Archives of Virology

, Volume 139, Issue 3–4, pp 431–438

Mycoplasma stimulates HIV-1 expression from acutely- and dormantly-infected promonocyte/monoblastoid cell lines

  • M. I. H. Chowdhury
  • T. Munakata
  • Y. Koyanagi
  • S. Arai
  • N. Yamamoto
Brief Report

DOI: 10.1007/BF01310804

Cite this article as:
Chowdhury, M.I.H., Munakata, T., Koyanagi, Y. et al. Archives of Virology (1994) 139: 431. doi:10.1007/BF01310804

Summary

Treatment of a myelo-monocyte cell line, J22HL-60, dormantly infected with human immunodeficiency virus type 1 (HIV-1) with heat-inactivated extracts ofAcholeplasma (A) laidlawii (250 µg/ml) enhanced virus production more than 45-fold as assessed by p24 viral core antigen assay. When treated with a suboptimal dose of TPA or TNF-α, Acholeplasma extracts further augmented virus production in J22HL-60 cells. H7, an inhibitor of protein kinase C(PKC), almost completely abrogated HIV-1-inducing ability of Acholeplasma extracts in the cells.A. laidlawii and several other mycoplasmas also enhanced acute infection of U937 cells as shown by increased virus-positive cells and augmentation of HIV-1 production in the culture supernatant independent of their pathogenicity to humans.

Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • M. I. H. Chowdhury
    • 1
  • T. Munakata
    • 2
  • Y. Koyanagi
    • 1
  • S. Arai
    • 2
  • N. Yamamoto
    • 1
  1. 1.Department of MicrobiologyTokyo Medical and Dental University School of MedicineTokyo
  2. 2.Department of BacteriologyKurume University School of MedicineFukuokaJapan

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