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Digestive Diseases and Sciences

, Volume 30, Issue 5, pp 445–455 | Cite as

Effects of intravenous infusion of amino acids, fat, or glucose on unstimulated pancreatic secretion in healthy humans

  • Claus Niederau
  • Amnon Sonnenberg
  • Joachim Erckenbrecht
Original Articles

Abstract

The effects of intravenous infusion of amino acids, fat, or glucose on unstimulated pancreatic secretion were studied in 31 healthy male volunteers. Each subject was studied twice on two separate days. On both days pancreatic outputs were measured during a 4-hr basal period that was followed by a 4-hr test period. During the test period either one of the digestive end products (100 ml/hr 10% amino acids in 13 subjects; 100 ml/hr 10% fat in 8 subjects; 150 ml/hr 10% glucose in 10 subjects) or saline was intravenously infused. The infusion of the digestive end products or saline on the two separate days was done in a randomized order. Six of the 10 subjects that had already been studied for glucose received a higher glucose dose (100 ml 20% glucose as a bolus followed by 300 ml/hr 10% glucose) in a third additional experiment. Intravenous infusion of amino acids significantly stimulated pancreatic outputs of trypsin and chymotrypsin, but left the outputs of amylase, lipase, bicarbonate, and volume unaffected. The low-glucose dose, as well as the fat infusion did not alter any of the pancreatic outputs. To analyze the relationships between different enzymes during the infusion of digestive end products, regression lines were calculated from the scatter of all individual pairs of enzyme measurements. Significantly different regression functions were found for each condition (NaCl control, amino acids, fat, glucose) when trypsin and chymotrypsin were plotted versus lipase and amylase: amino acids shifted the function to a trypsin- and chymotrypsin-dominant pattern of secretion, glucose to an amylase-dominant pattern, and fat to a lipasedominant pattern. These results demonstrate that although intravenous infusion of digestive end products had only minor effects on the overall amount of pancreatic secretion, circulating end products of digestion can alter the proportion of various digestive enzymes in a selective and short-term manner.

Keywords

Glucose Lipase Trypsin Amylase Intravenous Infusion 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    DiMagno EP, Go VLW, Summerskill WHJ: Intraluminal and postabsorptive effects of amino acids on pancreatic enzyme secretion. J Lab Clin Med 82:241–248, 1973Google Scholar
  2. 2.
    Ertan A, Brooks FP, Ostrow JD, Arbau DA, Williams CN, Cerda JC: Effects of jejunal amino acid perfusion and exogenous cholecystokinin on exocrine pancreatic and biliary secretion in man. Gastroenterology 61:686–692, 1971Google Scholar
  3. 3.
    Go VLW, Hofman AF, Summerskill WZ: Pancreozymin bioassay in man based on pancreatic enzyme secretion. Potency of specific amino acids and other digestive products. J Clin Invest 49:1558–1564, 1970Google Scholar
  4. 4.
    Froehlich C, Locher M, Oldershausen HF: Die Beeinflussung des exokrinen Pankreas durch die intravenoese Infusion einer Naehrloesung. Klin Wochenschr 51:1207–1209, 1973Google Scholar
  5. 5.
    Stastna R, Skala I, Hruba F, Shlygin GK, Sysoev Y: The effect of someN-solutions for parenteral nutrition on gastric and pancreatic secretion. Nutr Metab 23:349–356, 1979Google Scholar
  6. 6.
    Konturek SJ, Tasler JT, Cieszkowski M, Jaworek J, Konturek J: Intravenous amino acids and fat stimulate pancreatic secretion. Am J Physiol 236:E678-E684, 1979Google Scholar
  7. 7.
    Klein E, Shnebaum S, Ben-Ari H, Dreiling DA: Effects of total parenteral nutrition on exocrine pancreatic secretion. Am J Gastroenterol 78:31–33, 1983Google Scholar
  8. 8.
    Matsuno S, Miyashita E, Sasaki K, Sato T: Effects of intravenous fat emulsion administration on exocrine and endocrine pancreatic function. Jpn J Surg 11:323–329, 1981Google Scholar
  9. 9.
    Stabile BE, Borzatta M, Stubbs RS, Debas HT: Intravenous mixed amino acids and fats do not stimulate exocrine pancreatic secretion. Am J Physiol 246 (Gastrointest Liver Physiol 9):G274-G280, 1984Google Scholar
  10. 10.
    Fried GM, Ogden WD, Rhea A, Greeley G, Thompson JC: Pancreatic protein secretion and gastrointestinal hormone release in response to parenteral amino acids and lipid in dogs. Surgery 92:902–905, 1982Google Scholar
  11. 11.
    Edelmann K, Valenzuela JE: Effect of intravenous lipid on human pancreatic secretion. Gastroenterology 85:1063–1066, 1983Google Scholar
  12. 12.
    Grendell JH, Tseng HC, Rothman SS: Regulation of digestion. I. Effects of glucose and lysine on pancreatic secretion. Am J Physiol 246 (Gastrointest Liver Physiol 9):G445-G450, 1984Google Scholar
  13. 13.
    Malawer SJ, Powell DW: An improved turbidimetric analysis of polyethylen glycol utilizing an emulsifier. Gastroenterology 53:250–256, 1967Google Scholar
  14. 14.
    Erlanger BF, Kokowsky N, Cohen W: The preparation and the proper ties of two new chromogenic substrates of trypsin. Arch Biochem 95:217–278, 1961Google Scholar
  15. 15.
    Nagel W, Willig F, Peschke W, Schmidt FH: Ueber die Bestimmung von Trypsin und Chymotrypsin mit Amino-p-nitroaniliden. Z Physiol Chem 340:1–10, 1965Google Scholar
  16. 16.
    Haegele EO, Schaich E, Rauscher E, Lehmann P, Grassl M: Action pattern of human pancreatic alpha-amylase on maltoheptaose. J Chromatogr 223:69–84, 1981Google Scholar
  17. 17.
    Rick W: Diagnostische Verfahren, Klinisch-chemische Methoden.In Handbuch der inneren Medizin, Dritter Band, Teil 6, Pankreas. MM Forell (ed). Berlin, Springer 1976, pp 356–361Google Scholar
  18. 18.
    Lagerloef HO: Pancreatic function and disease. Acta Med Scand Suppl 128:1–13, 1942Google Scholar
  19. 19.
    Snedecor GW, Cochran WG: Statistical Methods, Chapter 10; Correlation. Ames, Iowa, The Iowa State University Press, 1980Google Scholar
  20. 20.
    Niederau C, Erckenbrecht J, Hellmann A, Sonnenberg A: Intravenoese Infusionen von Fettemulsionen mit mittel- und langkettigen Triglyzeriden (MCT/LCT) haben keine Auswirkungen auf die basale und die sekretinstimulierte Pankreassekretion beim Menschen. Z Gastroenterol 22:42–46, 1984Google Scholar
  21. 21.
    Zajtchuk R, Amato JC, Shoemaker WC, Baker RJ: The relationship between blood glucose levels and external pancreatic secretion in man. J Trauma 9:629–635, 1969Google Scholar
  22. 22.
    MacGregor IL, Deveney C, Way LW, Meyer JH: The effect of acute hyperglycemia on meal-stimulated gastric, biliary, and pancreatic secretion, and serum gastrin. Gastroenterology 70:197–202, 1976Google Scholar
  23. 23.
    Saito Y, Tokutake K, Matsuno S, Noto N, Honda T, Sato T: Effects of hypertonic glucose and amino acid infusions on pancreatic exocrine function. Tohoku J Exp Med 124:99–115, 1978Google Scholar
  24. 24.
    Raeder M, Mathisen Ø: Effect of intravenous infusion of hypertonic glucose solutions on pancreatic HCO3 secretion. Acta Physiol Scand 115:349–354, 1982Google Scholar
  25. 25.
    Sommer H, Kasper H: The action of synthetic secretin, cholecystokinin-octapeptide and combinations of these hormones on the secretion of the isolated perfused rat pancreas. Hepato-gastroenterol 28:311–315, 1981Google Scholar
  26. 26.
    Niederau C, Grendell JH, Rothman SS: The effects of proglumide on pancreatic secretion of digestive enzymes. Am J Physiol (submitted)Google Scholar
  27. 27.
    Rothman SS: Molecular regulation by the pancreas: Shortterm and bond-specific. Am J Physiol 226:77–83, 1974Google Scholar
  28. 28.
    Rothman SS: Independent secretion of different digestive enzymes by the pancreas. Am J Physiol 231:1847–1851, 1976Google Scholar
  29. 29.
    Grossman MI, Greengard H, Ivy AC: The effect of dietary composition on pancreatic enzymes. Am J Physiol 138:676–682, 1981Google Scholar
  30. 30.
    Marchis-Mouren G, Pasero L, Desnuelle P: Further studies of amylase biosynthesis by pancreas of rats fed on a starchrich or a casein-rich diet. Biochem Biophys Res Commun 13:262–266, 1963Google Scholar
  31. 31.
    Reboud JP, Marchis-Mouren G, Cozzone A, Desnuelle P: Variation in the biosynthesis rate of pancreatic amylase and chymotrypsinogen in response to a starch-rich or a proteinrich diet. Biochem Biophys Res Commun 22:94–99, 1966Google Scholar
  32. 32.
    Bucko A, Kopec Z: Adaptation of enzymes activity of the rat pancreas on altered food intake. Nutr Dieta Basel 10:276–287, 1968Google Scholar
  33. 33.
    Gidez LL: Effect of dietary fat on pancreatic lipase level in the rat. J Lipid Res 14:169–171, 1973Google Scholar
  34. 34.
    Snook JT: Dietary regulation of pancreatic enzymes in the rat with emphasis on carbohydrate. Am J Physiol 221:1383–1387, 1971Google Scholar
  35. 35.
    Walther AA: The secretory work of the pancreatic gland. PhD thesis. St. Petersberg, Russia, 1897Google Scholar
  36. 36.
    Pavlov IP: The Work of the Digestive Glands. London, Griffith, 1910.Google Scholar
  37. 37.
    Feigin RD, Beisel WR, Wannemacher RW: Rhythmicity of plasma amino acids and relation to dietary intake. Am J Nutr 24:329–336, 1971Google Scholar
  38. 38.
    Longenecker JB, Hause NL: Relationship between plasma amino acids and composition of the ingested food. Am J Nutr 9:356–362, 1961Google Scholar
  39. 39.
    Alfin-Slater RB, Aftergood L: Absorption, digestion, and metabolism of lipids.In Modern Nutrition in Health and Disease, 4th ed. MG Wohl, RS Goodhart (eds). Philadelphia, Lea and Febiger, 1968, pp 175–212Google Scholar
  40. 40.
    Levine R: Role of carbohydrates in the diet.In Modern Nutrition in Health and Disease, 4th ed. MG Wohl, RS Goodhart (eds). Philadelphia, Lea and Febiger, 1968, pp 156–174Google Scholar
  41. 41.
    Isenberg JI, Maxwell V: Intravenous infusion of amino acids stimulates gastric acid secretion in man. N Engl J Med 298:27–29, 1978Google Scholar
  42. 42.
    Konturek SJ, Kwiecien N, Obtulowicz W, Mikos E, Sito E, Olesky J: Comparison of intraduodenal and intravenous administration of amino acids on gastric secretion in healthy subjects and patients with duodenal ulcer. Gut 19:859–864, 1978Google Scholar
  43. 43.
    Dotevall G, Muren A: Effect of intravenous infusion of glucose on secretory responses to feeding in Pavlov- and Heidenhainpouch dogs. Acta Physiol Scand 52:234–241, 1961Google Scholar
  44. 44.
    Moore JG: Gastric acid suppression by intravenous glucose solutions. Gastroenterology 64:1106–1110, 1973Google Scholar
  45. 45.
    Johnson LR, Grossman MT: Effects of fat, secretin, and cholecystokinin on histamin-stimulated gastric secretion. Am J Physiol 216:1176–1179, 1969Google Scholar
  46. 46.
    Varner AA, Isenberg JI, Elashoff JD, Lamers CBHW, Maxwell V, Shulkes AA: Effect of intravenous lipid on gastric acid secretion stimulated by intravenous amino acids. Gastroenterology 79:873–876, 1980Google Scholar
  47. 47.
    Baume PE, Meng HG, Law DH: Intravenous fat emulsion and gastric acid secretion in the rat. Am J Dig Dis 11:1–9, 1966Google Scholar
  48. 48.
    Kotler DP, Levine GM: Reversible gastric and pancreatic hyposecretion after long-term total parenteral nutrition. N Engl J Med 300:241–242, 1979Google Scholar
  49. 49.
    Johnson LR, Schanbacher LM, Dudrick SJ, Copeland EM: Effect of long-term parenteral feeding on pancreatic secretion and serum secretin. Am J Physiol 233 (Endocrinology):E524-E529, 1977Google Scholar

Copyright information

© Plenum Publishing Corporation 1985

Authors and Affiliations

  • Claus Niederau
    • 1
  • Amnon Sonnenberg
    • 1
  • Joachim Erckenbrecht
    • 1
  1. 1.Department of Medicine DUniversity of DuesseldorfF.R. Germany

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