Archives of Virology

, Volume 120, Issue 1–2, pp 135–143 | Cite as

The capsid protein-encoding sequence of foot-and-mouth disease virus O2Brescia

  • O. Krebs
  • H. G. Berger
  • O. Marquardt
Brief Report

Summary

Nucleotide sequences encoding the four capsid proteins of foot-and-mouth disease virus subtype O2Brescia/1947 have been determined. These and the deduced amino acid sequences were compared with those of a subtype O1 virus strain. The nucleotide sequences differed at 259 positions, causing only 35 amino acid changes. VP 4 and VP 2 differed by 2.4 and 1.8%, whereas VP 1, known as major viral antigen, and VP 3 differed by 8% and 5.5%, respectively. The differences occur mainly in protein domains not involved in the formation of α-helices and β-sheets, suggesting that the surfaces of both viruses are more variable than their scaffolds. The O2Brescia sequence has been submitted to the GenBank data base and has the accession number M 55287.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Acharya R, Fry E, Stuart D, Fox G, Rowlands D, Brown F (1989) The three-dimensional structure of foot-and-mouth disease virus at 2.9 Å resolution. Nature 337: 709–716Google Scholar
  2. 2.
    Bachrach HL (1968) Foot-and-mouth disease. Annu Rev Microbiol 22: 201–244Google Scholar
  3. 3.
    Baxt B, Vakharia V, Moore DM, Franke AJ, Morgan DO (1989) Analysis of neutralizing antigenic sites on the surface of type A 12 foot-and-mouth disease virus. J Virol 63: 2143–2151Google Scholar
  4. 4.
    Beck E, Forss S, Strebel K, Cattaneo R, Feil G (1983) Structure of the FMDV translation initiation site and of the structural proteins. Nucleic Acids Res 11: 7873–7885Google Scholar
  5. 5.
    Beck E, Strohmaier K (1987) Subtyping of European foot-and-mouth disease virus strains by nucleotide sequence determination. J Virol 61: 1621–1629Google Scholar
  6. 6.
    Bittle JL, Houghten RA, Alexander H, Shinnick TM, Sutcliffe JG, Lerner RA (1982) Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequence. Nature 298: 30–33Google Scholar
  7. 7.
    Bolwell C, Brown AL, Barnett PV, Campbell RO, Clarke BE, Parry NR, Ouldridge EJ, Brown F, Rowlands DJ (1989) Host cell selection of antigenic variants of foot-and-mouth disease virus. J Gen Virol 70: 45–57Google Scholar
  8. 8.
    Brown F (1987) Foot-and-mouth disease virus. In: Arnon R (ed) Synthetic vaccines II. CRC Press, Boca Raton, FL, pp 65–77Google Scholar
  9. 9.
    Brown AL, Campbell RO, Clarke BE (1989) The nucleotide sequence of the structural-protein-coding region of foot-and-mouth disease virus serotype SAT 3. Gene 75: 225–234Google Scholar
  10. 10.
    Carroll AR, Rowlands DJ, Clarke BE (1984) The complete nucleotide sequence of the RNA coding for the primary translation product of foot and mouth disease virus. Nucleic Acids Res 12: 2461–2472Google Scholar
  11. 11.
    Cheung A, Delamarter J, Weiss S, Küpper H (1983) Comparison of the major antigenic determinants of different serotypes of foot-and-mouth disease virus. J Virol 48: 451–459Google Scholar
  12. 12.
    Chomczynski P, Sacci N (1987) Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal Biochem 162: 156–159Google Scholar
  13. 13.
    De Borde DC, Naeve CW, Herlocher ML, Maassab HF (1986) Resolution of a common RNA sequencing ambiguity by terminal deoxynucleotidyl transferase. Anal Biochem 157: 275–282Google Scholar
  14. 14.
    Gebauer F, De la Torre JC, Gomes I, Mateu MG, Barahona H, Tiraboschi B, Bergmann I, Auge de Mello P, Domingo E (1988) Rapid selection of genetic and antigenic variants of foot-and-mouth disease virus during persistence in cattle. J Virol 62: 2041–2049Google Scholar
  15. 15.
    Knowles NJ, Marquardt O, Samuel AR (1988) Antigenic and molecular characterization of virus isolates from recent outbreaks of foot-and-mouth disease in the Federal Republic of Germany. Report to the session of the research group of the standing technical committee of the European Commission for the Control of Foot-and-Mouth Disease. Prague, Czechoslovakia, 20–23 September, 1988Google Scholar
  16. 16.
    Krebs O, Berger H-G, Niedbalski W, Marquardt O (1991) Foot-and-mouth disease virus O1Lombardy is biochemically related to O2 isolates. Virus Gene (in press)Google Scholar
  17. 17.
    Marquardt O, Adam K-H (1988) Sequences of capsid protein VP 1 of two type A foot-and-mouth disease viruses. Virus Gene 2: 283–291Google Scholar
  18. 18.
    Marquardt O, Adam K-H (1990) Foot-and-mouth disease virus subtyping by sequencing VP 1 genes. Vet Microbiol 23: 175–183Google Scholar
  19. 19.
    Martinez MA, Carrillo C, Plana J, Mascarella R, Bergada J, Palma EL, Domingo E, Sobrino F (1988) Genetic and immunogenic variations among closely related isolates of foot-and-mouth disease virus. Gene 62: 75–84Google Scholar
  20. 20.
    Parry N, Fox G, Rowlands D, Brown F, Fry E, Acharya R, Logan D, Stuart D (1990) Structural and serological evidence for a novel mechanism of antigenic variation in foot-and-mouth disease virus. Nature 347: 569–572Google Scholar
  21. 21.
    Pfaff E, Thiel H-J, Beck E, Strohmaier K, Schaller H (1988) Analysis of neutralizing epitopes on foot-and-mouth disease virus. J Virol 62: 2033–2040Google Scholar
  22. 22.
    Robertson BH, Grubman MJ, Weddel GN, Moore DM, Welsh JD, Fischer T, Dowbenko DJ, Yamsura DG, Small B, Kleid DG (1985) Nucleotide and amino acid sequence coding for polypeptides of foot-and-mouth disease virus type A 12. J Virol 54: 651–660Google Scholar
  23. 23.
    Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-termination inhibitors. Proc Natl Acad Sci USA 74: 5463–5467Google Scholar
  24. 24.
    Smith DB, Inglis SC (1987) The mutation rate and variability of eukaryotic viruses: an analytical review. J. Gen Virol 68: 2729–2740Google Scholar
  25. 25.
    Stave JW, Card JL, Morgan DO, Vakharia VN (1988) Neutralization sites of type O1 foot-and-mouth disease virus variants. Virology 162: 21–29Google Scholar
  26. 26.
    Steinhauer DA, Holland JJ (1987) Rapid evolution of RNA viruses. Annu Rev Microbiol 41: 409–433Google Scholar
  27. 27.
    Thomas AAM, Woortmeijer RJ, Puijk W, Barteling SJ (1988) Antigenic sites on foot-and-mouth disease virus type A 10. J Virol 62: 2782–2789Google Scholar
  28. 28.
    Wittmann G (1964) Subtypen des Maul- und Klauenseuchevirus vom Typ O. Ihre antigenen und immunologischen Eigenschaften und Beziehungen zueinander. Zentralbl Bakteriol Parasitenk Infektionskrankh Hyg 194: 1–25Google Scholar
  29. 29.
    Wittmann G, Ahl R (1964) Genetische Merkmale attenuierter Maul-und Klauenseuche-Virusstämme. Bull Off Int Epizool 61: 579–589Google Scholar
  30. 30.
    Xie Q-C, McCahon D, Crowther JR, Belsham GJ, McCullough KC (1987) Neutralization of foot-and-mouth disease virus can be mediated through any of at least three separate antigenic sites. J Gen Virol 68: 1637–1647Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • O. Krebs
    • 1
  • H. G. Berger
    • 2
  • O. Marquardt
    • 1
  1. 1.Federal Research Centre for Virus Diseases of AnimalsTübingenGermany
  2. 2.Friedrich Miescher Laboratory of the Max-Planck-SocietyTübingenGermany

Personalised recommendations