Archives of Virology

, Volume 133, Issue 3–4, pp 477–483

Enhanced replication of porcine reproductive and respiratory syndrome (PRRS) virus in a homogeneous subpopulation of MA-104 cell line

  • H. S. Kim
  • J. Kwang
  • I. J. Yoon
  • H. S. Joo
  • M. L. Frey
Brief Report

DOI: 10.1007/BF01313785

Cite this article as:
Kim, H.S., Kwang, J., Yoon, I.J. et al. Archives of Virology (1993) 133: 477. doi:10.1007/BF01313785

Summary

Two different cell populations, high- (MARC-145) and low-permissive cell clones (L-1) to porcine reproductive and respiratory syndrome (PRRS) virus, were derived from MA-104 cell line (parent cell: P) by cell cloning. Maximum virus yields in MARC-145, P, and L-1 cell clones were 108.5, 103.5, and 102.5 tissue culture infective dose 50 (TCID50)/0.1 ml, respectively. The MARC-145 cell clone supported replication of all 11 different porcine reproductive and respiratory syndrome virus isolates that were tested. These results indicated that the MARC-145 cells will be useful for PRRS virus replication.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • H. S. Kim
    • 1
  • J. Kwang
    • 1
  • I. J. Yoon
    • 2
  • H. S. Joo
    • 2
  • M. L. Frey
    • 3
  1. 1.U.S. Meat Animal Research CenterUSDA, ARS, Clay Center
  2. 2.Department of Clinical and Population Sciences, College of Veterinary MedicineUniversity of MinnesotaSt. Paul
  3. 3.Diagnostic Virology LaboratoryNational Veterinary Services Laboratories, USDA, APHISAmesUSA

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