Archives of Virology

, Volume 101, Issue 1–2, pp 137–140 | Cite as

An improved procedure for the rapid one-step-cloning of full-length viroid cDNA

  • H. Puchta
  • H. L. Sänger
Rapid Communication


The efficiency of viroid cloning can be increased by three to four orders of magnitude when the synthesis of viroid cDNA is primed in such a way that it carries identical sticky ends on both termini and when the multiion transformation is applied.


Infectious Disease Improve Procedure Viroid cDNA 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • H. Puchta
    • 1
  • H. L. Sänger
    • 1
  1. 1.Abteilung ViroidforschungMax-Planck-Institut für BiochemieMartinsried bei MünchenFederal Republic of Germany

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