Archives of Virology

, Volume 97, Issue 1–2, pp 123–130 | Cite as

Protection from mouse hepatitis virus type 3-induced acute disease by an anti-nucleoprotein monoclonal antibody

  • J. Lecomte
  • V. Cainelli-Gebara
  • G. Mercier
  • S. Mansour
  • P. J. Talbot
  • G. Lussier
  • D. Oth
Brief Report


Fusion of MHV-3-immune splenocytes from MHV-3-resistant A/J murine strain, with NS myeloma cells produced several hybridomas. Among eight hybridoma clones, the 1E7A4H1 clone secreted kappa IgG2a apparently directed against the nucleoprotein of the MHV-3 virion. The monoclonal antibody was able to neutralize the in vitro cytopathic effect of MHV-3 on cultured L2 cells, and was detected by indirect immuno-fluorescence on MHV-3-infected cultured YAC cells. In addition, it conferred a significant protection against MHV-3-induced acute disease, if injected intraperitoneally to C57BL/6 mice before inoculation with MHV-3.


Hepatitis Myeloma Hepatitis Virus Myeloma Cell Cytopathic Effect 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© by Springer-Verlag 1987

Authors and Affiliations

  • J. Lecomte
    • 1
  • V. Cainelli-Gebara
    • 2
  • G. Mercier
    • 2
  • S. Mansour
    • 2
  • P. J. Talbot
    • 1
  • G. Lussier
    • 1
  • D. Oth
    • 2
  1. 1.Centre de recherche en virologieInstitut Armand-FrappierVille de LavalCanada
  2. 2.Centre de recherche en immunologieInstitut Armand-FrappierVille de LavalCanada

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