Archives of Virology

, Volume 140, Issue 5, pp 937–944

MDCK cell cultures supplemented with high concentrations of trypsin exhibit remarkable susceptibility to influenza C virus

  • M. Yamaoka
  • M. Homma
  • H. Hotta
Brief Report

DOI: 10.1007/BF01314969

Cite this article as:
Yamaoka, M., Homma, M. & Hotta, H. Archives of Virology (1995) 140: 937. doi:10.1007/BF01314969


Multiplication of influenza C virus in MDCK cell cultures increased with increasing concentrations of trypsin up to 160 µg/ml, whereas maximum growth of influenza A virus in the same culture was observed at a concentration of 10 µg/ml. In the presence of 160 µg of trypsin per ml MDCK cells showed the same or even higher susceptibility to various strains of influenza C virus compared with HMV-II cells, a human melanoma cell line that has been reported to have high susceptibility to the virus. Complete cleavage of the HE precursor protein of MDCK-grown influenza C virus into HE1 and HE2 subunits was achieved by trypsin at a concentration of 160 µg/ml, whereas only partial cleavage was observed at 10 µg/ml. The present results thus demonstrate that MDCK cell cultures supplemented with trypsin at a concentration of 160 µg/ml become highly susceptible to influenza C virus.

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • M. Yamaoka
    • 1
  • M. Homma
    • 2
  • H. Hotta
    • 3
  1. 1.Division of MicrobiologyThe Hyogo Prefectural Institute of Public HealthKobe, HyogoJapan
  2. 2.Faculty of Home EconomicsKobe Women's UniversityKobe, HyogoJapan
  3. 3.Department of MicrobiologyKobe University School of MedicineKobe, HyogoJapan

Personalised recommendations