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Archives of Virology

, Volume 73, Issue 3–4, pp 287–294 | Cite as

Intrinsic interference between influenza A and B viruses

  • Angelica Mikheeva
  • Y. Z. Ghendon
Original Papers

Summary

Reproduction and synthesis of virus-specific macromolecules were studied in chick embryo fibroblast cultures co-infected with influenza viruses type A (FPV) and B (B/Japan/73). When a multiplicity of infection (MOI) of B/Japan/73 virus (10 EID50/cell and higher) was equal to, or exceeded that of FPV, formation of infectious FPV virions in coinfected cells was suppressed significantly. At equal MOI of FPV and B/Japan/73 synthesis of all proteins of one partner and some proteins of the other was observed. However, when a MOI of one virus was 10 times higher than that of the other, proteins of the virus used at a higher MOI were formed. Studies of the synthesis of virus-specific cRNAs formed in the presence of cycloheximide have shown that at equal MOI. cRNAs were detected that corresponded only to one of the partners involved in the reproduction. The data obtained suggest that intrinsic interference between A and B viruses occurs at a stage of primary transcription.

Keywords

Infectious Disease Influenza Macromolecule Influenza Virus Virus Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Bonloy, M., Plotch, S. J., Krug, R. M.: Globin mRNAs are primers for the transcription of influenza viral RNA in vitro. Proc. Nat. Acad. Sci. U.S.A.75, 4886–4890 (1978).Google Scholar
  2. 2.
    Bonner, W. M., Laskey, R. A.: A film detection method for tritium-labelled proteins and nucleic acids in polyacrylamide gels. Europ. J. Biochem.46, 83–88 (1970).Google Scholar
  3. 3.
    Davenport, F. M., Hennesy, A. V., Maasab, H. F., Minuse, E., Clark, L. C., Abrams, G. D., Mitchell, J. R.: Pilot studies on recombinant cold-adapted live type A and B influenza virus vaccines. J. Inf. Dis.136, 17–25 (1977).Google Scholar
  4. 4.
    Desselberger, U., Palese, P.: Molecular weights of RNA segments of influenza A and B viruses. Virology88, 394–399 (1978).Google Scholar
  5. 5.
    Desselberger, U., Racaniello, V. R., Zazra, J. J., Palese, P.: The 3' and 5'-terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene8, 315–328 (1980).Google Scholar
  6. 6.
    Ghendon, Y. Z., Klimov, A. I., Blagoveshenskaya, O. V., Genkina, D. B.: Investigation of recombinants of human influenza and fowl plague viruses. J. gen. Virol.43, 183–191 (1979).Google Scholar
  7. 7.
    Ghenkina, D. B., Ghendon, Y. Z.: Recombination and complementation between orthomyxoviruses under conditions of abortive infection. Acta Virol.23, 97–106 (1979).Google Scholar
  8. 8.
    Gottlieb, T., Hirst, G. K.: The experimental production of combined forms of virus. III. The formation of doubly antigenic particles from influenza A and B viruses and the study of the ability of individual particles of X viruses to yield two separate strains. J. Exp. Med.99, 304–320 (1954).Google Scholar
  9. 9.
    Hay, A. J., Lomniczi, B., Bellanei, A. R., Skehel, J. J.: Transcription of influenza virus genome. Virology83, 337–355 (1977).Google Scholar
  10. 10.
    Hugentobler, A. L., Schield, G. C., Oxford, J. S.: Differences in the electrophoretic migration rates of polypeptides and RNAs of recent isolates of influenza B viruses. Arch. Virol.69, 197–209 (1981).Google Scholar
  11. 11.
    Laemli, U. K.: Cleavage of structural proteins during the assembly of the heads of bacteriophage T4. Nature227, 680–685 (1970).Google Scholar
  12. 12.
    Lobmann, M., Delem, A., Jovanovic, D., Peetermans, J.: Recombinants of influenza virus type B as potential live vaccine candidates; RNA characterization and evaluation in man. J. Hyg. Camb.87, 43–52 (1981).Google Scholar
  13. 13.
    Moss, B., Keith, T. M., Gershowitz, A., Ritchey, M. B., Palese, P.: Common sequence of the 5' ends of the segmented RNA genomes of influenza A and B viruses. J. Virol.25, 312–318 (1978).Google Scholar
  14. 14.
    Plotch, S. J., Bonloy, M., Krug, R. M.: Transfer of 5'-terminal cap of globin mRNA to influenza viral complementary RNA during transcription in vitro. Proc. Nat. Acad. Sci. U.S.A.76, 1618–1622 (1979).Google Scholar
  15. 15.
    Racaniello, V. R., Palese, P.: Influenza B virus genome assignment of viral polypeptides to RNA segments. J. Virol.29, 361–373 (1979).Google Scholar
  16. 16.
    Rott, R., Orlich, M., Scholtissek, C.: Intrinsic interference between swine influenza and fowl plague virus. Arch. Virol.69, 25–39 (1981).Google Scholar
  17. 17.
    Russel, W. C., Skehel, J. J.: The polypeptide of adenovirus infected cells. J. gen. Virol.15, 45–57 (1972).Google Scholar
  18. 18.
    Scholtissek, C., Rohde, W., Harms, E.: Genetic relationship between an influenza A and B virus. J. gen. Virol.37, 243–247 (1977).Google Scholar
  19. 19.
    Skehel, J. J., Hay, A. J.: Nucleotide sequence at the 5' termini of influenza virus RNA and their transcripts. Nucleic Acid Res.5, 1207–1220 (1978).Google Scholar
  20. 20.
    Sugiura, A.: Influenza virus genetics. In:Kilbourne, E. D. (ed.), Influenza Virus and Influenza, 171–214. New York: Academic Press 1975.Google Scholar
  21. 21.
    Tobita, K., Ohori, K.: Heterotypic interference between influenza viruses A (Aichi) 2/68 and B (Massachusetts) 1/7. Acta Virol.23, 263–266 (1979).Google Scholar
  22. 22.
    Tumova, B., Kasova, V., Stumpa, A.: Mixed infections with influenza A and B viruses. Acta Virol.25, 418–419 (1981).Google Scholar

Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Angelica Mikheeva
    • 1
  • Y. Z. Ghendon
    • 1
  1. 1.Research Institute for Viral Preparations, MoscowMoscowUSSR

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