Archives of Virology

, Volume 55, Issue 1–2, pp 77–86 | Cite as

Detection of transmissible gastroenteritis virus neutralising antibody in cats

  • D. J. Reynolds
  • D. J. Garwes
  • C. J. Gaskell
Original Papers
Received:
Accepted:

Summary

High titres of neutralising activity to transmissible gastroenteritis virus (TGEV), a porcine coronavirus, were found in sera and peritoneal fluids from cats infected with feline infectious peritonitis (FIP). A small proportion of cats, from a hospital population unaffected by FIP, also had neutralising activity. Procedures to remove non-specific viral inhibitors, including treatment by heat inactivation, trypsin, sulphydryl reagent and kaolin absorption were unsuccessful. The active component was unable to neutralise another porcine coronavirus, haemagglutinating encephalomyelitis virus or the porcine enterovirus, Talfan. Gel filtration of feline sera and peritoneal fluid demonstrated high levels of the neutralising activity in the area corresponding to 7S IgG, which could be removed by absorption with specific anti-IgG serum and these properties are suggested to be consistent with those of antibody. These findings imply that there is a coronavirus in cats which is antigenically related to TGEV and its possible nature is discussed.

Keywords

Trypsin Peritonitis Kaolin High Titre Gastroenteritis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Binn, L. N., Lazar, E. C., Keenan, K. P., Huxsoll, D. L., Marchwicki, R. H., Strano, A. J.: Recovery and characterization of a coronavirus from military dogs with diarrhoea. In: Proceedings 78th Ann. meeting, U.S. Anim. Health Assoc. Roanoke, Va., Oct. 1974. 359–366 (1975).Google Scholar
  2. 2.
    Cartwright, S., Lucas, M.: Vomiting and diarrhoea in dogs. Vet. Rec.91, 571–572 (1972).Google Scholar
  3. 3.
    Doggett, J. E., Taylor-Robinson, D., Gallop, R. G. C.: A study of an inhibitor in bovine serum active against respiratory syncytial virus. Arch. ges. Virusforsch.23, 126–137 (1968).Google Scholar
  4. 4.
    Gouffaux, M., Pastoret, P. P., Henrcteaux, M., Massip, A.: Feline infectious peritonitis. Proteins of plasma and ascitic fluids. Vet. Pathol.12, 335–348 (1975).Google Scholar
  5. 5.
    Haelterman, E. O.: Epidemiological studies of transmissible gastroenteritis of swine. Proc. U.S. Livestock Sanit. Assn.66, 305–315 (1962).Google Scholar
  6. 6.
    Horzinek, M. C., Osterhaus, A. D. M. E., Ellens, D. J.: Feline infectious peritonitis virus. I. Purification and electron microscopy. Z. Vet. Med. (In press.)Google Scholar
  7. 7.
    Joo, H. S., Donaldson-Wood, C. R., Johnson, R. H.: A microneutralization test for the assay of porcine parvovirus antibody. Arch. Virol.47, 337–341 (1975).Google Scholar
  8. 8.
    Kärber, G.: Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche. Arch. exp. path. Pharmakol.162, 480–483 (1931).Google Scholar
  9. 9.
    Lukert, P. D.: Avian infectious bronchitis virus. Characteristics of an inhibitor found in serum. Arch. ges. Virusforsch.40, 93–104 (1973).Google Scholar
  10. 10.
    McClurkin, A. W., Stark, Sharon, L., Norman, J. O.: Transmissible gastroenteritis (TGE) of swine: The possible role of dogs in the epizootiology of TGE. Canad. J. comp. Med.34, 347–349 (1970).Google Scholar
  11. 11.
    Pedersen, N. C.: Morphologic and physical characteristics of feline infectious peritonitis and its growth in autochthonous peritoneal cell cultures. Amer. J. vet. Res.37, 567–572 (1976).Google Scholar
  12. 12.
    Pedersen, N. C.: Serologic studies of naturally occurring feline infectious peritonitis. Amer. J. vet. Res.37, 1449–1453 (1976).Google Scholar
  13. 13.
    Pocock, D. H., Garwes, D. J.: The polypeptides of haemagglutinating encephalomyelitis virus and isolated subviral particles. (In preparation.)Google Scholar
  14. 14.
    Rutter, J. M., Luther, P. D.: Cytopathic factors in bacteria-free lysates ofEscherichia coli. J. med. Microbiol.6, 565–571 (1973).Google Scholar
  15. 15.
    Saif, L. J., Bohl, E. H., Gupta, R. K. P.: Isolation of porcine immunoglobulins and determination of the immunoglobulin class. Infect. Immun.6, 600–609 (1972).Google Scholar
  16. 16.
    Stone, S. S., Kemeny, L. J., Jensen, M. T.: Partial characterization of the principal soluble antigens associated with the coronavirus of transmissible gastroenteritis by complement fixation and immunodiffusion. Infect. Immun.13, 521–526 (1976).Google Scholar
  17. 17.
    Tyrrell, D. A. J., Almeida, J. D., Cunningham, C. H., Dowdle, W. R., Hofstad, M. S., McIntosh, K., Tajima, M., Zakstelskaya, L. Ya., Easterday, B. C., Kapikian, A., Bingham, R. W.: Coronaviridae. Intervirology5, 76–82 (1975).Google Scholar
  18. 18.
    Witte, K. H.: Micro-color test for assay of TGE virus-neutralizing antibodies. Arch. ges. Virusforsch.33, 171–176 (1971).Google Scholar

Copyright information

© Springer-Verlag 1977

Authors and Affiliations

  • D. J. Reynolds
    • 1
  • D. J. Garwes
    • 1
  • C. J. Gaskell
    • 2
  1. 1.Institute for Research on Animal Diseases, ComptonNewburyUnited Kingdom
  2. 2.Department of Veterinary Medicine, School of Veterinary ScienceUniversity of BristolLangford, BristolUnited Kingdom

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