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Archives of Virology

, Volume 120, Issue 3–4, pp 289–296 | Cite as

Intraepidemic variants of influenza virus H3 hemagglutinin differing in the number of carbohydrate side chains

  • W. Seidel
  • F. Künkel
  • B. Geisler
  • W. Garten
  • B. Herrmann
  • L. Döhner
  • H. -D. Klenk
Brief Report

Summary

During the epidemic outbreak in the region of Greifswald in the winter 1974/75, we found influenza virus variants which showed differences in the electrophoretic mobility of HA. Among the 25 isolates 13 were of slower and 12 of higher mobility. HA1 of 6 isolates was studied by determining the number of the carbohydrate side chains and by direct sequencing of vRNA. Evidence is presented that variants showing a slower electrophoretic mobility of HA1 had consistently acquired a seventh carbohydrate side chain at Asn 126 in epitope A. All the isolates differed from the reference strain A/Port Chalmers/1/73 by the loss of the oligosaccharide at Asn 81. The field strain A/Dresden/3/71 possessed only 5 oligosaccharides in HA1. These results suggest that changes in glycosylation are an important mechanism in the structural variation underlying antigenic drift of HA.

Keywords

Carbohydrate Infectious Disease Influenza Oligosaccharide Influenza Virus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • W. Seidel
    • 1
  • F. Künkel
    • 2
  • B. Geisler
    • 1
  • W. Garten
    • 2
  • B. Herrmann
    • 1
  • L. Döhner
    • 1
  • H. -D. Klenk
    • 2
  1. 1.Institut für medizinische MikrobiologieErnst-Moritz-Arndt-UniversitätGreifswaldFederal Republic of Germany
  2. 2.Institut für VirologiePhilipps-UniversitätMarburgFederal Republic of Germany

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