Archives of Virology

, Volume 92, Issue 1–2, pp 55–62 | Cite as

Evaluation of the use of monoclonal antibodies to hemagglutinin and fusion glycoproteins of Newcastle disease virus for virus identification and strain differentiation purposes

  • G. Meulemans
  • M. Gonze
  • M. C. Carlier
  • P. Petit
  • A. Burny
  • Le Long
Original Papers

Summary

Monoclonal antibodies detect evident antigenic variations in NDV HN and F protein. However, the A/PMV-1 viruses can be identified by HI test using a preparation made of the combination of two different monoclonals.

A primary evaluation of the pathogenicity of the isolated viruses can be made by HI test using monoclonal antibodies but needs always confirmation using conventional pathogenicity tests.

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References

  1. 1.
    Alexander DJ (1982) Avian paramyxoviruses-other than Newcastle disease virus. World's Poult Sci J 38: 97–105Google Scholar
  2. 2.
    Alexander DJ, Russell PH, Collins MS (1984) Paramyxovirus type 1 infections of racing pigeons: 1 characterization of isolated viruses. Vet Rec 114: 444–446Google Scholar
  3. 3.
    Allan WH, Gough RE (1974) A standard hemagglutination inhibition test for Newcastle diseases. 1. A comparison of macro and micro methods. Vet Rec 95: 120–123Google Scholar
  4. 4.
    Allan WH, Lancaster JE, Toth B (1978) Newcastle disease vaccines. Food and Agriculture Organization of the United Nations, RomeGoogle Scholar
  5. 5.
    Arias-Ibarrondo J, Mikami T, Yamamoto H, Furuta Y, Ishioka S (1978) Studies on a paramyxovirus isolated from Japanes sparrow-hawks(Accipter virgatus gularis). Jpn J Vet Sci 40: 315–323Google Scholar
  6. 6.
    Bankowski RA, Kinjo J (1965) Tissue culture systems with Newcastle disease virus and relationship of antigenicity to immunogenicity among strains. Avian Dis 9: 157–170Google Scholar
  7. 7.
    Gomez-Lillo M, Bankowski RA, Wiggins AD (1974) Antigenic relationships among viscerotropic-velogenic and domestic strains of Newcastle disease virus. Am J Vet Res 35: 471–475Google Scholar
  8. 8.
    Hannoun C (1977) Isolation from birds of influenza viruses with human neuraminidase. Biol Standard 39: 461–468Google Scholar
  9. 9.
    Ishida M, Nerome K, Matsumoto M, Mikami T, Oya A (1985) Characterization of Reference strains of Newcastle Disease virus (NDV) and NDV-like isolates by monoclonal antibodies to HN subunits. Arch Virol 85: 109–121Google Scholar
  10. 10.
    Le Long, Portetelle D, Ghysdael J, Gonze M, Burny A, Meulemans G (1986) Monoclonal antibodies to hemagglutinin and fusion glycoproteins of Newcastle disease virus: relationship between glycosylation and reactivity. J Virol 57: 1198–1202Google Scholar
  11. 11.
    Meulemans G, Gonze M, Carlier MC, Petit P, Burny A, Le Long (1986) Anti-genic and biological characterization of avian paramyxovirus type 1 isolates from pigeons. Arch Virol 87: 151–161Google Scholar
  12. 12.
    Nishikawa K, Isomura S, Suzuki S, Watanabe E, Hamaguchi M, Yoshida T, Nagai Y (1983) Monoclonal antibodies to the HN glycoprotein of Newcastle disease virus. Biological characterization and use for strain comparisons. Virology 130: 318–330Google Scholar
  13. 13.
    Pennington TH (1978) Antigenic differences between strains of Newcastle disease virus. Arch Virol 56: 345–351Google Scholar
  14. 14.
    Reed LJ, Muench H (1938) A simple method of estimating fifty percent end points. Am J Hyg 27: 493–497Google Scholar
  15. 15.
    Russell PH, Alexander DJ (1983) Antigenic variation of Newcastle disease virus strains detected by monoclonal antibodies. Arch Virol 75: 243–253Google Scholar
  16. 16.
    Schloer G (1974) Antigenic relationships among Newcastle disease virus mutants obtained from laboratory strains and from recent California isolates. Infect Immun 10: 724–732Google Scholar
  17. 17.
    Schloer G, Spalatin J, Hanson RP (1975) Newcastle disease virus antigens and strain variations. Am J Vet Res 36: 505–508Google Scholar

Copyright information

© Springer-Verlag 1987

Authors and Affiliations

  • G. Meulemans
    • 1
  • M. Gonze
    • 1
  • M. C. Carlier
    • 1
  • P. Petit
    • 1
  • A. Burny
    • 2
  • Le Long
    • 2
  1. 1.National Institute for Veterinary ResearchBrussels
  2. 2.Department of Molecular BiologyUniversity of BrusselsBrusselsBelgium

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