Archives of Virology

, Volume 91, Issue 3–4, pp 193–205 | Cite as

Ecological and epidemiological data on Hantavirus in bank vole populations in Belgium

  • R. Verhagen
  • H. Leirs
  • E. Tkachenko
  • G. van der Groen
Original Papers


Epidemiological and ecological data of a Hantavirus infected bank vole population were collected using live-trapping methods. A close association between the distribution of HV-infected bank voles and wet habitat types was found. Several seroconversions were observed during the life of individual bank voles. All seroconversions occured within the wet habitat types and only after a certain age was reached. Survival of HV-infected bank voles did not differ from not-infected animals. In the laboratory passive antibody transfer was observed from antibody-positive females to their off-spring.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Brummer-Korvenkontio M, Vaheri A, von Bonsdorff CH, Vuorimies J, Manni T, Penttinen K, Oker-Blom N, Laehdevirta J (1980) Nephropathia epidemica: detection of antigen in bank voles and serologic diagnosis of human infection. J Infect Dis 141: 131–134Google Scholar
  2. 2.
    Brummer-Korvenkontio M, Henttonen H, Vaheri A (1982) Hemorrhagic fever with renal syndrome in Finland: Ecology and virology of Nephropathia Epidemica. Scand J Infect Dis [Suppl] 36: 88–91Google Scholar
  3. 3.
    Bujalska G (1985) Regulation of female maturation inClethrionomys species, with special reference to an island population ofC. glareolus. Ann Zool Fennici 22: 331–342Google Scholar
  4. 4.
    Gavrilovskaya I, Apekina N, Myasnikov Y, Bernshtein A, Ryltseva E, Gorbachkova E, Chumakov M (1983) Features of circulation of hemorrhagic fever with renal syndrome (HFRS) virus among small mammals in the European U.S.S.R. Arch Virol 75: 313–316Google Scholar
  5. 5.
    Gliwicz J, Rajska-Jurgiel E (1983) Social organization. Acta Theriol 28 [Suppl] 1: 134–140Google Scholar
  6. 6.
    Goldgaber D, Gibbs CJ, Gajdusek DC, Svedmyr A (1985) Definition of three serotypes of Hantavirus by a double sandwich ELISA with Biotin-Avidin amplification system. J Gen Virol 66: 1733–1740Google Scholar
  7. 7.
    Hilborn R, Redfield JA, Krebs CJ (1976) On the reliability of enumeration for mark and recapture census of voles. Can J Zool 54: 1019–1024Google Scholar
  8. 8.
    Lähdevirta J (1971) Nephropatia epidemica in Finland. A clinical, histological and epidemiological study. Ann Clin Res 3 [Suppl] 8: 1–154Google Scholar
  9. 9.
    Lähdevirta J, Savola J, Brummer-Korvenkontio M, Berndt R, Illikainen R, Vaheri A (1984) Clinical and serological diagnosis of Nephropathia epidemica, the mild type of haemorrhagic fever with renal syndrome. J Infect 9: 230–238Google Scholar
  10. 10.
    Lee HW, French GR, Lee PW, Baek LJ, Tsuchiya K, Foulke RS (1981) Observations on natural and laboratory infection of rodents with the etiologic agent of Korean hemorrhagic fever. Am J Trop Med Hyg 30 (2): 477–482Google Scholar
  11. 11.
    Lee HW (1982) Korean hemorrhagic fever. Prog Med Virol 28: 96–113Google Scholar
  12. 12.
    Nakane PK, Kawaoi A (1974) Peroxidase-labeled antibody. A new method of conjugation. J Hist Cytochem 22: 1084–1091Google Scholar
  13. 13.
    Schmaljohn CS, Hasty S, Dalrymple J, Leduc JW, Lee HW, von Bonsdorff CH, Brummer-Korvenkontio M, Vaheri A, Tsai TF, Regnery HL, Goldgaber D, Lee PW (1985) Antigenic and genetic properties of viruses linked to haemorrhagic fever with renal syndrome. Science 227: 1041–1044Google Scholar
  14. 14.
    Tkachenko EA, Ivanov A, Dzagurova TK, Donets M, Rezapkin GV, Leshchinskaya EV, Zagidulin UM, Ivanova AA, Stradukhin OV, Mustafin NM (1981) Immunosorbent assays for diagnosis of haemorrhagic fever with renal syndrome. Lancet i: 257–258Google Scholar
  15. 15.
    Tkachenko EA, Ivanov A, Donets M, Miasnikov Y, Ryltseva E, Gaponova L, Bashkirtsev V, Okulova N, Drozdov S, Slonova R (1983) Potential reservoir and vectors of haemorrhagic fever with renal syndrome (HFRS) in the U.S.S.R. Ann Soc Belge Mé Trop 63: 267–269Google Scholar
  16. 16.
    Tkachenko EA, van der Groen G, Bashkirtsev VN, Dzagurova TK, Ivanov AP (1984) Isolation of HFRS virus fromClethrionomys glareolus through the Vero-E 6 cell. Abstracts, XIth international congress for tropical medicine and malaria, Calgary, Canada, Sept. 16–22Google Scholar
  17. 17.
    van der Groen G, Piot P, Desmyter J, Colaert J, Muylle L, Tkachenko E, Ivanov A, Verhagen R, Van Ypersele de Strihou C (1983) Seroepidemiology of Hantaan related virus infections in Belgian populations. Lancet ii: 1493–1494Google Scholar
  18. 18.
    van der Groen G, Tkachenko E, Ivanov A, Verhagen R (1983) Haemorrhagic fever with renal syndrome related virus in indigenous wild rodents in Belgium. Lancet i: 110–111Google Scholar
  19. 19.
    van der Groen G, Leirs H, Verhagen R (in press) Polyhostal nature of Hantaviruses. In: Proc. sec. symp. recent advances in rodent control, Koeweit 2–6 Feb. 1985Google Scholar
  20. 20.
    van der Groen G (1985) Haemorrhagic fever with renal syndrome: recent developments. Ann Soc Belge Méd Trop 65: 121–135Google Scholar
  21. 21.
    Verhagen R (1980) A population dynamical study of the wood mouse,Apodemus sylvaticus, and the bank vole,Clethrionomys glareolus, in different habitats in Northern Antwerp. Ph. D. thesisGoogle Scholar
  22. 22.
    Verhagen R, van der Groen G, Ivanov AP, Van Rompay J, Leirs H, Verheyen W (submitted) Occurence and distribution of hantavirus in wild living mammals in Belgium. Acta Virol (Praha)Google Scholar
  23. 23.
    Xu Z, Gao C, Wu Y, Zhang X, Liu K (1985) Epidemiological studies of hemorrhagic fever with renal syndrome: Analysis of risk factors and mode of transmission. J Inf Dis 152/1: 137–144Google Scholar
  24. 24.
    Yanagihara R, Svedmyr A, Amyx H, Lee PW, Goldgaber D, Gajdusek D, Gibbs C, Nystrom K (1984) Isolation and propagation of Nephropathia epidemica virus in bank voles. Scand J Infect Dis 16: 225–228Google Scholar
  25. 25.
    Yanagihara R, Amyx H, Gajdusek D (1985) Experimental infection with Puumala virus, the etiologic agent of Nephropathia Epidemica, in Bank voles(Clethrionomys glareolus). J Virol 55: 34–38Google Scholar

Copyright information

© Springer-Verlag 1986

Authors and Affiliations

  • R. Verhagen
    • 1
  • H. Leirs
    • 1
  • E. Tkachenko
    • 3
  • G. van der Groen
    • 2
  1. 1.Laboratory of General ZoologyState University Centre AntwerpAntwerpenBelgium
  2. 2.Institute of Tropical MedicineAntwerpenBelgium
  3. 3.Institute of Poliomyelitis and Viral EncephalitidesAcademy of Medical Sciences of the U.S.S.R.MoscowU.S.S.R.

Personalised recommendations