Archives of Virology

, Volume 54, Issue 4, pp 307–315

The preferential cytotoxicity of reovirus for certain transformed cell lines

  • G. Hashiro
  • P. C. Loh
  • Jenny T. Yau
Original Papers

Summary

The susceptibility of a variety of cell lines of different mammalian origin to cytotoxic (CT) induction by either ultraviolet light-irradiated reovirus type 2 (UVR2) or viable reovirus type 2 plus the protein synthesis inhibitor, cycloheximide, was examined. The following groups of cells were found to be susceptible to CT-induction: certain tumor cells and spontaneously transformed cell lines of human origin and certain virally and spontaneously transformed cell lines of murine origin. The following groups of cells were found to be resistant: normal human diploid cell lines, primary and continuous cell cultures of subhuman primates, primary mouse cells, normal rat kidney cells and baby hamster kidney cells. Susceptibility to CT-induction could not be related to the adsorption of virus to cells, nor to the capacity of the cell to support virus replication.

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References

  1. 1.
    Borsa, J., Graham, A. F.: Reovirus: RNA polymerase activity in purified virions. Biochem. biophys. Res. Commun.33, 895–901 (1968).Google Scholar
  2. 2.
    Hand, R., Tamm, I.: Reovirus: Effect of noninfective viral components on cellular deoxyribonucleic acid synthesis. J. Virol.11, 223–231 (1973).Google Scholar
  3. 3.
    Lai, M. T., Joklik, W.: The induction of interferon by temperature-sensitive mutants of reovirus, UV-irradiated reovirus, and subviral reovirus particles. Virology51, 191–204 (1973).Google Scholar
  4. 4.
    Lai, M. T., Wérenne, J. J., Joklik, W.: The preparation of reovirus top component and its effect on host DNA and protein synthesis. Virology54, 237–244 (1973).Google Scholar
  5. 5.
    Loh, P. C., Crowley, J.: Reovirus type 2 infection, cycloheximide and cell death. Proc. Soc. exp. Biol. Med.125, 1287–1290 (1969).Google Scholar
  6. 6.
    Loh, P. C., Oie, H. K.: Growth characteristics of reovirus type 2: Ultraviolet light inactivated virion preparations and cell death. Arch. ges. Virusforsch.26, 197–208 (1969).Google Scholar
  7. 7.
    Loh, P. C., Oie, H. K., Camyre, K. P.: Role of lysine in the replication of reovirus II. Characterization of empties and effect on macromolecular synthesis. Arch. ges. Virusforsch.35, 114–125 (1971).Google Scholar
  8. 8.
    Loh, P. C., Oie, H. K., Ratnayake, R. M.: Accelerated cytopathology in HeLa cells induced by reovirus and cycloheximide. Infect. Immun.2, 705–712 (1970).Google Scholar
  9. 9.
    Loh, P. C., Shatkin, A. J.: Structural proteins of reovirus. J. Virol.2, 1353–1359 (1968).Google Scholar
  10. 10.
    Shatkin, A. J., Sipe, J. D.: RNA polymerase activity in purified reoviruses. Proc. Natl. Acad. Sci. U.S.A.61, 1462–1469 (1968).Google Scholar
  11. 11.
    Shaw, J. E., Cox, D. C.: Early inhibition of cellular DNA synthesis by high multiplicities of infections and UV-inactivated reovirus. J. Virol.12, 704–710 (1973).Google Scholar
  12. 12.
    Subashinghe, H. A., Loh, P. C.: Reovirus cytotoxicity: some properties of the UV-irradiated reovirus and its capsid proteins. Arch. ges. Virusforsch.39, 172 to 189 (1972).Google Scholar
  13. 13.
    Watanabe, Y., Kudo, H., Graham, A. F.: Selective inhibition of reovirus ribonucleic acid synthesis by cycloheximide. J. Virol.1, 36–44 (1967).Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • G. Hashiro
    • 1
  • P. C. Loh
    • 1
  • Jenny T. Yau
    • 1
  1. 1.Virus Laboratory, Department of MicrobiologyUniversity of HawaiiHonoluluUSA

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