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Archives of Virology

, Volume 110, Issue 1–2, pp 91–101 | Cite as

Dengue-2 virus infection of human mononuclear cell lines and establishment of persistent infections

  • I. Kurane
  • U. Kontny
  • J. Janus
  • F. A. Ennis
Original Papers

Summary

Twenty three human mononuclear cell lines including ten myelomonocytic cell lines, eight B cell lines and five T cell lines, were examined to determine whether they could be infected with dengue-2 virus. All the cell lines were infected with dengue-2 virus as determined by immunofluorescent staining and by virus titration of culture supernatant fluids. K 562, Jiyoye and Jurkat, respectively, showed the highest percentage of infected cells of these myelomonocytic, B and T cell lines. Antibody to dengue-2 virus at subneutralizing concentrations augmented dengue-2 virus infection of myelomonocytic cell lines, but not of B cell lines or of T cell lines.

Persistent dengue-2 virus infection was established using a myelomonocytic cell line (K562), a B cell line (Raji), and a T cell line (HSB-2). These cell lines maintained a high percentage (more than 70%) of dengue-2 virus antigen-positive cells for at least 25 weeks. Very low titers of infectious dengue-2 virus were detected in the culture supernatant fluids of the persistently infected cells. Dengue-2 virus antigen-positive Raji cell clones were established from persistently-infected Raji cells using limiting dilutions and all of the cells in these clones were dengue-2 virus antigen-positive. These findings demonstrate that a variety of human mononuclear cell lines can be infected with dengue-2 virus and may be useful as models for the analysis of dengue virus-human cell interactions in dengue virus infections.

Keywords

Infectious Disease Virus Infection Infected Cell Immunofluorescent Staining Cell Clone 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    Adams RA, Flowers A, Davis BJ (1968) Direct implantation serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res 28: 1121–1125Google Scholar
  2. 2.
    Anderson CL, Looney RJ (1986) Human leukocyte IgG Fc receptors. Immunol Today 7: 164–266Google Scholar
  3. 3.
    Boonpucknavig S, Boonpucknavig V., Bhamarapravati N., Nimmannitya S (1979) Immunofluorescence study of skin rash in patients with dengue hemorrhagic fever. Arch Pathol Lab Med 103: 463–466Google Scholar
  4. 4.
    Boonpucknavig V, Bhamarapravati N, Boonpucknavig S, Futrakul P, Tanpaichitr P (1976) Glomerular changes in dengue hemorrhagic fever. Arch Pathol Lab Med 100: 206–212Google Scholar
  5. 5.
    Brandt WE, McCown JM, Gentry MK, Russell PK (1982) Infection enhancement of dengue type 2 virus in the U-937 human monocyte cell line by antibodies to flavivirus cross-reactive determinants. Infect Immun 36: 1036–1041Google Scholar
  6. 6.
    Burk KH, Drewinko B, Trujillo JM, Ahearn MJ (1978) Establishment of human plasma cell line in vitro. Cancer Res 38: 2508–2513Google Scholar
  7. 7.
    Burke DS, Nisalak A, Johnson DE, Scott RM (1988) A prospective study of dengue infections in Bangkok. Am J Trop Med Hyg 38: 172–180Google Scholar
  8. 8.
    Collins SJ, Gallo RC, Gallagher RE (1977) Continuous growth and differentiation of human myeloid leukemic cells in suspension culture. Nature 270: 347–349Google Scholar
  9. 9.
    Epstein MA, Barr YM (1965) Characteristics and mode of growth of a tissue culture strain (EB1) of human lymphoblasts from Burkitt's lymphoma. J Nat Cancer Inst 34: 231–240Google Scholar
  10. 10.
    Fahey JL, Buell DN, Sox HC (1971) Proliferation and differentiation of lymphoid cells: studies with human lymphoid cells and immunoglobulin synthesis. Ann NY Acad Sci 190: 221–234Google Scholar
  11. 11.
    Foley GE, Lazarus H, Farber S, Uzman BG, Boone BA, McCarthy RE (1965) Continuous culture of human lymphoblasts from peripheral blood of a child with acute leukemia. Cancer 18: 522–529Google Scholar
  12. 12.
    Halstead SB (1980) Immunological parameters of togavirus disease syndrome. In: Schlesinger RW (ed) The Togaviruses: biology, structure, replication. Academic Press, New York, p 107Google Scholar
  13. 13.
    Halstead SB (1981) The pathogenesis of dengue: molecular epidemiology in infectious disease. Am J Epidemiol 114: 632–648Google Scholar
  14. 14.
    Halstead SB (1981) Dengue hemorrhagic fever—a public health problem and a field for research. Bull WHO 58: 1–21Google Scholar
  15. 15.
    Halstead SB, O'Rourke EJ (1977) Dengue virus and mononuclear phagocytes. I. Infection enhancement by non-neutralizing antibody. J Exp Med 146: 201–217Google Scholar
  16. 16.
    Halstead SB, O'Rourke EJ, Allison AC (1977) Dengue virus and mononuclear phagocytes. II. Identity of blood and tissue leukocytes supporting in vitro infection. J Exp Med 146: 218–229Google Scholar
  17. 17.
    Harris NS (1974) Plasma cell surface antigen on human plasma lymphocytes. Nature 250: 507–509Google Scholar
  18. 18.
    Klein E, Klein G, Nadkarni S, Nadkarni JJ, Wigzell H, Clifford P (1968) Surface IgM-kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines. Cancer Res 28: 1300–1310Google Scholar
  19. 19.
    Klein G, Giovanella B, Westman A, Stehlin JS, Mumford D (1975) An EBV-genome-negative cell line established from an American Burkitt lymphoma; receptor characteristics, EBV infectivity and permanent conversion into EBV-positive sublines by in vitro infection. Intervirology 5: 319–334Google Scholar
  20. 20.
    Koeffler HP, Golde DW (1978) Acute myelogenous leukemia: a human cell line responsive to colony-stimulating activity. Science 200: 1153–1154Google Scholar
  21. 21.
    Kurane I, Ennis FA (1987) Induction of interferon from human lymphocytes by autologous, dengue virus-infected monocytes. J Exp Med 166: 999–1010Google Scholar
  22. 22.
    Kurane I, Hebblewaite D, Brandt W, Ennis FA (1984) Lysis of dengue-infected cells by natural cell-mediated cytotoxicity and antibody-dependent cell-mediated cytotoxicity. J Virol 52: 223–230Google Scholar
  23. 23.
    Kurane I, Meager A, Ennis FA (1986) Induction of interferon alpha and gamma from human lymphocytes by dengue virus-infected cells. J Gen Virol 67: 1653–1661Google Scholar
  24. 24.
    Kurane I, Meager A, Ennis FA (1989) Dengue virus-specific human T cell clones: serotype crossreactive proliferation, interferon gamma production, and cytotoxic activity. J Exp Med 170: 763–775Google Scholar
  25. 25.
    Looney RJ, Abraham GN, Anderson CL (1986) Human monocytes and U937 cells bear two distinct Fc receptors for IgG. J Immunol 136: 1641–1647Google Scholar
  26. 26.
    Lozzio CB, Lozzio BB (1975) Human chronic myelogenous leukemia cell-line with positive Philadelphia chromosome. Blood 45: 321–334Google Scholar
  27. 27.
    Magrath IT, Pizzo PA, Whang-Peng J, Douglass EC, Alabaster O, Gerber P, Freeman CB, Novikovs L (1980) Characterization of lymphoma-derived cell lines: comparison of cell lines positive and negative for Epstein-Barr Virus nuclear antigen. I. Physical, cytogenetic, and growth characteristics. J Nat Cancer Inst 64: 465–473Google Scholar
  28. 28.
    Martin P, Papayannopoulou T (1982) HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science 216: 1233–1235Google Scholar
  29. 29.
    Minowada J, Ohnuma T, Moore GE (1972) Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes. J Nat Cancer Inst 49: 891–895Google Scholar
  30. 30.
    Monath TP (1985) Flaviviruses. In: Fields BN, Knipe DM, Chanock RM, Roizman B, Shope R (eds) Virology. Raven Press, New York, p 955Google Scholar
  31. 31.
    Ohta M, Furukawa Y, Ide C, Akiyama N, Utakoji T, Miura Y, Saito M (1986) Establishment and characterization of four human monocytoid leukemia cell lines (JOSK-I, -S, -M, and -K) with capabilities of monocyte-macrophage lineage differentiation and constitutive production of interleukin 1. Blood 46: 3067–3074Google Scholar
  32. 32.
    Pulvertaft RJV (1965) A study of malignant tumours in Nigeria by short-term tissue culture. J Clin Pathol 18: 261–273Google Scholar
  33. 33.
    Scott RM, Nisalak A, Cheamudon U, Seridhoranakul S, Nimmannitya S (1980) Isolation of dengue viruses from peripheral blood leukocytes of patients with hemorrhagic fever. J Infect Dis 141: 1–6Google Scholar
  34. 34.
    Sundstrom C, Nilsson K (1976) Establishment and characterization of a human histiocytic lymphoma cell line (U-937). Int J Cancer 17: 565–577Google Scholar
  35. 35.
    Sung JS, Diwan AR, Falkler WA Jr, Yang H-Y, Halstead SB (1975) Dengue carrier culture and antigen production in human lymphoblastoid lines. Intervirology 5: 137–149Google Scholar
  36. 36.
    Theofilopoulos AN, Brandt WE, Russell PK, Dixon FT (1976) Replication of dengue-2 virus in cultured human lymphoblastoid cells and subpopulations of human peripheral leukocytes. J Immunol 117: 953–961Google Scholar
  37. 37.
    Tsuchiya S, Yamabe M, Yamaguchi Y, Kobayashi Y, Konno T, Tada K (1980) Establishment and characterization of a human acute monocytic leukemia cell line (THP-1). Int J Cancer 26: 171–176Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • I. Kurane
    • 1
  • U. Kontny
    • 1
  • J. Janus
    • 1
  • F. A. Ennis
    • 1
  1. 1.Division of Infectious Diseases, Department of MedicineUniversity of Massachusetts Medical CenterWorcesterU.S.A.

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