.Mori R, Takeya K, Minamishima Y, Tasaki T (1965) Effect of thymectomy on experimental viral infections of mice. I Herpes simplex virus and Coxsackie B 5 virus. Proc Jap Acad 41: 975–978Google Scholar
- 13.Nagafuchi S, Oda H, Mori R, Taniguchi T (1979) Mechanism of acquired resistance to herpes simplex virus infection as studied in nude mice. J Gen Virol 44: 715–723Google Scholar
- 14.Nahmias AJ, Keyserling HL, Kerrick GM (1983) Herpes simplex. In: Remington JS, Klein JO (ed) Infectious diseases of the fetus and newborn infant, 2nd edn. WB Saunders, Philadelphia, pp 636–678Google Scholar
- 15.Naraqi S, Jackson GG, Jonasson O, Yamashiroya HM (1977) Prospective study of prevalence, incidence, and source of herpesvirus infections in patients with renal allografts. J Infect Dis 136: 531–540Google Scholar
- 16.Nash AA, Leung K-N, Wildy P (1985) The T-cell-mediated immune response of mice to herpes simplex virus. In: Roizman B, Lopez C (ed) The Herpesviruses, vol 4, immunobiology and prophylaxis of human herpesvirus infections. Plenum, New York London, pp 87–102Google Scholar
- 17.Oakes JE (1975) Role for cell-mediated immunity in the resistance of mice to subcutaneous herpes simplex virus infection. Infect Immun 12: 166–172Google Scholar
- 18.Otani T, Mori R (1987) The effects of ultraviolet irradiation of the skin on herpes simplex virus infection: alteration in immune function mediated by epidermal cells and in the course of infection. Arch Virol 96: 1–15Google Scholar
- 19.Potratz D, Brake B, Dienes HP, Schulz TF, Hosp M, Dierich MP, Falke D (1986) Herpes simplex virus type 1 and 2 in the adrenal glands: replication and histopathology. Arch Virol 90: 207–222Google Scholar
- 20.Rager-Zisman B, Allison AC (1976) Mechanism of immunologic resistance to herpes simplex virus 1 (HSV-1) infection. J Immunol 116: 35–40Google Scholar
- 21.Riepenhoff-Talty M, Dharakul T, Kowalski E, Michlak S, Ogra PL (1987) Persistent rotavirus infection in mice with severe combined immunodeficiency. J Virol 61: 3345–3348Google Scholar
- 22.Rouse BT (1984) Role of adaptive immune defense mechanisms in herpes simplex resistance. In: Rouse BT, Lopez C (ed) Immunobiology of herpes simplex virus infection. CRC Press, Boca Raton, FL, pp 131–144Google Scholar
- 23.Yamamoto M, Hayashi Y, Tang J-L, Mori R (1985) Effects of combined use of acyclovir and antibody in athymic nude mice inoculated intracutaneously with herpes simplex virus. Antiviral Res 5: 83–91Google Scholar
- 24.Yasumoto S, Okabe N, Mori R (1986) Role of epidermal Langerhans cells in resistance to herpes simplex virus infection. Arch Virol 90: 261–271Google Scholar
- 25.Yoshino K, Taniguchi S (1969) Isolation of a clone of herpes simplex virus highly attenuated for newborn mice and hamsters. Japan J Exp Med 39: 223–232Google Scholar