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Archives of Virology

, Volume 71, Issue 2, pp 177–183 | Cite as

A modified plaque assay method for accurate analysis of infectivity of influenza viruses with uncleaved hemagglutinin

  • O. P. Zhirnov
  • A. V. Ovcharenko
  • A. G. Bukrinskaya
Original Papers

Summary

A modified plaque assay method for evaluation of infectivity of influenza viruses is described. The modification consists of a double agar overlay, trypsin being included in the second layer added 24 hours after infection. The modified method allows one to determine accuratelly the infectivity titer of influenza viruses possessing uncleaved hemagglutinin, and to compare the infectivity of viruses possessing cleaved or uncleaved forms of hemagglutinin. The standard method in which trypsin is included into agar overlay just after infection artifically increases the infectivity titer of viruses with uncleaved hemagglutinin and therefore is not suitable for these purposes.

Keywords

Agar Infectious Disease Influenza Trypsin Standard Method 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • O. P. Zhirnov
    • 1
  • A. V. Ovcharenko
    • 1
  • A. G. Bukrinskaya
    • 1
  1. 1.The D. I. Ivanovsky Institute of VirologyThe U.S.S.R. Academy of Medical SciencesMoscowU.S.S.R.

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