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Archives of Virology

, Volume 113, Issue 3–4, pp 267–277 | Cite as

Characterisation of an avian influenza virus nucleoprotein expressed inE. coli and in insect cells

  • V. R. Harley
  • K. A. Mather
  • B. E. Power
  • J. L. McKimm-Breschkin
  • P. J. Hudson
Brief Report

Summary

The nucleoprotein (NP) gene from influenza virus A/Shearwater/Australia/72 has been expressed intracellularly in bothE. coli and insect cells.E. coli-derived NP was identified by Western blot analysis as a 56 kDa protein which co-migrates with virion-derived NP. This protein was purified by immunoaffinity chromatography and a nitrocellulose binding assay showed that NP formed complexes with positive- and negative-sense influenza neuraminidase RNA transcribed in vitro. ELISA and Western blot analysis revealed that recombinant NP of 56 kDa was produced in high yields in insect cells using a baculovirus vector. Immunofluorescence microscopy revealed that NP was localised to the nucleus of infected insect cells.

Keywords

Western Blot Infectious Disease Influenza Western Blot Analysis Influenza Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • V. R. Harley
    • 1
  • K. A. Mather
    • 1
  • B. E. Power
    • 1
  • J. L. McKimm-Breschkin
    • 1
  • P. J. Hudson
    • 1
  1. 1.CSIRO Division of Biomolecular EngineeringParkvilleAustralia

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