Advertisement

Archives of Virology

, Volume 101, Issue 3–4, pp 199–207 | Cite as

Hydrolysis of inositol lipids: an early signal of human cytomegalovirus infection

  • T. Valyi-Nagy
  • Z. Bandi
  • I. Boldogh
  • T. Albrecht
Original Papers

Summary

The production of inositol 1,4,5-triphosphate (IP 3) and 1,2-diacylglycerol (DG) as an early cellular response to human cytomegalovirus (HCMV) infection was investigated in human embryo lung (LU) cells. The results obtained show transient 85 and 30% increases in IP3 and DG, respectively, 20 min post-exposure to HCMV. Following heat inactivation or serum neutralization of HCMV stock or in the presence of papaverine we failed to detect increased levels of IP3 and DG relative to mock-infected LU cultures.

Keywords

Lipid Infectious Disease Inositol Cellular Response Early Signal 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Albrecht T, Weller TH (1980) Heterogeneous morphologic features of plaques induced by five strains of human cytomegalovirus. Am J Clin Pathol 73: 648–654Google Scholar
  2. 2.
    Albrecht T, Cavallo T, Cole NL, Graves K (1980) Cytomegalovirus: Development and progression of cytopathic effects in human cell culture. Lab Invest 42: 1–7Google Scholar
  3. 3.
    Albrecht T, Nachtigal M, St. Jeor SC, Rapp F (1976) Induction of cellular DNA synthesis and increased mitotic activity in Syrian hamster embryo cells abortively infected with human cytomegalovirus. J Gen Virol 30: 167–177Google Scholar
  4. 4.
    Berridge MJ (1984) Inositol triphosphate and diacylglycerol as second messenger. Biochem J 220: 345–360Google Scholar
  5. 5.
    Berridge MJ (1987) Inositol lipids and cell proliferation. Biochim Biophys Acta 907: 33–45Google Scholar
  6. 6.
    Berridge MJ, Irvine RF (1984) Inositol triphosphate, a novel second messenger in transduction. Nature 312: 315–321Google Scholar
  7. 7.
    Boldogh I, Gonczol E, Vaci L (1978) Stimulation of host DNA synthesis and induction of early antigens by ultraviolet light irradiated human cytomegalovirus. Arch Virol 58: 289–299Google Scholar
  8. 8.
    Carney DH, Scott DL, Gordon EA, LaBelle EF (1985) Phosphoinositides in mitogenesis: Neomycin inhibits thrombin stimulated phosphoinositide turnover and initiation of proliferation. Cell 42: 479–486Google Scholar
  9. 9.
    DeMarchi JM (1983) Correlation between stimulation of host cell DNA synthesis by human cytomegalovirus and lack of expression of a subset of early virus genes. Virology 129: 274–286Google Scholar
  10. 10.
    Gonczol E, Vaci L, Boldogh I (1975) The effect of supraoptimal temperature on the multiplication of different cytomegalovirus strains. Acta Microbiol Acad Sci Hung 22: 19–26Google Scholar
  11. 11.
    Lee CH, Steinsland OS, Albrecht T (1988) Cytomegalovirus: increased cyclic nucleotide levels in permissively infected fibroblasts of human origin. Submitted for publicationGoogle Scholar
  12. 12.
    Lonberg-Holm K, Philipson L (1974) Early interaction between animal viruses and cells. Monogr Virol 9: 1–149Google Scholar
  13. 13.
    Lugnier C, Stoclct JC (1974) Inhibition by papaverine of cGMP and cAMP phospho-diesterases from the rat heart. Biochem Pharmacol 23: 3071–3074Google Scholar
  14. 14.
    Michelson-Fiske S, Horodniceanu F, Guillon JC (1977) Immediate early antigens in human cytomegalovirus infected cells. Nature 270: 615–617Google Scholar
  15. 15.
    Moolenaar WH, Tsien RY, van der Saag PT, deLaat SW (1983) Na+/H+ exchange and cytoplasmic pH in the action of growth factors in human fibroblasts. Nature 304: 645–648Google Scholar
  16. 16.
    Nishizuka Y (1984) The role of protein kinase C in cell surface signal transduction and tumor promotion. Nature 308: 693–698Google Scholar
  17. 17.
    Nokta M, Eaton D, Steinsland OS, Albrecht T (1987) Ca2+ resposnes in cytomegalovirus-infected fibroblasts of human origin. Virology 157: 259–267Google Scholar
  18. 18.
    Nokta M, Fons MP, Eaton DC, Albrecht T (1988) Cytomegalovirus: sodium entry and development of cytomegaly in human fibroblasts. Virology 164: 411–419Google Scholar
  19. 19.
    Reedman BM, Klein G (1973) Cellular localization of an Epstein-Barr virus (EBV)-associated complement-fixing antigen in producer and non-producer lymphoblastoid cell lines. J Natl Canc Inst 11: 499–520Google Scholar
  20. 20.
    Rozengurt E (1986) Early signals in the mitogenic response. Science 234: 161–166Google Scholar
  21. 21.
    St. Jeor SC, Albrecht TB, Funk FD, Rapp F (1974) Stimulation of cellular DNa synthesis by human cytomegalovirus. J Virol 13: 353–362Google Scholar
  22. 22.
    Vicentini LM, Villereal ML (1984) Serum, bradykinin and vasopressin stimulate release of inositol phosphates from human fibroblasts. Biochem Biophys Res Commun 123: 663–670Google Scholar
  23. 23.
    Vonka V, Benyesh-Melnick M (1966) Interaction of human cytomegalovirus with human fibroblasts. J Bacteriol 91: 213–220Google Scholar
  24. 24.
    Wells JN, Wu YJ, Baird CE, Hardman JG (1975) Phosphodiesterases from porcine coronary arteries: Inhibition of separated forms by xanthines, papaverine and cyclic nucleotides. Mol Pharmacol 11: 775–783Google Scholar

Copyright information

© Springer-Verlag 1988

Authors and Affiliations

  • T. Valyi-Nagy
    • 1
  • Z. Bandi
    • 2
  • I. Boldogh
    • 1
  • T. Albrecht
    • 1
  1. 1.Department of MicrobiologyThe University of Texas Medical BranchGalvestonUSA
  2. 2.Department of PathologyThe University of Texas Medical BranchGalvestonUSA

Personalised recommendations