Enzyme-linked immunosorbent assay for detection of arenaviruses
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Conditions for ELISA by using IgG linked with horseradish peroxidase have been developed for the detection of 6 arenaviruses (Machupo, Junin, Tacaribe, Amapari, LCM and Tamiami). This method allows the detection of arenavirus antigens in various materials; organs and blood of infected animals and infected cell culture fluids. It shows higher sensitivity and allows an earlier detection of virus-specific antigens as compared with the CF test.
KeywordsCell Culture Infectious Disease Early Detection Immunosorbent Assay Infected Cell
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- 1.Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. biol. Chem.193, 265–275 (1951).Google Scholar
- 2.Mathiesen, L. S., Feinstone, S. M., Wong, D. C., Skinhoe, J. P., Purcell, R. H.: Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy and immune adherence hemagglutination assay. J. clin. Microbiol.7, 184–193 (1978).Google Scholar
- 3.Nakane, P. K., Kawaoi, A.: Peroxidase-labelled antibody: a new method of conjugation. J. Histochem. Cytochem.22, 1084–1091 (1974).Google Scholar
- 4.Voller, A., Bidwell, D. E., Barlett, A.: Enzyme immunoassays in diagnostic medicine. Bull. World Health Organ.53, 55–66 (1976).Google Scholar