Archives of Virology

, Volume 50, Issue 1–2, pp 109–118 | Cite as

The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus

  • Jane K. A. Cook
  • J. H. Darbyshire
  • R. W. Peters
Original Papers


A study has been made of the use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis (AIB) virus from both naturally and experimentally infected chickens. Six strains of AIB virus were investigated, 3 of which had been isolated from natural outbreaks of disease. Two of the virus isolations from the outbreaks of AIB were made directly into tracheal organ cultures without passage in embryonated eggs.

Organ cultures prepared from 20-day-old embryos were used since they were found to be somewhat more sensitive in virus assay than those derived from chickens of up to 31 days of age. Ciliostasis, which was used as the marker of infectivity, was complete by 3 days after inoculation with each strain of virus examined.

Virus could be isolated from both respiratory and non-respiratory tissue in tracheal organ cultures and these cultures were found to be at least as sensitive as 9-day-old embryonated eggs in detecting AIB virus either in pathological material or in serial dilutions. When virus was assayed in both systems, the titres were very similar. It is considered, therefore, that chicken embryo tracheal organ cultures offer a reliable alternative system to embryonated eggs for studying AIB virus.


Infectious Disease Serial Dilution Organ Culture Bronchitis Virus Isolation 
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  1. 1.
    Anon: Methods for the examination of Poultry Biologics. National Academy of Sciences-National Research Council (1963).Google Scholar
  2. 2.
    Butler, M., Ellaway, Wendy J., Hall, T.: Comparative studies on the infectivity of avian respiratory viruses for eggs, cell cultures and tracheal explants. J. comp. Pathol.82, 327–332 (1972).Google Scholar
  3. 3.
    Cherry, J. D., Taylor-Robinson, D.: Large quantity production of chicken embryo tracheal organ cultures and use in virus and Mycoplasma studies. Appl. Microbiol.19, 658–662 (1970).Google Scholar
  4. 4.
    Colwell, W. M., Lukert, P. D.: Effects of avian infectious bronchitis virus (1 BV) on tracheal organ cultures. Avian Dis.13, 888–894 (1969).Google Scholar
  5. 5.
    Cunningham, C. H.: Avian infectious bronchitis. In:Brandly, C. A., Cornelius, C. E. (eds.), Adv. vet. Sci. comp. Med.14, 105–148. New York: Academic Press 1970.Google Scholar
  6. 6.
    Cunningham, C. H.: Immunity to avian infectious bronchitis. In:Perkins, F. T., Regamey, R. H., Hennessen, W. (eds.), International Symposium on Immunity to infections of the Respiratory System in Man and Animals 546–562. Basel: Karger 1975.Google Scholar
  7. 7.
    Darbyshire, J. H., Cook, Jane K. A., Peters, R. W.: Comparative growth kinetic studies on avian infectious bronchitis virus in different systems. J. comp. Pathol.85, 623–630 (1975).Google Scholar
  8. 8.
    Dulbecco, R., Vogt, M.: Plaque formation and isolation of pure lines with poliomyelitis viruses. J. exp. Med.99, 167–182 (1954).Google Scholar
  9. 9.
    Geilhausen, H. E., Ligon, F. B., Lukert, P. D.: The pathogenesis of virulent and avirulent avian infectious bronchitis virus. Arch. ges. Virusforsch.40, 285 to 290 (1973).Google Scholar
  10. 10.
    Harnett, G. B., Hooper, W. L.: Test-tube organ cultures of ciliated epithelium for the isolation of respiratory viruses. Lancet1, 339–340 (1968).Google Scholar
  11. 11.
    Hoorn, B., Tyrrell, D. A. J.: Organ cultures in Virology. Prog. med. Virol.11, 408–450 (1969).Google Scholar
  12. 12.
    Johnson, R. B., Newman, J. A., Wills, F. K.: Titration of infectious bronchitis virus in tracheas of susceptible and immune chickens. Avian Dis.13, 632–635 (1969).Google Scholar
  13. 13.
    Johnson, R. B., Newman, J. A.: Measurement of antigenic and pathogenic variability of infectious bronchitis virus strains by trachea culture. Avian Dis.15, 233–241 (1971).Google Scholar
  14. 14.
    Kawamura, H., Isogai, S., Tsubahara, H.: Propagation of avian infectious bronchitis virus in chicken kidney tissue culture. Nat. Inst. Anim. Hlth. Q., Tokyo1, 190–198 (1961).Google Scholar
  15. 15.
    McIntosh, K., Dees, Jane H., Becker, W. B., Kapikian, A. Z., Chanock, R. M.: Recovery in tracheal organ cultures of novel viruses from patients with respiratory disease. Proc. nat. Acad. Sci. U.S.A.57, 933–940 (1967).Google Scholar
  16. 16.
    Reed, L. J., Muench, H.: A simple method of estimating fifty percent end points. Amer. J. Hyg.27, 493–497 (1938).Google Scholar
  17. 17.
    Tyrrell, D. A. J., Bynoe, M. L.: Cultivation of viruses from a high proportion of patients with colds. Lancet1, 76–77 (1966).Google Scholar
  18. 18.
    Youngner, J. S.: Monolayer tissue cultures. 1. Preparation and standardization of suspensions of trypsin-dispersed monkey kidney cells. Proc. Soc. exp. Biol. Med.85, 202–205 (1954).Google Scholar

Copyright information

© Springer-Verlag 1976

Authors and Affiliations

  • Jane K. A. Cook
    • 1
  • J. H. Darbyshire
    • 1
  • R. W. Peters
    • 1
  1. 1.Microbiology DepartmentHoughton Poultry Research StationHoughton, HuntingdonEngland

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