Archives of Virology

, Volume 113, Issue 3–4, pp 267–277 | Cite as

Characterisation of an avian influenza virus nucleoprotein expressed inE. coli and in insect cells

  • V. R. Harley
  • K. A. Mather
  • B. E. Power
  • J. L. McKimm-Breschkin
  • P. J. Hudson
Brief Report


The nucleoprotein (NP) gene from influenza virus A/Shearwater/Australia/72 has been expressed intracellularly in bothE. coli and insect cells.E. coli-derived NP was identified by Western blot analysis as a 56 kDa protein which co-migrates with virion-derived NP. This protein was purified by immunoaffinity chromatography and a nitrocellulose binding assay showed that NP formed complexes with positive- and negative-sense influenza neuraminidase RNA transcribed in vitro. ELISA and Western blot analysis revealed that recombinant NP of 56 kDa was produced in high yields in insect cells using a baculovirus vector. Immunofluorescence microscopy revealed that NP was localised to the nucleus of infected insect cells.


Western Blot Infectious Disease Influenza Western Blot Analysis Influenza Virus 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • V. R. Harley
    • 1
  • K. A. Mather
    • 1
  • B. E. Power
    • 1
  • J. L. McKimm-Breschkin
    • 1
  • P. J. Hudson
    • 1
  1. 1.CSIRO Division of Biomolecular EngineeringParkvilleAustralia

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