Archives of Virology

, Volume 79, Issue 3–4, pp 173–187 | Cite as

Replication of HSV-1 in murine peritoneal macrophages: Comparison of various virus strains with different properties

  • J. Görtz
  • B. Brake
  • V. Härle-Grupp
  • D. Falke
Original Papers
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Accepted:

Summary

Thein vitro replication of eleven different strains of herpes simplex virus type 1 was studied in resident or thioglycollate-stimulated mouse macrophages. The strains of herpes simplex virus differed in the type of cytopathic effect, induction capacity for herpes simplex virus coded thymidine kinase and pathogenicity in the mouse. Herpes simplex virus replicated better in thioglycollate-stimulated macrophages than in resident macrophages.In vitro ageing of macrophages increased their replicative potency. Herpes simplex virus replicated better in macrophages from homozygous bg/bg C57/BL6J mice than in macrophages from their heterozygous littermates. Separation of macrophages on discontinous Percoll-gradients revealed 4 fractions with identical potency for replication. The ability of herpesvirus to replicate in macrophages varied from strain to strain of virus i.e. Wal > Len, clone 4 of Len, >L3-2s, JES, Ang−, Ang+path, clone 2 of Len and >MDK clones. The ability to cause cytopathology also varied. Only strains Ang− and Ang+path showed limited or late cytopathology in macrophages. The cell-fusing property of herpes simplex virus appeared to be more closely correlated with lower replication rates than production cell rounding. Thymidine kinase− viruses replicated less well than thymidine kinase+ or thymidine kinase(+) strains. Strains of herpes simplex virus with high or low pathogenicity for mice replicated in macrophages to the same degree. The phagocytic activity of macrophages for IgM-coated sheep red blood cells was inhibited earlier by strains of herpes simplex virus of type 2 than by strains of herpes simplex virus of type 1.

Keywords

Herpes Simplex Virus Strain Herpes Simplex Virus Type Peritoneal Macrophage Thymidine Kinase 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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References

  1. 1.
    Armerding, D., Mayer, P., Scriba, M., Hren, A., Rossiter, H.:In vivo modulation of macrophage functions by herpes simplex virus type 2 in resistant and sensitive inbred mouse strains. Immunobiol.160, 217–227 (1981).Google Scholar
  2. 2.
    Beller, D. I., Jiely, J. M., Unanue, E. R.: Regulation of macrophage population: I. Preferential induction of Ia-rich peritoneal exudates by immunologic stimuli. J. Immunol.124, 1426–1432 (1980).Google Scholar
  3. 3.
    Beller, D. I., Unanue, E. R.: Regulation of macrophage populations: II. Synthesis and expression of Ia-antigens by peritoneal exudate macrophages is a transient event. J. Immunol.126, 263–269 (1981).Google Scholar
  4. 4.
    Boot, I. R., Sweatman, W. I. F., Cox, B. A., Stone, K., Dawson, W.: The anti-allergic activity of benoxaprofen (2-(4-chlorophenyl)-α-methyl-5 benzoxozole acetic acid) — a lipooxygenase inhibitor. Int. Archs. Allergy appl. Immun.67, 340–343 (1982).Google Scholar
  5. 5.
    Collier, L. H., Scott, Q. J., Pani, A.: Variation in resistance of cells from inbred strains of mice to herpes simplex virus type 1. J. gen. Virol.64, 1483–1490 (1983).Google Scholar
  6. 6.
    Domke, I., Brücher, J., Schröder, C. H., Kirchner, H.: The role of interferon in thein vitro replication of herpes simplex virus (HSV) type 1 in mouse macrophages. 8th International Herpesvirus Workshop, 31.7.—5. 8. 1983, Oxford (1983).Google Scholar
  7. 7.
    DuBuy, H.: Effect of silica on virus infections in mice and mouse tissue culture. Infect. Immun.11, 996–1002 (1975).Google Scholar
  8. 8.
    Eustatia, J. M., Maase, E., van Helden, P., van der Veen, J.: Viral replication in mouse macrophages. Arch. ges. Virusforsch.39, 376–380 (1972).Google Scholar
  9. 9.
    Falke, D.: unpublished.Google Scholar
  10. 10.
    Field, H. J., Wildy, P.: The pathogenicity of thymidine kinase-deficient mutants of herpes simplex virus in mice. J. Hyg. Camb.81, 267–277 (1978).Google Scholar
  11. 11.
    Frank, U., Schindling, B., Lindermann, J., Falke, D.: Multiplication of HSV types 1 and 2 in macrophages of NMRI and C57/BL mice. Acta Virol.22, 193–202 (1978).Google Scholar
  12. 12.
    Hirsch, M. S., Zisman, B., Allison, A. C.: Macrophages and age-dependent resistance to herpes simplex virus in mice. J. Immunol.104, 1155–1172 (1970).Google Scholar
  13. 13.
    Jamieson, A. T., Gentry, G. A., Subak-Sharpe, J. H.: Induction of both thymidine and deoxycytidine kinase activity by Herpesviruses. J. gen. Virol.24, 465–480 (1974).Google Scholar
  14. 14.
    Kaerner, H. C., Schröder, Ch., Ott-Hartmann, A., Kümel, G., Kirchner, H.: Genetic variability of HSV: Development of a pathogenic variant during passaging of a non-pathogenic HSV-1 virus strain in mouse brain. J. Virol.46, 83–93 (1983).Google Scholar
  15. 15.
    Kirchner, H.: Suppressor cells of immune reactivity in malignancy. Europ. J. Cancer14, 453–459 (1978).Google Scholar
  16. 16.
    Kirchner, H.: Immunobiology of infection with Herpes simplex virus. In:Melnick, J. L. (ed.), Monographs in Virology, Vol. 13. Basel: S. Karger 1982.Google Scholar
  17. 17.
    Knoblich, A., Görtz, J., Härle-Grupp, V., Falke, D.: Kinetics and Genetics of HSV-induced antibody formation in mice. Infect. Immun.39, 15–23 (1983).Google Scholar
  18. 18.
    Knoblich, A., Kampe, P., Härle-Grupp, V., Falke, D.: Aspects of regulation of antibody formation against HSV-1 and 2 in the mouse. 8th International Herpesvirus Workshop, 31. 7.—5. 8. 1983, Oxford (1983).Google Scholar
  19. 19.
    Labenz, J., Friedrich, D., Falke, D.: Analysis of the TK enzyme complex induced by HSV types 1 and 2 by means of isoelectric focusing and polyacrylamide gel electrophoresis. Arch. Virol.71, 235–249 (1982).Google Scholar
  20. 20.
    Lopez, C., Dudas, G.: Replication of herpes simplex virus type 1 in macrophages from resistant and susceptible mice. Infect. Immun.23, 432–437 (1979).Google Scholar
  21. 21.
    Lopez, C.: Resistance to herpes simplex virus-type 1 (HSV-1). Curr. Topics in Microbiol. and Immunol.92, 15–24 (1981).Google Scholar
  22. 22.
    Maecock, S. C. R., Kitchen, E. A.: Effects of the non-steroidal anti-inflammatory drug benoxaprofen on leucocyte migration. J. Pharm. Pharmacol.31, 366–370 (1979).Google Scholar
  23. 23.
    Morahan, P. S., Morse, S. S., McGeorge, M.: Macrophage extrinsic antiviral activity during herpes simplex virus infection. J. gen. Virol.46, 291–300 (1980).Google Scholar
  24. 24.
    Müller, W. E. G., Schuster, D. K., Zahn, R. K., Maidhof, A., Leyhausen, G., Falke, D., Koren, R., Umezawa, H.: Properties and specificity of binding sites for the immunomodulator bestatin on the surface of mammalian cells. Intern. J. Immunopharmac.4, 393–400 (1982).Google Scholar
  25. 25.
    Sethi, K. K., Brandis, H.:In vitro acquisition of resistance against herpes simplex virus by permissive murine macrophages. Arch. Virol.59, 157–172 (1979).Google Scholar
  26. 26.
    Schröder, C. H., Engler, H., Kirchner, H.: Protection of mice by an apathogenic strain of HSV-1 against lethal infection by a pathogenic strain of HSV-1. J. gen. Virol.52, 159–161 (1981).Google Scholar
  27. 27.
    Walker, W. S.: Separation of mouse resident peritoneal cells into subpopulations on discontinous density gradients of percoll. In:Herscowitz, H. B., Holden, H. T., Bellanti, J. A., Ghaffar, A. (eds.), Manual of macrophage methodology; collection, characterization and function, 100–102. New York: Marcel Dekker 1981.Google Scholar
  28. 28.
    Wellek, B., Hahn, H., Opferkuch, W.: Quantitative contributions of IgG, IgM and C3 to erythrophagocytosis and rosette formation by peritoneal macrophages, and opsonin activity of dextransulfate 500. Eur. J. Immunol.5, 378–381 (1975).Google Scholar
  29. 29.
    Wildy, P., Gell, P. G. H., Rhodes, J., Newton, A.: Inhibition of herpes simplex virus multiplication by activated macrophages: a role for arginase? Infect. Immun.37, 40–50 (1982).Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • J. Görtz
    • 1
  • B. Brake
    • 1
  • V. Härle-Grupp
    • 1
  • D. Falke
    • 1
  1. 1.Institut für Medizinische MikrobiologieAbteilung für Experimentelle Virologie der UniversitätMainzGermany

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