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Archives of Virology

, Volume 58, Issue 2, pp 127–136 | Cite as

Characterization of a Sindbis virus variant with altered host range

  • Janey Symington
  • M. J. Schlesinger
Article

Summary

A variant of Sindbis virus which is much more infectious for mouse cells than the standard virus has been examined for biochemical properties which might be responsible for this biological difference. The variant has a much enhanced ability to adsorb to mouse plasmacytoma (MOPC 315) cells, but when these cells were pretreated with heparin, they were able to adsorb the standard virus almost as well as the variant. This suggested that there was a surface charge difference between variant and standard virus. Differential elution of the viruses from hydroxyapatite and the results of isoelectric focusing of the virion glycoproteins substantiate this interpretation. Both viral glycoproteins E1 and E2 from the variant were more negatively charged than those of the standard virus but we were unable to find changes in tryptic peptides of the variant. Differences were found in stability of the two virus strains to heat and proteolytic enzymes.

Keywords

Peptide Heparin Hydroxyapatite Surface Charge Host Range 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Allison, A. C., Valentine, R. C.: Virus particle adsorption. III. Adsorption of viruses by cell monolayers and effects of some variables on adsorption. Biochim. biophys. Acta40, 400–410 (1960).Google Scholar
  2. 2.
    Basilico, C., di Majorca, G.: Mutant of polyomavirus with impaired adsorption to BHK Cells. J. Virol.13, 931–934 (1974).Google Scholar
  3. 3.
    Bengtsson, S., Dinter, Z., Philipson, L.: Genetic Markers associated with virulence of foot and mouth disease virus Proc. Soc. exp. Biol. Med.113, 1019–1022 (1963).Google Scholar
  4. 4.
    Bengtsson, S., Philipson, L., Pearson, H., Laurent, T. C.: The basis for the interaction between attenuated poliovirus and polyanions. Virology24, 617–625 (1964).Google Scholar
  5. 5.
    Bose, H. R., Brundige, M. A., Carl, G. Z., Sagik, B. P.: Peptide composition of Sindbis virus variants. Arch. ges. Virusforsch.31, 207–214 (1970).Google Scholar
  6. 6.
    Bose, H. R., Carl, G. Z., Sagik, B. P.: Separation of Sindbis plaque-type variants by calcium phosphate chromatography. Arch. ges. Virusforsch.29, 83–89 (1970).Google Scholar
  7. 7.
    Bose, H. R., Carl, G. Z., Sagik, B. P.: Purification of two plaque variants of Sindbis virus. Arch. ges. Virusforsch.31, 200–206 (1970).Google Scholar
  8. 8.
    Burge, B. W., Pfefferkorn, E. R.: Isolation and characteristics of temperature-sensitive mutants of Sindbis virus. Virology30, 204–213 (1966).Google Scholar
  9. 9.
    Cambell, J. B., Colter, J. S.: Isolation of a series of Mengo virus variants differing in sensitivity to polyanions. Canad. J. Microbiol.13, 651–657 (1967).Google Scholar
  10. 10.
    Clarke, D. H., Casals, J.: Techniques for hemagglutination and hemagglutination-inhibition with arthropod-borne viruses. Amer. J. trop. Med. Hyg.7, 561–573 (1958).Google Scholar
  11. 11.
    Dalrymple, J. M., Schlesinger, S., Russell, P. K.: Antigenic characterization of two Sindbis glycoproteins separated by isoelectric focusing. Virology69, 93–103 (1976).Google Scholar
  12. 12.
    Korant, B. D., Lonberg-Holm, K., Yin, F. H., Noble-Harvey, J.: Fractionation of biologically active and inactive populations of human rhinovirus Type 2. Virology63, 384–394 (1975).Google Scholar
  13. 13.
    Lomniczi, B.: Thermostability of Newcastle disease virus strains of different virulence. Arch. Virol.47, 249–255 (1975).Google Scholar
  14. 14.
    Mak, T. W., O'Callaghan, J., Colter, J. S.: Studies of the early events of Mengo encephalitis virus in mouse fibroblast cells. Virology42, 1087–1096 (1970).Google Scholar
  15. 15.
    Ozaki, Y., Kumagai, K.: Effect of polyions on Japanese encephalitis virus. Arch. ges. Virusforsch.39, 83–91 (1972).Google Scholar
  16. 16.
    Pederson, C. E., Jr., Barrera, C. R., Sagik, B. P.: Immunochemical studies of Sindbis virus variants. Arch. ges. Virusforsch.41, 28–39 (1973).Google Scholar
  17. 17.
    Schlesinger, S., Schlesinger, M. J.: Formation of Sindbis virus proteins: Identification of a precursor for one of the envelope proteins. J. Virol.10, 925–932 (1972).Google Scholar
  18. 18.
    Sefton, B. M., Keegstra, K.: Glycoproteins of Sindbis virus: Preliminary characterization of the oligosaccharides. J. Virol.14, 522–530 (1974).Google Scholar
  19. 19.
    Simpson, R. W., Hauser, R. E.: Effect of heat on virions of wild-type Sindbis virus and a thermostable mutant. Virology34, 361–364 (1968).Google Scholar
  20. 20.
    Symington, J., McCann, A. K., Schlesinger, M. J.: Infectious virus-antibody complexes of Sindbis virus. Infect. Immun.15, 720–725 (1977).Google Scholar
  21. 21.
    Symington, J., Schlesinger, M. J.: Isolation of a Sindbis virus variant by passage on mouse plasmacytoma cells. J. Virol.15, 1037–1041 (1975).Google Scholar
  22. 22.
    Takimoto, K. K., Liebhaber, H.: Virus polysaccharide interactions. II. Enhancement of plaque formation and the detection of variants of poliovirus with dextran sulfate. Virology17, 499–501 (1962).Google Scholar
  23. 23.
    Vaheri, A.: Heparin and related polyanionic substances as virus inhibitors. Acta Pathol. Microbiol. Scand. Suppl.171, 1–98 (1964).Google Scholar
  24. 24.
    Walder, R., Liprandi, F.: Kinetics of heat inactivation of Venezuelan equine encephalomyelitis virus. Arch. Virol.51, 307–317 (1976).Google Scholar
  25. 25.
    Wittmann, G., Dietzschold, B., Bauer, K.: Some investigations on the adjuvant mechanism of DEAE-Dextran. Arch. Virol.47, 225–235 (1975).Google Scholar

Copyright information

© Springer-Verlag 1978

Authors and Affiliations

  • Janey Symington
    • 1
  • M. J. Schlesinger
    • 1
  1. 1.Department of Microbiology and ImmunologyWashington University Medical School, Division of Biology and Biomedical SciencesSt. LouisUSA

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