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Archives of Virology

, Volume 67, Issue 2, pp 187–189 | Cite as

Plaque assay ofHeliothis zea baculovirus employing a mixed agarose overlay

  • K. Yamada
  • K. Maramorosch
Brief Report

Summary

The nuclear polyhedrosis virus ofHeliothis zea has been titrated inHeliothis zea cells by the plaque method, using 1 percent mixed agarose containing a mixture of Seakem and Ultra pure agarose. Visible plaques, formed 8 days postinfection, ranged in diameter from 0.5 to 2 mm. Dose-response experiments indicated that a single particle initiated the formation of a plaque. The titration ofHeliothis zea baculovirus by the newly described plaque method provides an accurate technique for the determination of virus concentration.

Keywords

Agarose Infectious Disease Single Particle Plaque Assay Virus Concentration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • K. Yamada
    • 1
  • K. Maramorosch
    • 1
  1. 1.Waksman Institute of MicrobiologyRutgers UniversityPiscatawayUSA

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