Advertisement

Archives of Virology

, Volume 108, Issue 3–4, pp 259–270 | Cite as

Pattern of anti-cytomegalovirus IgM antibodies determined by immunoblotting

A study of kidney graft recipients developing a primary or recurrent CMV infection
  • J. Basson
  • J. C. Tardy
  • M. Aymard
Original Papers

Summary

In order to improve the knowledge of the humoral immune response to CMV infection, we developed an immunoblotting technique which allowed a better analysis of the changes in the pattern of anti CMV-polypeptides IgM. We examined 234 sera belonging to 27 renal allograft recipients developing a primary or recurrent CMV infection and 12 non infected recipients. Thus we found that 11 main anti CMV-polypeptides IgM antibodies were present in over 25% of the infected patients. They reacted with proteins whose molecular weights ranged from 32K to 205 K.

We showed that anti-p 45–47 IgM antibodies were present in 100% of CMV infected recipients and never in the non-infected population. They appeared very early in the course of the infection (5.43 weeks post-graft for primary infection and 5.00 weeks for recurrent ones) and, therefore, constitute a good marker of active infection. Two other CMV-specific IgM antibodies (anti-p 60–64 and anti-p 100) were found exclusively in the course of primary infections. Anti-p 60–64 IgM was observed at a high frequency (57.1%) and with a mean delay of 6.57 weeks post-graft. Therefore, the anti-p 60–64 IgM detection could be helpful for the diagnosis of primary infection. In almost 100% of both primary and recurrent infections, we observed anti-p 140 and anti-p 38 IgM antibodies. Only about 50% of non-infected patients had low levels of anti-p 140 and anti-p 38 IgM.

The follow-up of recurrent infections showed that the anti CMV-polypeptides IgM antibodies appeared earlier than in primary infection. When we compared anti-p 45–47 IgM detection by immunoblotting and anti-CMV IgM detected by ELISA we observed that immunoblotting permitted the diagnosis 2.5 weeks earlier for primary infection, and 1 week earlier for recurrent infection, than ELISA. In addition, the detection of anti-p 45–47 IgM antibodies also occurred earlier than virus excretion.

Keywords

Humoral Immune Response Primary Infection Recurrent Infection Renal Allograft Good Marker 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Aymard M, Bosshard S, Chomel JJ, Gibert R, El Yasi M, Langlois M, Malik MC, Revillard JP, Tardy JC, Touraine JL, Traeger J (1982) Herpesvirus infections in patients with renal allografts. In: Touraine JL et al (eds) Transplantation and clinical immunology, vol 14. Excerpta Medica, Amsterdam, p 113–120Google Scholar
  2. 2.
    Fiala M, Honess RW, Heiner DC, Heine JW, Murnane J, Wallace R, Guze LB (1976) Cytomegalovirus proteins: I. polypeptides of virions and dense bodies. J Virol 19: 243–254Google Scholar
  3. 3.
    Forman SJ, Zaia JA, Clark BR, Wright CL, Mills BJ, Pottathil R, Racklin BC, Gallagher MT, Welte K, Blume KG (1985), A 64,000 dalton matrix protein of human cytomegalovirus induces in vitro immune response similar to those of whole viral antigen. J Immunol 134: 3391–3395Google Scholar
  4. 4.
    Gibson W (1981) Structural and non structural proteins of strain Colburn cytomegalovirus. Virology 111: 516–537Google Scholar
  5. 5.
    Gibson W (1983) Protein counterparts of human and simian cytomegaloviruses. Virology 128: 391–406Google Scholar
  6. 6.
    Glenn J (1981) Cytomegalovirus infections following renal transplantation. Rev Infect Dis 3: 1151–1173Google Scholar
  7. 7.
    Gupta P, St Jeor S, Rapp F (1977) Comparison of the polypeptides of several strains of human cytomegalovirus. J Gen Virol 34: 447–454Google Scholar
  8. 8.
    Horodniceanu F, Michelson S (1980) Assessment of human cytomegalovirus antibody detection technique. Brief Review. Arch Virol 64: 287–301Google Scholar
  9. 9.
    Kim KS, Sapienza VJ, Carp RJ, Moon HM (1976) Analysis of structural polypeptides of purified human cytomegalovirus. J Virol 20: 604–611Google Scholar
  10. 10.
    Laemmli UK (1970) Cleavage of structural proteins during the asssembly of the head of bacteriophage T 4. Nature 227: 680–685Google Scholar
  11. 11.
    Landini MP, Re MC, Mirolo G, Baldassarri B, La Placa M (1985) Human immune response to cytomegalovirus structural polypeptides studied by immunoblotting. J Med Virol 17: 303–311Google Scholar
  12. 12.
    Pereira L, Hoffman M, Cremer N (1982) Electrophoretic analysis of polypeptides immune precipitated from cytomegalovirus-infected cell extracts by human sera. Infect Immun 36: 933–942Google Scholar
  13. 13.
    Porath A, Hanuka N, Keynan A, Sarov I (1987) Virus-specific serum IgG, IgM and IgA antibodies in cytomegalovirus mononucleosis patients as determined by immunoblotting technique. J Med Virol 22: 223–230Google Scholar
  14. 14.
    Revello MG, Percivalle E, Gerna G (1986) Immunoglobulin M to the membrane of uninfected fibroblasts in primary human cytomegalovirus infections. Microbiologica 9: 127–138Google Scholar
  15. 15.
    Sarov I, Abady I (1975) The morphogenesis of human cytomegalovirus isolation and polypeptide characterization of cytomegalovirions and dense bodies. Virology 66: 464–473Google Scholar
  16. 16.
    Schmitz H, Muller-Lantzsch N, Peteler G (1980) Human response to proteins of cytomegalovirus. Intervirology 13: 154–161Google Scholar
  17. 17.
    Stinski MF (1978) Sequence of protein synthesis in cells infected by human cytomegalovirus: early and late virus induced polypeptides. J Virol 26: 686–701Google Scholar
  18. 18.
    Tardy JC, Pouteil-Noble C. Touraine JL, Aymard M (1987) Prognostic value of anti cytomegalovirus IgM in kidney graft recipients. In: Brynger H (ed) Transplantation proceedings, vol 19. Grune and Stratton, Philadelphia, p 4066–4067Google Scholar
  19. 19.
    Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76: 4350–4354Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • J. Basson
    • 1
  • J. C. Tardy
    • 2
  • M. Aymard
    • 1
  1. 1.Laboratoire de Virologie du Centre Hospitalier UniversitaireLyonFrance
  2. 2.Département d'Étude des Maladies ViralesLaboratoire National de la SantéLyonFrance

Personalised recommendations