Archives of Virology

, Volume 66, Issue 3, pp 215–226 | Cite as

Astrovirus-like, coronavirus-like, and parvovirus-like particles detected in the diarrheal stools of beagle pups

  • F. P. WilliamsJr.
Original Papers


Astrovirus-like, coronavirus-like, and parvovirus-like particles were detected through electron microscopic (EM) examination of loose and diarrheal stools from a litter of beagle pups. Banding patterns obtained from equilibrium centrifugations in CsCl supported the EM identification. Densities associated with the identified particles were: 1.34 g/ml for astrovirus, 1.39 g/ml for “full” parvovirus, and 1.24–1.26 g/ml for “typical” coronavirus. Convalescent sera from the pups aggregated these three particle types as observed by immunoelectron microscopy (IEM). Only coronavirus-like particles were later detected in formed stools from these same pups. Coronavirus and parvo-like viruses are recognized agents of canine viral enteritis, however, astrovirus has not been previously reported in dogs.


Electron Microscopic Infectious Disease Banding Pattern CsCl Particle Type 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Appel, M. J. G., Cooper, B. J., Greisen, H., Carmichael, L. E.: Status report: canine viral enteritis. J. Amer. vet. med. Ass.173, 1516–1518 (1978).Google Scholar
  2. 2.
    Ashley, C. R., Caul, E. O., Paver, W. K.: Astrovirus-associated gastroenteritis in children. J. clin. Pathol.31, 939–943 (1978).Google Scholar
  3. 3.
    Bachmann, P. A., Hoggan, M. D., Kurstak, E., Melnick, J. L., Pereira, H. G., Tattersall, P., Vago, C.: Parvoviridae: Second report. Intervirology11, 248 to 254 (1979).Google Scholar
  4. 4.
    Binn, L. N., Lazar, E. C., Eddy, G. A., Kajima, M.: Recovery and characterization of a minute virus of canines. Infect. Immun.1, 503–508 (1970).Google Scholar
  5. 5.
    Binn, L. N., Lazar, E. C., Keenan, K. P., Huxsoll, D. L., Marchwicki, R. H., Strano, A. J.: Recovery and characterization of a coronavirus from military dogs with diarrhea. In: Proc. 78th Meeting, U.S. Animal Health Assoc., Roanoke, Va., October 1974, 359–366. Richmond: U.S. Animal Health Assoc. 1975.Google Scholar
  6. 6.
    Burtonboy, G., Coignoul, F., Delferriere, N., Pastoret, P. P.: Canine hemorrhagic enteritis: detection of viral particles by electron microscopy. Arch. Virol.61, 1–11 (1979).Google Scholar
  7. 7.
    Caul, E. O., Ashley, C. R., Egglestone, S. I.: Recognition of human enteric coronaviruses by electron microscopy. Med. Lab. Sci.34, 259–263 (1977).Google Scholar
  8. 8.
    Caul, E. O., Egglestone, S. I.: Further studies on human enteric coronaviruses. Arch. Virol.54, 107–117 (1977).Google Scholar
  9. 9.
    Cooper, B. J., Carmichael, L. E., Appel, M. J. G., Greisen, H.: Canine viral enteritis. II. Morphologic lesions in naturally occurring parvovirus infection. Cornell Vet.69, 134–144 (1979).Google Scholar
  10. 10.
    Eugster, A. K., Bendele, R. A., Jones, L. P.: Parvovirus infection in dogs. J. Amer. vet. med. Ass.173, 1340–1341 (1978).Google Scholar
  11. 11.
    Flewett, T. H., Bryden, A. S., Davies, H. A., Woode, G. N., Bridger, J. C., Derrick, J. M.: Relation between viruses from acute gastroenteritis of children and newborn calves. Lancetii,61–63 (1974).Google Scholar
  12. 12.
    Flewett, T. H., Bryden, A. S., Davies, H. A.: Diagnostic electron microscopy of faeces. I. The viral flora of the faeces as seen by electron microscopy. J. clin. Pathol.27, 603–608 (1974).Google Scholar
  13. 13.
    Hajer, I., Storz, J.: Structural polypeptides of the enteropathogenic bovine coronavirus strain LY-138. Arch. Virol.59, 47–57 (1979).Google Scholar
  14. 14.
    Keenan, K. P., Jevis, H. R., Marchwicki, R. H., Binn, L. N.: Intestinal infection of neonatal dogs with canine coronavirus 1–71: studies by virologic, histologic, histochemical and immunofluorescent techniques. Amer. J. vet. Res.37, 247 to 256 (1976).Google Scholar
  15. 15.
    Kurtz, J. B., Lee, T. W., Pickering, D.: Astrovirus associated gastroenteritis in a children's ward. J. clin. Pathol.30, 948–952 (1977).Google Scholar
  16. 16.
    Maass, G., Baumeister, H. G., Hergemoller, R., Jansen, P., Schumacher, H.: Visualization by electronmicroscopy of 28 nm virusparticles (astroviruses) in faeces of newborns with acute nonbacterial gastroenteritis. Zbl. Bakt. Hyg., I. Abt. Orig.A242, 423–430 (1978).Google Scholar
  17. 17.
    McIntosh, K.: Coronaviruses: a comparative review. Curr. Top. Microbiol. Immunol.63, 85–129 (1974).Google Scholar
  18. 18.
    Madeley, C. R., Cosgrove, B. P.: Viruses in infantile gastroenteritis. Lancetii, 124 (1975).Google Scholar
  19. 19.
    Madeley, C. R., Cosgrove, B. P.: 28 nm particles in faeces in infantile gastroenteritis. Lancetii, 451–452 (1975).Google Scholar
  20. 20.
    Madeley, C. R., Cosgrove, B. P.: Calicivirus in man. Lanceti, 199–200 (1976).Google Scholar
  21. 21.
    Madeley, C. R.: Comparison of the features of astroviruses and caliciviruses seen in samples of feces by electron microscopy. J. inf. Dis.139, 519–523 (1979).Google Scholar
  22. 22.
    Mathan, M., Mathan, V. I., Swaminathan, S. P., Yesudoss, S., Baker, S. J.: Pleomorphic virus-like particles in human faeces. Lanceti, 1068–1069 (1975).Google Scholar
  23. 23.
    Middleton, P. J., Szymanski, M. T., Petric, M.: Viruses associated with acute gastroenteritis in young children. Amer. J. Dis. Child.131, 733–737 (1977).Google Scholar
  24. 24.
    Pocock, D. H.: Effect of sulphydryl reagents on the biological activities, polypeptide composition and morphology of haemagglutinating encephalomyelitis virus. J. gen. Virol.40, 93–101 (1978).Google Scholar
  25. 25.
    Pollock, R. V. H., Carmichael, L. E.: Canine viral enteritis. Mod. Vet. Pract.60, 375–380 (1979).Google Scholar
  26. 26.
    Siegl, G.: The Parvoviruses (Virology Monographs, Vol. 15), 109. Wien-New York: Springer 1976.Google Scholar
  27. 27.
    Snodgrass, D. R., Gray, E. W.: Detection and transmission of 30 nm virus particles (astroviruses) in faeces of lambs with diarrhoea. Arch. Virol.55, 287–291 (1977).Google Scholar
  28. 28.
    Snodgrass, D. R., Angus, K. W., Gray, E. W., Menzies, J. D., Paul, G.: Pathogenesis of diarrhea caused by astrovirus infections in lambs. Arch. Virol.60, 217–226 (1979).Google Scholar
  29. 29.
    Spratt, H. C., Marks, M. I., Gomersall, M., Gill, P., Pai, C. H.: Nosocomial infantile gastroenteritis associated with minirotavirus and calicivirus. J. Pediatr.93, 922–926 (1978).Google Scholar
  30. 30.
    Stair, E. L., Rhodes, M. B., White, R. G., Mebus, C. A.: Neonatal calf diarrhea: purification and electron microscopy of a coronavirus-like agent. Amer. J. vet. Res.33, 1147–1156 (1972).Google Scholar
  31. 31.
    Tyrrell, D. A. J., Alexander, D. J., Almeida, J. D., Cunningham, C. H., Easterday, B. C., Garwes, D. J., Hierholzer, J. C., Kapikian, A., Macnaughton, M. R., McIntosh, K.: Coronaviridae: Second report. Intervirology10, 321 to 328 (1978).Google Scholar
  32. 32.
    Woode, G. N., Bridger, J. C.: Isolation of small viruses resembling astroviruses and calciviruses from acute enteritis of calves. J. med. Microbiol.11, 441–452 (1978).Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • F. P. WilliamsJr.
    • 1
  1. 1.Health Effects Research LaboratoryU.S. Environmental Protection AgencyCincinnatiUSA

Personalised recommendations