Archives of Virology

, Volume 67, Issue 2, pp 187–189 | Cite as

Plaque assay ofHeliothis zea baculovirus employing a mixed agarose overlay

  • K. Yamada
  • K. Maramorosch
Brief Report


The nuclear polyhedrosis virus ofHeliothis zea has been titrated inHeliothis zea cells by the plaque method, using 1 percent mixed agarose containing a mixture of Seakem and Ultra pure agarose. Visible plaques, formed 8 days postinfection, ranged in diameter from 0.5 to 2 mm. Dose-response experiments indicated that a single particle initiated the formation of a plaque. The titration ofHeliothis zea baculovirus by the newly described plaque method provides an accurate technique for the determination of virus concentration.


Agarose Infectious Disease Single Particle Plaque Assay Virus Concentration 
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Copyright information

© Springer-Verlag 1981

Authors and Affiliations

  • K. Yamada
    • 1
  • K. Maramorosch
    • 1
  1. 1.Waksman Institute of MicrobiologyRutgers UniversityPiscatawayUSA

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