Archives of Virology

, Volume 66, Issue 3, pp 193–206 | Cite as

Structural polypeptides of canine distemper virus

  • C. Örvell
Original Papers
Received:
Accepted:

Summary

The structural polypeptides of two strains of canine distemper virus and the Lec strain of measles virus were analysed by SDS-polyacrylamide-slab-gel electrophoresis. One strain of canine distemper virus derived from a live vaccine (Convac, Dumex), contained six major structural polypeptides with mol. wt. of 85, 78, 59, 43, 41 and 34×103. The 85K polypeptide was glycosylated. It was interpreted to be equivalent to the 79K glycoprotein of the measles hemagglutinin.

The second strain, a rapidly growing variant of the Onderstepoort strain of canine distemper virus characterized by extensive syncytium forming cytopathic effects in tissue culture, contained the 59, 43, 41 and 34K polypeptides, but the 85 and 78K polypeptides were not present in detectable amounts. The 43K polypeptide was identified as cellular actin by limited proteolysis. By use of monospecific rabbit hyperimmune sera against each of the major structural polypeptides of measles virus, the 59, 41 and 34K structural polypeptides could be identified as nucleocapsid protein (NP), fusion (F) polypeptide, and the membrane (M) polypeptide, respectively. In neutralization tests with rabbit hyperimmune sera against each of the two strains, this Onderstepoort strain, which contained reduced amounts of the hemagglutinin glycoprotein, gave higher neutralization titers than the vaccine strain.

Keywords

Polypeptide Measle Vaccine Strain Measle Virus Neutralization Test 
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References

  1. 1.
    Anderson, C. W., Baum, P. R., Gesteland, R. F.: Processing of adenovirus 2-induced proteins. J. Virol.12, 241–252 (1973).Google Scholar
  2. 2.
    Barbanti-Brodano, G., Oyanagi, S., Katz, M., Koprowski, H.: Presence of two different viral agents in brain cells of a patient with subacute sclerosing panencephalitis. Proc. Soc. exp. Biol. Med.134, 230–236 (1970).Google Scholar
  3. 3.
    Bussell, R. H., Waters, D. J., Seals, M. K. J., Robinson, W. S.: Measles, canine distemper and respiratory syncytial virions and nucleocapsids. A comparative study of their structure, polypeptide and nucleic acid composition. Med. Microbiol. Immunol.160, 105–124 (1974).Google Scholar
  4. 4.
    Chiarini, A., Norrby, E.: Separation and characterization of products of two measles virus variants. Arch. Virol.29, 205–214 (1970).Google Scholar
  5. 5.
    Cleveland, D. W., Fischer, S. G., Kirschner, M. W., Laemmli, U. K.: Peptide mapping by limited proteolysis in sodium dodecyl sulfate and analysis by gelelectrophoresis. J. biol. Chem.252, 1102–1106 (1977).Google Scholar
  6. 6.
    Graves, M. C., Silver, S. M., Choppin, P. W.: Measles virus polypeptide synthesis in infected cells. Virology86, 254–263 (1978).Google Scholar
  7. 7.
    Hall, W. W., Lamb, R. A., Choppin, P. W.: Measles and subacute sclerosing panencephalitis virus proteins. Lack of antibodies to the M protein in patients with subacute sclerosing panencephalitis. Proc. Nat. Acad. Sci. U.S.A.76, 2047 to 2051 (1979).Google Scholar
  8. 8.
    Hall, W. W., Lamb, R. A., Choppin, P. W.: The polypeptides of canine distemper virus: Synthesis in infected cells and relatedness to the polypeptides of other morbilliviruses. Virology100, 433–449 (1980).Google Scholar
  9. 9.
    Hardwick, J. M., Bussell, R. H.: Glycoproteins of measles virus under reducing and nonreducing conditions. J. Virol.25, 687–692 (1978).Google Scholar
  10. 10.
    Kingsbury, D. W., Bratt, M. A., Choppin, P. W., Hanson, R. P., Hosaka, Y., ter Meulen, V., Norrby, E., Plowright, W., Rott, R., Wunner, W. H.: Paramyxoviridae. Intervirology10, 137–152 (1978).Google Scholar
  11. 11.
    Laemmli, U. K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature227, 680–685 (1970).Google Scholar
  12. 12.
    Lamb, R. A., Mahy, B. W. J., Choppin, P. W.: The synthesis of Sendai virus polypeptides in infected cells. Virology69, 116–131 (1976).Google Scholar
  13. 13.
    Lamb, R. A., Choppin, P. W.: The synthesis of Sendai virus polypeptides in infected cells. III. Phosphorylation of polypeptides. Virology81, 382–397 (1977).Google Scholar
  14. 14.
    Lamb, R. A., Choppin, P. W.: Determination by peptide mapping of the unique polypeptides in Sendai virions and infected cells. Virology84, 469–478 (1978).Google Scholar
  15. 15.
    Martin, S. J., ter Meulen, V.: A rapid method for the quantitative study of RNA from canine distemper virus infected cells. J. gen. Virol.32, 321–325 (1976).Google Scholar
  16. 16.
    Merz, D. C., Scheid, A., Choppin, P. W.: The importance of antibodies to the fusion glycoprotein (F) of paramyxoviruses in the prevention of spread of infection. J. exp. Med.151, 275–288 (1980).Google Scholar
  17. 17.
    Mountcastle, W. E., Choppin, P. W.: A comparison of the polypeptides of four measles virus strains. Virology78, 463–474 (1977).Google Scholar
  18. 18.
    Norrby, E., Gollmar, Y.: Appearance and persistence of antibodies against different virus components after regular measles infections. Infect. Immun.6, 240–247 (1972).Google Scholar
  19. 19.
    Norrby, E., Gollmar, Y.: Identification of measles virus-specific hemolysis-inhibiting antibodies separate from hemagglutination-inhibiting antibodies. Infect. Immun.11, 231–239 (1975).Google Scholar
  20. 20.
    Norrby, E., Grandien, M., Örvell, C.: New tests for characterization of mumps virus antibodies: hemolysis-inhibition, single radial immunodiffusion with immobilized virions, and mixed hemadsorption. J. clin. Microbiol.5, 346–352 (1977).Google Scholar
  21. 21.
    Örvell, C., Norrby, E.: Further studies on the immunologic relationships among measles, distemper and rinderpest viruses. J. Immunol.113, 1850–1858 (1974).Google Scholar
  22. 22.
    Örvell, C.: Immunological properties of purified mumps virus glycoproteins. J. gen. Virol.41, 517–526 (1978).Google Scholar
  23. 23.
    Örvell, C.: Structural polypeptides of mumps virus. J. gen. Virol.41, 527–539 (1978).Google Scholar
  24. 24.
    Rima, B. K., Martin, S. J., Gould, E. A.: A comparison of polypeptides in measles and SSPE virus strains. J. gen. Virol.42, 603–608 (1979).Google Scholar
  25. 25.
    Rockborn, G.: Canine distemper virus in tissue culture. Arch. Virol.8, 485–492 (1958).Google Scholar
  26. 26.
    Scheid, A., Choppin, P. W.: Two disulfide-linked polypeptide chains constitute the active F protein of paramyxoviruses. Virology80, 54–66 (1977).Google Scholar
  27. 27.
    Spudich, J. A., Watt, S.: The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin. J. biol. Chem.246, 4866–4871 (1971).Google Scholar
  28. 28.
    Stallcup, K. C., Wechsler, S. L., Fields, B. N.: Purification of measles virus and characterization of subviral components. J. Virol.30, 166–176 (1979).Google Scholar
  29. 29.
    Tyrrell, D. L. J., Norrby, E.: Structural polypeptides of measles virus. J. gen. Virol.39, 219–229 (1978).Google Scholar
  30. 30.
    Tyrrell, D. L. J., Ehrnst, A.: Transmembrane communication in cells chronically infected with measles virus. J. cell Biol.81, 396–402 (1979).Google Scholar
  31. 31.
    Vainionpää, R., Ziola, B., Salmi, A.: Measles virus polypeptides in purified virions and in infected cells. Acta Path. Microbiol. Scand.B86, 379–385 (1978).Google Scholar
  32. 32.
    Waters, D. J., Bussell, R. H.: Polypeptide composition of measles and canine distemper virus. Virology55, 554–557 (1973).Google Scholar
  33. 33.
    Wechsler, S. L., Fields, B. N.: Intracellular synthesis of measles virus-specified polypeptides. J. Virol.25, 285–297 (1978).Google Scholar
  34. 34.
    Wechsler, S. L., Fields, B. N.: Differences between the intracellular polypeptides of measles and subacute sclerosing panencephalitis virus. Nature272, 458–460 (1978).Google Scholar
  35. 35.
    Wechsler, S. L., Weiner, H. L., Fields, B. N.: Immune response in subacute sclerosing panencephalitis: reduced antibody response to the matrix protein of measles virus. J. Immunol.123, 884–889 (1979).Google Scholar

Copyright information

© Springer-Verlag 1980

Authors and Affiliations

  • C. Örvell
    • 1
  1. 1.Department of VirologyKarolinska Institutet, School of MedicineStockholmSweden

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