Archives of Virology

, Volume 80, Issue 2–3, pp 119–130 | Cite as

Differing cardiotropic and myocarditic properties of group B type 4 coxsackievirus strains

  • Yiyun Cao
  • D. P. Schnurr
  • Nathalie J. Schmidt


A murine model system for evaluation of myocarditic and cardiotropic properties of strains of group B, type 4 coxsackievirus (CBV-4) was developed in male BALB/c mice 4 weeks of age. Differing cardiotropic and myocarditic properties could be identified among field strains within the CBV-4 serotype. These properties were consistent for the virus strains, and were independent of the infecting virus dose. Virus replication in the heart appeared to be essential for development of myocarditis, but some infected hearts with high levels of infectious virus did not show myocarditis. Two of the myocarditic strains showed different histopathology in infected hearts; with one strain (Mil) the myocarditis was characterized by a marked inflammatory reaction with occasional accompanying myofiber necrosis. With the other strain (Bol), necrosis was the predominant finding, with a much lesser degree of inflammation. These findings suggest that there may be various pathogenic or immunopathogenic mechanisms by which CBV-4 can produce myocarditis.


Infectious Disease Virus Replication Inflammatory Reaction Murine Model Myocarditis 


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  1. 1.
    Burch, G. E., DePasquale, N. P., Sun, S. C., Hale, A. R., Mogabgab, W. J.: Experimental coxsackievirus endocarditis. J. Amer. Med. Assn.196, 349–352 (1966).Google Scholar
  2. 2.
    Choppin, P. W., Eggers, H. J.: Heterogeneity of Coxsackie B4 virus: Two kinds of particles which differ in antibody sensitivity, growth rate, and plaque size. Virology18, 470–476 (1962).Google Scholar
  3. 3.
    El-Khatib, M. R., Chason, J. L., Ho, K.-L., Silberberg, B., Lerner, A. M.: Coxsackievirus B4 myocarditis in mice: valvular changes in virus-infected and control animals. J. Infect. Dis.137, 410–420 (1978).Google Scholar
  4. 4.
    Gray, F. G.: Spontaneous cardiac lesions in mice. Their bearing on attempts to produce experimental carditis. Am. J. Pathol.25, 1215–1225 (1949).Google Scholar
  5. 5.
    Grist, N. R.: Coxsackie virus infections of the heart. In:Waterson, A. P. (ed.), Recent Advances in Clinical Virology, 141–150. London: Churchill Livingstone 1977.Google Scholar
  6. 6.
    Grodums, E. I., Dempster, G.: The pathogenesis of Coxsackie group B viruses in experimental infection. Canad. J. Microbiol.8, 105–113 (1962).Google Scholar
  7. 7.
    Hartig, P. C., Madge, G. E., Webb, S. R.: Diversity within a human isolate of Coxsackie B4: Relationship to viral-induced diabetes. J. Med. Virol.11, 23–30 (1983).Google Scholar
  8. 8.
    Khatib, R., Chason, J. L., Silberberg, B. K., Lerner, A. M.: Age-dependent pathogenicity of group B coxsackieviruses in Swiss-Webster mice: Infectivity for myocardium and pancreas. J. Infect. Dis.141, 394–403 (1980).Google Scholar
  9. 9.
    Minnich, L. L., Ray, C. G.: Variable susceptibility of mice to group B coxsackievirus infections. J. Clin. Microbiol.11, 73–75 (1980).Google Scholar
  10. 10.
    Rytel, M. W., Kilbourne, E. D.: Differing susceptibility of adolescent and adult mice to non-lethal infection with coxsackievirus B3. Proc. Soc. Exp. Biol. & Med.137, 443–448 (1971).Google Scholar
  11. 11.
    Wigand, R., Sabin, A. B.: Intratypic antigenic heterogeneity of Coxsackie B viruses. Arch. Virusforsch.12, 29–41 (1962).Google Scholar
  12. 12.
    Wilson, F. M., Lerner, A. M.: A cardiomyopathy of BALB/c mice (superimposed infection by Coxsackievirus B-3). Proc. Soc. Exp. Biol. Med.157, 442–448 (1978).Google Scholar
  13. 13.
    Woodruff, J. F.: Viral myocarditis. Amer. J. Pathol.101, 425–483 (1980).Google Scholar

Copyright information

© Springer-Verlag 1984

Authors and Affiliations

  • Yiyun Cao
    • 1
  • D. P. Schnurr
    • 2
  • Nathalie J. Schmidt
    • 3
  1. 1.Institute of Medical BiologyChinese Academy of Medical SciencesKunmingPeople's Republic of China
  2. 2.Center for Advanced Medical TechnologySan Francisco State UniversitySan Francisco
  3. 3.Viral and Rickettsial Disease LaboratoryCalifornia Department of Health ServicesBerkeleyU.S.A.

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