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Archives of Virology

, Volume 117, Issue 1–2, pp 97–107 | Cite as

Identification in porcine faeces of a novel virus with a bisegmented double stranded RNA genome

  • J. E. Ludert
  • Mayra Hidalgo
  • F. Gil
  • F. Liprandi
Original Papers

Summary

Polyacrylamide gel electrophoresis of nucleic acids extracted from porcine faecal samples revealed in several samples the presence of two discrete bands. The bands were resistant to digestion with DNase I and RNase T1, but not with RNase A in low salt conditions, indicating that they consisted of double stranded (ds) RNA. The two bands from different samples varied in sizes, in a range between 2.4–2.6 kbp and 1.7–1.9 kbp for the slow and fast moving band respectively. The bands cosedimented in CsCl gradients at an average density of 1.415 g/ml with icosahedral virus particles of a diameter of 34nm and a triangulation number equal to 3. Aggregates of virus, which appeared to be immunocomplexes, were seen in one sample. From 244 faecal samples collected in one farm, 27 (11.1%) were found to contain the characteristic dsRNA pattern, with a higher prevalence in samples from animals 15 to 35 days old. The agent was equally distributed among samples from diarrhoeic or non-diarrhoeic animals. These results confirm the circulation among pigs of a novel virus, possibly of vertebrates, with a bisegmented double stranded RNA genome, similar to viruses previously described in humans, wild rats, guinea pigs, pigs, and chickens, for which the name “picobirnavirus” has been proposed.

Keywords

Nucleic Acid Polyacrylamide Faecal Sample Virus Particle Average Density 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • J. E. Ludert
    • 1
  • Mayra Hidalgo
    • 1
  • F. Gil
    • 1
  • F. Liprandi
    • 1
  1. 1.Laboratorio Biologia de VirusCentro de Microbiologia, Instituto Venezolano de Investigaciones Cientificas (IVIC)CaracasVenezuela

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