Archives of Virology

, Volume 118, Issue 3–4, pp 225–234 | Cite as

Effect of in ovo bursectomy on the course of an infectious bronchitis virus infection in line C White Leghorn chickens

  • J. K. A. Cook
  • T. F. Davison
  • M. B. Huggins
  • P. McLaughlan
Original Papers


White Leghorn line C chicks were surgically bursectomised (Bx) in ovo to eliminate antibody production. After inoculation with infectious bronchitis virus (IBV) at 14 days after hatching, Bx chicks experienced a more severe and longer lasting infection than intact chicks. The severity and duration of clinical infection in the Bx chicks resembled that previously observed in the highly susceptible line 15 I chicks, however no increase in mortality was observed, in contrast to the high levels of mortality recorded in IBV-inoculated line 15 I chicks. After secondary challenge the degree of damage to the ciliated epithelium of the trachea was greater in the Bx chicks than in the intact chicks.

The results indicate that, although antibodies play an important role in recovery from IBV infection, other immunological factor(s) may also be involved.


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  1. 1.
    Boyle JF, Weismiller DG, Holmes KV (1987) Genetic resistance to mouse hepatitis virus correlates with absence of virus-binding activity on target tissues. J Virol 61: 185–189Google Scholar
  2. 2.
    Bumstead N, Huggins MB, Cook JKA (1989) Genetic differences in susceptibility to a mixture of avian infectious bronchitis virus andEscherichia coli. Br Poult Sci 30: 39–48Google Scholar
  3. 3.
    Cook JKA, Darbyshire JH, Peters RW (1976) The use of chicken tracheal organ cultures for the isolation and assay of avian infectious bronchitis virus. Arch Virol 50: 109–118Google Scholar
  4. 4.
    Cook JKA, Smith HW, Huggins MB (1986) Infectious bronchitis immunity: its study in chickens experimentally infected with mixtures of infectious bronchitis virus andEscherichia coli. J Gen Virol 67: 1427–1434Google Scholar
  5. 5.
    Cooper MD, Cain WA, Van Alten PJ, Good RA (1969) Development and function of the immunoglobulin producing system. Int Arch Allergy 35: 242–252Google Scholar
  6. 6.
    Davelaar FG, Kouwenhoven B (1976) Changes in the Harderian gland of the chicken following conjunctival and intranasal infection with infectious bronchitis virus in one-and 20-day-old chickens. Avian Pathol 5: 39–50Google Scholar
  7. 7.
    Davelaar FG, Kouwenhoven B (1977) Influence of maternal antibodies on vaccination of chicks of different ages against infectious bronchitis. Avian Pathol 6: 41–50Google Scholar
  8. 8.
    Gillette KG (1981) Local antibody response to avian infectious bronchitis: virus-neutralising antibody in tracheobronchial secretions. Avian Dis 25: 431–443Google Scholar
  9. 9.
    Gomez L, Raggi LG (1974) Local immunity to avian infectious bronchitis in tracheal organ culture. Avian Dis 18: 346–368Google Scholar
  10. 10.
    Gough RE, Alexander DJ (1979) Comparison of duration of immunity in chickens infected with a live infectious bronchitis vaccine by three different routes. Res Vet Sci 26: 329–332Google Scholar
  11. 11.
    Herbert WJ, Kristensen F (1986) Laboratory animal techniques for immunology. In: Weir DM (ed) Handbook of experimental immunology, vol 4, applications of immunological methods in biomedical sciences, 4th edn. Blackwell, Oxford, pp 133·1–133·36Google Scholar
  12. 12.
    Hofstad MS (1984) Avian infectious bronchitis. In: Hofstad MS, Barnes HJ, Calnek BW, Reid WH, Yoder HW Jr (eds) Diseases of poultry, 8th edn. The Iowa State University Press, Ames, Iowa, pp 429–443Google Scholar
  13. 13.
    Mockett APA, Cook JKA (1986) The detection of specific IgM to infectious bronchitis virus in chicken serum using an ELISA. Avian Pathol 15: 437–446Google Scholar
  14. 14.
    Nakamura K, Cook JKA, Otsuki K, Huggins MB, Frazier JA (1991) Comparative study of respiratory lesions in two chicken lines of different susceptibility infected with infectious bronchitis virus: histology, ultrastructure and immunohistochemistry. Avian Pathol (in press)Google Scholar
  15. 15.
    Otsuki K, Huggins MB, Cook JKA (1990) Comparison of the susceptibility to avian infectious bronchitis virus infection of two inbred lines of White Leghorn chickens. Avian Pathol 19: 467–475Google Scholar
  16. 16.
    Raggi LG, Lee GG (1965) Lack of correlation between infectivity, serologic response and challenge results in immunization with an avian infectious bronchitis vaccine. J Immunol 94: 538–543Google Scholar
  17. 17.
    Reed LJ, Muench H (1938) A simple method of estimating fifty percent end points. Am J Hyg 27: 493–497Google Scholar
  18. 18.
    Schat KA, Calnek BW, Fabricant J (1980) Influence of the bursa of Fabricius on the pathogenesis of Marek's disease. Infect Immun 31: 199–207Google Scholar
  19. 19.
    Smith HW, Cook JKA, Parsell ZE (1985) The experimental infection of chickens with mixtures of infectious bronchitis virus andEscherichia coli. J Gen Virol 66: 777–786Google Scholar
  20. 20.
    Timms LM, Bracewell CD, Alexander DJ (1980) Cell mediated and humoral immune response in chickens infected with avian infectious bronchitis. Br Vet J 136: 349–356Google Scholar
  21. 21.
    Winterfield RW, Fadly AM (1972) Some characteristics of isolates of infectious bronchitis virus from commercial vaccines. Avian Dis 16: 746–755Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • J. K. A. Cook
    • 1
  • T. F. Davison
    • 2
  • M. B. Huggins
    • 1
  • P. McLaughlan
    • 2
  1. 1.AFRC Institute for Animal HealthHoughton LaboratoryHoughton, HuntingdonUK
  2. 2.AFRC Institute for Animal HealthCompton LaboratoryCompton, NewburyUK

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