Archives of Virology

, Volume 111, Issue 3–4, pp 281–286 | Cite as

Experimental infection of human vascular endothelial cells by pathogenic and nonpathogenic hantaviruses

  • R. Yanagihara
  • D. J. Silverman
Brief Report


To investigate whether the hantaviruses replicate in endothelial cells, we inoculated human umbilical vein endothelial cells with several pathogenic and nonpathogenic strains of hantavirus. Intracytoplasmic, virus-specific granular fluorescence was detected initially at three days postinoculation and nearly 100% of cells contained viral antigen at 10 days. Cytopathic effect or inclusion bodies were not observed. The in vitro demonstration of the susceptibility of endothelial cells to hantaviruses corroborates in vivo findings, and suggests that endothelial cells may serve as target cells in hemorrhagic fever with renal syndrome.


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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • R. Yanagihara
    • 1
    • 2
  • D. J. Silverman
    • 1
    • 2
  1. 1.Laboratory of Central Nervous System StudiesNational Institute of Neurological Disorders and Stroke, National Institutes of HealthBethesdaU.S.A.
  2. 2.the Department of MicrobiologyUniversity of Maryland, School of MedicineBaltimoreU.S.A.

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