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Archives of Virology

, Volume 111, Issue 1–2, pp 87–101 | Cite as

The budding of defective human immunodeficiency virus type 1 (HIV-1) particles from cell clones persistently infected with HIV-1

  • T. Goto
  • K. Ikuta
  • J. J. Zhang
  • C. Morita
  • K. Sano
  • M. Komatsu
  • H. Fujita
  • S. Kato
  • M. Nakai
Original Papers

Summary

Three cell clones producing large numbers of infectious or noninfectious particles of human immunodeficiency virus type 1 (HIV-1), designated M 10/LAV-2, M 16/LAV-3, and MT/LAV-17, were isolated from persistently HIV-1-infected MT-4 cells. In M 10/LAV-2, the HIV-1 proteins were defective in the cleavage ofgag precursor protein, and the particles were doughnutshaped with a double-ring structure. These particles were produced by budding at the cell surface from crescentic structures followed by the formation of double-ring structures. The viral proteins in M 16/LAV-3 were defective in the cleavage ofenv precursor protein. The morphology of the virus particles was intact, and an electron dense bar-shaped core was seen inside a single-ring enveloped structure. The intact particles were released from the cell surface by a budding process in which crescent shape structures first appeared at the cell membrane, then subsequently just before release matured to a complete structure with an electron dense core. In MT/LAV-17, the synthesis of HIV-1 proteins was normal, and the particles were teardrop-shaped with an intact core structure. These particles were produced by budding with an electron dense core at the cell surface.

Thus, it was suggested that the morphological maturation of HIV-1 particles was completed just before release from the cell surface in several cell clones producing HIV-1 particles of different morphology.

Keywords

Precursor Protein Cell Clone Shape Structure Intact Core Crescent Shape 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • T. Goto
    • 1
  • K. Ikuta
    • 2
  • J. J. Zhang
    • 1
  • C. Morita
    • 1
  • K. Sano
    • 1
  • M. Komatsu
    • 4
  • H. Fujita
    • 4
  • S. Kato
    • 3
  • M. Nakai
    • 1
  1. 1.Department of MicrobiologyOsaka Medical CollegeTakatsuki, Osaka
  2. 2.Division of Serology, Institute of Immunological ScienceHokkaido UniversityKita-ku, Sapporo
  3. 3.Department of Pathology, Research Institute for Microbial DiseasesOsaka UniversitySuita, OsakaJapan
  4. 4.Research Center of UHVEMOsaka UniversitySuita, OsakaJapan

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