Archives of Virology

, Volume 113, Issue 3–4, pp 291–296 | Cite as

Involvement of gangliosides in the interaction between BK virus and Vero cells

  • L. Sinibaldi
  • P. Goldoni
  • V. Pietropaolo
  • C. Longhi
  • N. Orsi
Brief Report

Summary

BK virus infectivity was inhibited by gangliosides extracted from Vero cells and by standard preparations of different gangliosides. Gangliosides were also able to restore the susceptibility of glycosidase-treated Vero cells to BK virus infection.

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References

  1. 1.
    Ando S, Chang NC, Yu RK (1978) High performance thin-layer chromatography and densitometric determination of brain ganglioside composition of several species. Anal Biochem 89: 437–450Google Scholar
  2. 2.
    Cahan LD, Paulson JC (1980) Polyoma virus adsorbs to specific sialyloligosaccharide receptors on erythrocytes. Virology 103: 505–509Google Scholar
  3. 3.
    Cahan LD, Singh R, Paulson JC (1983) Sialyloligosaccharide receptors of binding variants of polyomavirus. Virology 130: 281–289Google Scholar
  4. 4.
    De Stasio A, Mastromarino P, Pana' A, Seganti L, Sinibaldi L, Valenti P, Orisi N (1980) Characterization of a glycoprotein inhibitor present in human serum and active towards BK virus haemagglutination. Microbiologica 3: 293–305Google Scholar
  5. 5.
    Gardner SD, Field AM, Coleman DV, Hulme B (1971) New human papovavirus (BK) isolated from urine after renal transplantation. Lancet i: 1253–1257Google Scholar
  6. 6.
    Holmgren J, Svennerholm L, Elwing H, Fredman P, Stannegard O (1980) Sendai virus receptor: proposed recognition structure based on binding to plastic adsorbed gangliosides. Proc Natl Acad Sci USA 77: 1947–1950Google Scholar
  7. 7.
    Ledeen RW, Yu RK (1982) Gangliosides: structure, isolation and analysis. In: Ginsburg V (ed) Methods in enzymology, vol 83. Academic Press, New York, pp 139–191Google Scholar
  8. 8.
    Markwell MA, Svennerholm L, Paulson JC (1981) Specific gangliosides function as host cell receptors for Sendai virus. Proc Natl Acad Sci USA 78: 5406–5410Google Scholar
  9. 9.
    Markwell MA, Fredman P, Svennerholm L (1984) Receptor ganglioside content of three hosts for Sendai virus. Biochim Biophys Acta 775: 7–16Google Scholar
  10. 10.
    Mastromarino P, Conti C, Rieti S, Orsi N (1988) Identification of lipid components of human serum lipoproteins involved in the inhibition of Sindbis virus infectivity, hemagglutination and hemolysis. Arch Virol 103: 243–252Google Scholar
  11. 11.
    Roche AC, Maget-Dane R, Obrenovich A, Hildebrand K, Nicolau C, Monsigny M (1978) Interaction between vesicles containing gangliosides and limulin (Limulus poliphemus lectin). Fluorescence polarization of 1,6 diphenyl-1,3,5-hextriene. FEBS Lett 93: 91–96Google Scholar
  12. 12.
    Seganti L, Mastromarino P, Superti F, Paná A, Orsi N (1981) Comparative study of non-antibody inhibitors present in plasma of different animals and active towards BK virus, a human papovavirus. Microbiologica 4: 395–402Google Scholar
  13. 13.
    Seganti L, Mastromarino P, Superti F, Sinibaldi L, Orsi N (1986) Receptors for BK virus on human erythrocytes. Acta Virol 25: 177–181Google Scholar
  14. 14.
    Sinibaldi L, Goldoni P, Seganti L, Superti F, Tsiang H, Orsi N (1985) Gangliosides in early interactions between vesicular stomatitis virus and CER cells. Microbiologica 8: 355–365Google Scholar
  15. 15.
    Sinibaldi L, Viti D, Goldoni P, Cavallo G, Caroni C, Orsi N (1987) Inhibition of BK virus haemagglutination by gangliosides. J Gen Virol 68: 879–883Google Scholar
  16. 16.
    Superti F, Hauttecoeur B, Morelec MJ, Goldoni P, Bizzini B, Tsiang H (1986) Involvement of gangliosides in rabies virus infection. J Gen Virol 67: 47–56Google Scholar
  17. 17.
    Svennerholm L (1957) Quantitative estimation of sialic acids. II. A. Colorimetric resorcinol-hydrochloric acid method. Biochim Biophys Acta 24: 604–611Google Scholar
  18. 18.
    Svennerholm L, Fredman P (1980) A procedure for the quantitative isolation of brain gangliosides. Biochim Biophys Acta 617: 97–109Google Scholar
  19. 19.
    Tettamanti G, Bonali F, Marchesini S, Zambotti V (1973) A new procedure for the extraction, purification and fractionation of brain gangliosides. Biochim Biophys Acta 296: 160–170Google Scholar
  20. 20.
    Umeda M, Nojima S, Inoue K (1984) Activity of human erythrocyte gangliosides as a receptor for HVJ. Virology 133: 172–182Google Scholar
  21. 21.
    Williams MA, McCluer RH (1980) The use of Sep-Pak C18 Cartridges during the isolation of gangliosides. J Neurochem 35: 266–269Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • L. Sinibaldi
    • 1
  • P. Goldoni
    • 1
  • V. Pietropaolo
    • 1
  • C. Longhi
    • 1
  • N. Orsi
    • 1
  1. 1.Istituto di Microbiologia, Facoltà di Medicina e ChirurgiaUniversità di Roma “La Sapienza”Italy

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