Archives of Virology

, Volume 110, Issue 1–2, pp 91–101 | Cite as

Dengue-2 virus infection of human mononuclear cell lines and establishment of persistent infections

  • I. Kurane
  • U. Kontny
  • J. Janus
  • F. A. Ennis
Original Papers


Twenty three human mononuclear cell lines including ten myelomonocytic cell lines, eight B cell lines and five T cell lines, were examined to determine whether they could be infected with dengue-2 virus. All the cell lines were infected with dengue-2 virus as determined by immunofluorescent staining and by virus titration of culture supernatant fluids. K 562, Jiyoye and Jurkat, respectively, showed the highest percentage of infected cells of these myelomonocytic, B and T cell lines. Antibody to dengue-2 virus at subneutralizing concentrations augmented dengue-2 virus infection of myelomonocytic cell lines, but not of B cell lines or of T cell lines.

Persistent dengue-2 virus infection was established using a myelomonocytic cell line (K562), a B cell line (Raji), and a T cell line (HSB-2). These cell lines maintained a high percentage (more than 70%) of dengue-2 virus antigen-positive cells for at least 25 weeks. Very low titers of infectious dengue-2 virus were detected in the culture supernatant fluids of the persistently infected cells. Dengue-2 virus antigen-positive Raji cell clones were established from persistently-infected Raji cells using limiting dilutions and all of the cells in these clones were dengue-2 virus antigen-positive. These findings demonstrate that a variety of human mononuclear cell lines can be infected with dengue-2 virus and may be useful as models for the analysis of dengue virus-human cell interactions in dengue virus infections.


Infectious Disease Virus Infection Infected Cell Immunofluorescent Staining Cell Clone 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • I. Kurane
    • 1
  • U. Kontny
    • 1
  • J. Janus
    • 1
  • F. A. Ennis
    • 1
  1. 1.Division of Infectious Diseases, Department of MedicineUniversity of Massachusetts Medical CenterWorcesterU.S.A.

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