Archives of Virology

, Volume 99, Issue 1–2, pp 45–56 | Cite as

Identification of the pseudorabies virus glycoprotein gp50 as a major target of neutralizing antibodies

  • M. Eloit
  • D. Fargeaud
  • R. L'Haridon
  • B. Toma
Original Papers


A total of 108 monoclonal antibodies specific for pseudorabies virus (PRV) were isolated in a cellular fusion, using spleen cells from mice which had been immunized with a live strain (Kojnok strain). Twelve of them neutralized the Kojnok strain in vitro in the absence of complement, as well as 28 virulent strains of various geographical origin and isolated from various animal species. All of the 12 clones were specific for glycoprotein gp50. Eighteen other clones with no neutralizing activity were studied: 11 reacted with glycoprotein GIII, 3 with glycoprotein GII, 3 with the glycoprotein gp63 and 1 with the glycoprotein GI. Transfer to mice of ascitic fluids corresponding to clones reacting with gp50 and GIII showed that some of them provided the mice with the ability of resisting to virulent challenge. Thus it appears that glycoproteins gp50 and GIII are major immunogens of the virion.


Monoclonal Antibody Infectious Disease Animal Species Virulent Strain Geographical Origin 
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  1. 1.
    Baladrachan N, Bachetti S, Rawls WE (1982) Protection against lethal challenge of Balb/c mice by passive transfer of monoclonal antibodies to five glycoproteins of herpes simplex virus type 2. Infect Immun 37: 1132–1137Google Scholar
  2. 2.
    Ben Porat T (1982) Organization and replication of Herpes virus DNA. Kaplan AS (ed) Organization and replication of viral DNA. CRC Press, Boca Raton, FL, pp 147–172Google Scholar
  3. 3.
    Ben Porat T, Demarchi J, Pendrys J, Veach RA, Kaplan AS (1986) Proteins specified by the short unique region of the genome of pseudorabies virus play a role in the release of virions from certain cells. J Virol 57: 191–196Google Scholar
  4. 4.
    Hampl H, Ben Porat T, Ehrlicher L, Habermehl KO, Kaplan S (1984) Characterization of the envelope proteins of pseudorabies virus. J Virol 52: 583–590Google Scholar
  5. 5.
    Howes EL, Clark EA, Smith E, Mitchison NA (1979) Mouse hybrid cell lines produce antibodies to herpes simplex virus type 1. J Gen Virol 44: 81–87Google Scholar
  6. 6.
    Kümmel G, Kaerner HC, Levine M, Schroeder CH, Glorioso JC (1985) Passive immune protection by herpes simplex virus specific monoclonal antibodies and monoclonal antibody-resistant mutants altered in pathogenicity. J Virol 56: 930–937Google Scholar
  7. 7.
    Laemmli UK (1970) Clivage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227: 680–685Google Scholar
  8. 8.
    Lukacs N, Thiel HS, Mettenleiter TC, Rziha HJ (1985) Demonstration of three major species of pseudorabies virus glycoproteins and identification of a disulfide-linked glycoprotein. J Virol 53: 166–173Google Scholar
  9. 9.
    Mettenleiter TC, Lukacs N, Rziha HJ (1985) Pseudorabies virus avirulent strains fail to express a major glycoprotein. J Virol 56: 307–311Google Scholar
  10. 10.
    Petrovskis EA, Timmins JG, Post LE (1986a) Use of lambda gt11 to isolate genes for two pseudorabies virus glycoproteins with homology to herpes simplex virus and varicella zoster virus glycoproteins. J Virol 60: 185–193Google Scholar
  11. 11.
    Petrovskis EA, Timmins JG, Gierman TM, Post LE (1986b) Deletions in vaccine strains of pseudorabies virus and their effect on synthesis of glycoprotein gp 63. J Virol 60: 1166–1169Google Scholar
  12. 12.
    Petrovskis EA, Timmins JG, Armentrout MA, Marchioli CC, Yancey RJ, Post LE (1986c) DNA sequence of the gene for pseudorabies virus gp 50, a glycoprotein without N-linked glycosylation. J Virol 59: 216–223Google Scholar
  13. 13.
    Rector JT, Lausch RN, Oakes JE (1982) Use of monoclonal antibodies for analysis of antibody dependant immunity of ocular herpes simplex virus type 1 infection. Infect Immun 38: 168–174Google Scholar
  14. 14.
    Robbins AK, Weis JH, Enquist LW, Watson RJ (1984) Construction of a E. Coli expression plasmid libraries: localization of a pseudorabies virus glycoprotein gene. J Mol Appl Genet 2: 485–496Google Scholar
  15. 15.
    Roizman B (1983) Herpesviruses, 2 vols. Plenum Press, New YorkGoogle Scholar
  16. 16.
    Stevely WS (1975) Virus induced proteins in pseudorabies infected cells. II. Proteins of the virion and nucleocapsid. J Virol 16: 944–950Google Scholar
  17. 17.
    Toma B (1982) Serological diagnosis of Aujeszky's disease using enzyme-linked immunosorbent assay (ELISA). In: Wittman G, Hall SA (eds) Aujeszky's disease, Brussels Luxembourg, pp 223–231Google Scholar
  18. 18.
    Volk WA, Snyder RM, Benjamin DC, Wagner RR (1982) Monoclonal antibodies to the glycoprotein of vesicular stomatitis virus: comparative neutralizing activity. J Virol 42: 200–237Google Scholar
  19. 19.
    Wathen LMK, Platt KB, Wathen MW, Van Deusen RA, Whetstone CA, Pirtle EC (1985) Production and characterization of monoclonal antibodies directed against pseudorabies virus. Virus Res 4: 19–29Google Scholar
  20. 20.
    Wathen MW, Wathen LMK (1984) Isolation, characterization and physical mapping of a pseudorabies virus mutant containing antigenically altered gp 50. J Virol 51: 57–62Google Scholar
  21. 21.
    Wathen MW, Wathen LMK (1986) Characterization and mapping of a non essential pseudorabies virus glycoprotein. J Virol 58: 173–178Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • M. Eloit
    • 1
  • D. Fargeaud
    • 2
  • R. L'Haridon
    • 3
  • B. Toma
    • 1
  1. 1.Laboratorie d'Epidémiologie et de Physiopathologie des maladies à virusMinistère de l'Agriculture, E.N.V.Maisons-AlfortFrance
  2. 2.Laboratorie IFFARhône-MérieuxLyonFrance
  3. 3.Station de VirologieMinistère de l'Agriculture, I.N.R.A.Thiverval-GrignonFrance

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