Archives of Virology

, Volume 74, Issue 2–3, pp 233–238 | Cite as

The production of varicella zoster virus antiserum in laboratory animals

  • Zofia Wroblewska
  • M. Devlin
  • K. Reilly
  • H. van Trieste
  • M. Wellish
  • D. H. Gilden
Brief Report

Summary

Hyperimmune anti-varicella zoster virus (VZV) antisera were prepared in BALB/c mice, Lewis rats and New Zealand rabbits by subcutaneous inoculations of purified VZ virions suspended in Freund's adjuvant. VZV antibodies were demonstrated both by an enzyme-linked immunosorbent assay (ELISA) and by indirect immunofluorescence (IF). VZV could not be detected in organs of any inoculated animals by IF or by cocultivation of tissue fragments from infected animals with human diploid fibroblasts.

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Copyright information

© Springer-Verlag 1982

Authors and Affiliations

  • Zofia Wroblewska
    • 1
  • M. Devlin
    • 2
  • K. Reilly
    • 1
  • H. van Trieste
    • 1
  • M. Wellish
    • 2
  • D. H. Gilden
    • 1
    • 2
  1. 1.The Multiple Sclerosis Research Center of the Wistar InstitutePhiladelphiaUSA
  2. 2.the Department of Neurology of the University of Pennsylvania School of MedicinePhiladelphiaUSA

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